Drought-resistance-related ssr sequences from abnormal cotton and their applications

An abnormal and drought-resistant technology, applied in the field of SSR sequences related to drought resistance, can solve the problems of limited utilization of cotton diploid wild species, achieve rapid identification accuracy and stability, speed up the breeding process, improve selection efficiency and breeding speed Effect

Active Publication Date: 2022-07-12
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports at home and abroad on the genetic improvement of upland cotton using diploid wild cotton. However, due to the differences in chromosome ploidy level and genetic structure, the use of diploid wild cotton in upland cotton genetic improvement is still very limited. of

Method used

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  • Drought-resistance-related ssr sequences from abnormal cotton and their applications
  • Drought-resistance-related ssr sequences from abnormal cotton and their applications
  • Drought-resistance-related ssr sequences from abnormal cotton and their applications

Examples

Experimental program
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Embodiment 1

[0059] Example 1. Determination of chromosome fragments related to cotton drought resistance traits and development of related molecular markers

[0060] The drought-resistant cotton in the present invention is cultivated by the following steps:

[0061] Using upland cotton 86-1 as the donor, crossed with the male abnormal cotton to obtain triploid F 1 (A 1 D 1 B 1 ), fertile hexaploid F was obtained by treating the young axillary buds of triploid seedlings with 0.15% colchicine 1 (A 1 A 1 D 1 D 1 B 1 B 1 );

[0062] Taking the hexaploid as the female parent, it was crossed with the parent upland cotton su 8289 to obtain the pentaploid A 1 A 1 D 1 D 1 B 1 , planting all the seeds received, resulting in 97 individual plants;

[0063] The obtained pentaploid was backcrossed with Su8289 to obtain BC 2 F 1 384 individual plants were obtained, with 50 recombination types, of which 36 recombination types were fertile;

[0064] will BC 2 F 1 All individuals of Su...

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Abstract

The invention discloses a drought resistance-related SSR sequence derived from abnormal cotton and its application. The present invention provides the application of a specific DNA fragment or its related biological material in cultivating drought-resistant cotton varieties or lines or improving the drought resistance of cotton; the specific DNA fragment is located on chromosome 5 of abnormal cotton and contains at least the self-SSR molecular marker JAAS6365 (SEQ Fragments from ID No. 1) to SSR molecular marker JAAS5604 (SEQ ID No. 2); the relevant biological materials are vectors, expression cassettes, recombinant bacteria or transgenic cell lines containing the specific DNA fragments. The chromosome fragment provided by the invention has important application value in drought-resistant cotton breeding, and the SSR molecular marker developed by the invention provides a molecular basis for breeding drought-resistant cotton varieties that can be applied to production.

Description

technical field [0001] The invention relates to the field of cotton breeding, in particular to a drought resistance-related SSR sequence derived from abnormal cotton and its application. Background technique [0002] Cotton is an important economic crop and raw material for the textile industry in the world, and its production status has an important impact on the development of the world economy. In recent years, with the continuous deterioration of the global climate and the destruction of the ecological environment, the area of ​​saline-alkali arid areas has become larger and larger. There are four cultivars in the genus Gossypium spp., of which upland cotton (Gossypium hirsutum L.) dominates world cotton production due to its high yield. However, due to long-term artificial selection, upland cotton has a narrow genetic base, and its drought resistance is stronger than other crops, but drought and desertification still cause persistent damage to cotton yield and fiber qu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 徐珍珍徐鹏沈新莲郭琪孟珊
Owner JIANGSU ACAD OF AGRI SCI
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