Piao chicken rumpless gene, and method, primer and kit for detecting rumpless trait of Piao Chicken

A gene detection and trait technology, applied in the field of mutation or genetic engineering, can solve the problems of indistinguishability of tailless chickens, difficulties in conservation and breeding of chickens, and unclear genetic rules and genetic basis of tailless chickens. High accuracy, time saving, low cost effect

Inactive Publication Date: 2019-02-01
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the genetic law and genetic basis of the tailless trait in the goose chicken are still unclear
In particular, the tailless trait of the pheasant is a dominant trait, and it is difficult to distinguish the homozygous genotype and the heterozygous genotype of the pheasant from phenotypic identification, which brings great difficulties to the conservation and breeding of the pheasant

Method used

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  • Piao chicken rumpless gene, and method, primer and kit for detecting rumpless trait of Piao Chicken
  • Piao chicken rumpless gene, and method, primer and kit for detecting rumpless trait of Piao Chicken
  • Piao chicken rumpless gene, and method, primer and kit for detecting rumpless trait of Piao Chicken

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Chicken hybridization test and its genetic law

[0037] Hybridization experiment 1: The hybridization experiment of Piaoji roosters and White Rock hens was carried out in Xiaosiao Progenitor Broiler Breeder Farm in Yunnan Province. The male parent of the cross-breeding combination comes from the Tailless Scooter Hen of Yunling Guangda Piao Chicken Original Breeding Conservation Farm in Zhenyuan, and the female parent is the White Rock hen of the Tego matching line.

[0038] Hybridization experiment 2: the hybridization experiment of Bailuo rooster and Piaoji hen, which was carried out in Yunling, Zhenyuan Guangda Piaoji Original Species Preservation Farm. The male parent of the hybrid combination is the Di Gao mating line White Rock rooster from the Xiaosiao Progenitor Broiler Breeder Farm in Yunnan Province, and the female parent is the koala hen.

[0039] Hybridization experiment 3: The hybridization experiment of Piaoji roosters and Hailan layer hens was c...

Embodiment 2

[0048] The acquisition of embodiment 2 chicken IRX1 gene polymorphic sites

[0049] 1 Chicken blood sample collection

[0050] In the sample collection, the sex, age, feather color, tail traits and pedigree information of each chicken were recorded in detail, and 2-3ml of whole blood was collected from the wing vein using a heparin sodium anticoagulated vacuum blood collection tube, transported to the laboratory at 4°C, and then Store at -20°C.

[0051] 2 Genomic DNA Extraction

[0052] Chicken wing vein blood collection, all blood samples were 1mL / bird, anticoagulated with EDTA, and the blood samples were frozen at -20°C. Adopt conventional phenol-chloroform method to extract genomic DNA from above-mentioned chicken blood, detect with 1.0% agarose electrophoresis, can see that DNA band is clear, bright, without miscellaneous band, fully meets the requirement of follow-up PCR test ( figure 2 ).

[0053] 3 Primer design and synthesis

[0054] According to the IRX1 gene co...

Embodiment 3I

[0070] Example 3 Association analysis of single SNP of IRX1 gene and tailless trait

[0071] The association analysis between a single SNP and chicken tail development was analyzed by one-way analysis of variance in SPSS. If P<0.05, it was significant.

[0072] Using the logistic regression method to analyze the correlation between single SNPs of the IRX1 gene and the tailless trait of the chicken under the codominant mode of inheritance, it was found that the SNP2 and SNP5 loci were significantly correlated with the tailless trait of the chicken (P0.05). IRX1 gene SNP3 site genotype G / G tailed group frequency (95.5%) is extremely significantly higher than the no-tailed group 0 (0%) frequency, suggesting that G / G type is a tailed genotype; IRX1 gene SNP8 site The frequency (95.5%) of the tailed group of genotype A / A is extremely significantly higher than the 0 (0%) frequency of the tailless group, suggesting that type A / A is a tailed genotype. The results are shown in Table 6....

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Abstract

The invention belongs to the technical field of mutation or genetic engineering, DNA or RNA involved in genetic engineering, and vectors, and discloses a Piao chicken rumpless gene, and a method, a primer and a kit for detecting the rumpless trait of Piao chicken, and provides two detection markers Mbo II and Ava II for Piao chicken IRX1 gene. According to the present invention, on the basis of the genome-wide association analysis, all the genes in the relevant interval are subjected to sequence determination and genetic variation analysis by using the position cloning technology, such that the results show that the IRX1 gene is the functional gene of the rumpless trait of Piao chicken, the simple, rapid, effective, time-saving and low-cost PCR-RFLP detection markers are established, and the positive guiding significance is provided for the purification of the Piao chicken rumpless gene and the establishment of the breeding system; and the two markers can be used for the gene detectionof the rumpless trait of Piao chicken and the molecular marker-assisted breeding, and have characteristics of early screening, rapid identification, time saving, low cost, high accuracy and the like.

Description

technical field [0001] The invention belongs to the field of mutation or genetic engineering; DNA or RNA involved in genetic engineering, and the technical field of vectors, and in particular relates to a tailless gene of a pheasant chicken and a method for detecting the trait of a pheasant pheasant, a primer and a kit. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Piao chicken is a unique and rare chicken species found in China during the investigation of livestock and poultry species resources during the "Eleventh Five-Year Plan" period. Its production area is Zhenyuan County, Pu'er City, Yunnan Province, and adjacent counties such as Ning'er County, Jingdong County, and Jinggu Small distribution. Because of the lack of tail feathers, including tail feathers and large and small sickle feathers, the scoop-shaped chicken is called "scoop chicken", and the locals also call it "group chicken". It was found b...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 史宪伟王欣陈多珍李杨杨向东罗袁山
Owner YUNNAN AGRICULTURAL UNIVERSITY
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