The invention provides a
multiplex amplification
system based on rapid
mutation Y-STR
gene loci, a method and application. The
multiplex amplification
system comprises 13
gene loci, namely, DYF387S1, DYF399S1, DYF403S1a / b, DYF404S1, DYS449, DYS518, DYS526b, DYS547, DYS570, DYS576, DYS612, DYS626 and DYS627, wherein sequences of amplification primer pairs are shown as SEQ ID NO.1-26, and the
gene loci are marked through four
fluorescein of 6-FAM, VIC, NED and PET respectively. According to the
multiplex amplification
system based on the rapid
mutation Y-STR gene loci, the method and the application, for the 13 rapid
mutation Y-STR gene loci, the primers are designed on the basis of
group frequency survey, and the gene loci are integrated into the multiplex
reaction system according to specific combination and primer concentration, so that amplification and detection of the gene loci are achieved simultaneously, and the amplification effect is better; when the multiplex amplification system is applied to the detection process, convenience and rapidness are better achieved, the
typing result is clear, the
correctness, sensitivity and stability of gene locus detection can be guaranteed, and higher individual recognition rate is achieved.