SSR molecular marker associated with cotton short stalk and high lint percentage

A molecular marker and lint technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of difficulty in cotton yield, environmental pollution, time-consuming and labor-intensive, and achieve rapid and accurate identification. stability, speed up the breeding process, increase the selection efficiency and the effect of breeding speed

Active Publication Date: 2019-08-06
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of a large amount of chemical reagents such as benzedramine is not only time-consuming and labor-intensive, but also causes a certain degree of pollution to the environment.
[0003] The number of bolls per plant, boll weight and cotton yield are the three major factors tha

Method used

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  • SSR molecular marker associated with cotton short stalk and high lint percentage
  • SSR molecular marker associated with cotton short stalk and high lint percentage
  • SSR molecular marker associated with cotton short stalk and high lint percentage

Examples

Experimental program
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Example Embodiment

[0051] Example 1. Determination of chromosome segments related to cotton short stem and high lint traits and development of related molecular markers

[0052] The low-stalk high-grain cotton in the present invention is cultivated through the following steps:

[0053] Using upland cotton 86-1 as the donor, crossed with abnormal male parent cotton to obtain triploid F 1 (A 1 D 1 B 1 ), hexaploid F is obtained by treating the tender axillary buds of triploid seedlings with 0.15% colchicine 1 (A 1 A 1 D 1 D 1 B 1 B 1 );

[0054] Using the hexaploid as the female parent, crossed with the parent Su 8289 to obtain Pentaloid A 1 A 1 D 1 D 1 B 1 , Planted all the received seeds and got 97 individual plants;

[0055] The obtained pentaploid is backcrossed with Su 8289, and BC is obtained 2 F 1 All the seeds were planted, and 384 individual plants were obtained, with 50 recombination types, 36 of which were fertile;

[0056] Will BC 2 F 1 All individuals of and backcrossed with Su 8289 and obtaine...

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Abstract

The invention discloses a SSR molecular marker associated with cotton short stalk and high lint percentage. The present invention provides a chromosome fragment derived from abnormal cotton, which isthe fragment located on the chromosome 11 of the abnormal cotton from the SSR molecular marker NAU2877 to the SSR molecular marker JAAS4311; a nucleotide sequence of the SSR molecular marker NAU2877 is shown as SEQ ID No. 1; and a nucleotide sequence of the SSR molecular marker JAAS4311 is shown as SEQ ID No. 2. The chromosome fragment provided by the invention has important application value in cotton breeding of short stalk and high lint percentage, and the developed SSR molecular marker provides a molecular basis for cultivating the cotton variety of short stalk and high lint percentage which can be applied for production.

Description

Technical field [0001] The present invention relates to the field of cotton breeding, in particular to SSR molecular markers related to cotton short stem and high lint content. Background technique [0002] Cotton is an important economic crop and raw material for the textile industry in my country, and it has an irreplaceable position in the national economy. In recent years, with changes in the domestic and international economic situation and the adjustment of national industrial policies, the area of ​​cotton planting has fallen sharply in the Yangtze and Yellow River basins. The main reasons are as follows: planting cotton is more time-consuming and labor-intensive than other field crops, and production costs increase; the process of urbanization and industrialization is accelerated, a large amount of labor is transferred to cities, and there is a shortage of rural labor. Therefore, improving the level of mechanization and large-scale production is the only way for the deve...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐珍珍孟珊沈新莲徐鹏郭琪张香桂
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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