Method for increasing disease-resistant breeding efficiency of hybrid rice
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A hybrid rice, efficient technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, etc. The effect of improving efficiency
Inactive Publication Date: 2017-05-10
FUJIAN AGRI & FORESTRY UNIV
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[0002] Rice molecular marker-assisted breeding is currently a commonly used method for rice disease resistance breeding, but at present, among the rice varieties that have passed the provincial-level approval in China, there are few varieties bred by molecular marker-assisted breeding.
The main reasons for the above situation are as follows: (1) At present, many domestic breeding units, especially grass-roots breeding units, do not have the conditions or technology to use molecular marker-assisted breeding technology for disease-resistant breeding; There are not many sources, and the efficiency of disease resistance breeding is not high
In addition, the identification of rice blast resistance is greatly affected by the environment, and there are problems such as unstable identification results and low efficiency.
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Embodiment 1
[0027] 1) Put Modan (with resistance to rice blast Pb-1 ) And CBB23 (carrying bacterial blight resistance gene Xa23 ) Hybridization, the offspring use with Pb-1 Gene and Xa23 Screening of gene-linked molecular markers to obtain Xa23 + Pb-1 ”Germplasm of chromosome fragments. The salt-tolerant mutant (carrying salt-tolerant gene sst , Obtained from R401 radiation) and Teqing (carrying resistance to rice blast Pi-tq1 ) Cross, the offspring use the same method to obtain the sst + Pi-tq1 ”Germplasm B of chromosome segment;
[0028] 2) Combine Yixiang 1B and 1) germplasm A ( Xa23 + Pb-1 ) To cross, get F 1 seed;
[0029] 3) Planting 2) F obtained 1 . When heading, take Yixiang 1B as the female parent, F 1 For the male parent, crossbreed, get BC 1 F 1 seed;
[0030] 4) Planting 3) BC obtained 1 F 1 seed. At the seedling stage, 120 individual plants resistant to bacterial blight were screened by Bacterial blight P6. After heading, 10 individual plants with other traits more consist...
Embodiment 2
[0047] 1) Longke 638S and Yixiangkang 2B obtained in Example 1 ( Xa23 + Pb-1 ) Hybrid, get F 1 seed;
[0048] 2) Plant F 1 , Late harvest, get F 2 seed;
[0049] 3) Planting 2) Income F 2 seed. In the seedling stage, the bacterial leaf blight fungus P6 was used to screen 1,000 individual plants resistant to bacterial blight. In the later stage, the fertility and agronomic traits were selected according to conventional breeding methods, and 100 sterile individual plants were obtained and self-bred to F 3 seed;
[0050] 4) Plant according to the plot 3) Income F 3 seed. In the seedling stage, the bacterial blight fungus P6 was used to screen 35 regions of bacterial blight resistant and non-segregating strains. In the later period, 30 strains were selected for agronomy, yield traits, and fertility by pedigree method, and self-bred F 4 seed;
[0051] 5) Planting 4) Income F 4 seed. In the seedling stage, the bacterial blight-resistant strain P6 was used to screen 30 areas of bacterial...
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Abstract
The invention discloses a method for increasing the disease-resistant breeding efficiency of hybrid rice, and belongs to the field of disease-resistant breeding of the hybrid rice. The method comprises the following steps: creating germplasm containing a bacterial blight resistance gene and rice blast resistance gene chromosome segment or a salt resistance gene and rice blast resistance gene chromosome segment by utilizing a molecular marker-assisted selection technology, and respectively hybridizing with a three-line maintainer line, a two-line sterile line or a restoring line; screening resistant individual plants for each segregative generation in a seedling stage by utilizing bacterial blight germs until screening out a new maintainer line B-resistant plant, a two-line sterile line S-resistant plant or a new restoring line R-resistant plant which obtains genetic stability and accords with breeding purposes; matching by using a sterile line containing the bacterial blight resistance gene and rice blast resistance gene chromosome segment and a restoring line containing the salt resistance gene and rice blast resistance gene chromosome segment, thus obtaining a hybrid variety containing the bacterial blight resistance gene, the rice blast resistance gene and the salt resistance gene. The method provided by the invention is simple and quick, and the disease-resistant breeding efficiency of the hybrid rice is increased.
Description
Technical field [0001] The invention specifically relates to a method for improving the disease-resistant fertility efficiency of hybrid rice, and belongs to the field of disease-resistant breeding. Background technique [0002] Rice molecular marker-assisted breeding is a commonly used method for rice disease resistance breeding. However, among the rice varieties that have been certified at or above the provincial level, few varieties have been bred by molecular marker-assisted breeding. The main reasons for the above situation are: (1) At present, many domestic breeding units, especially grassroots breeding units, do not have the conditions or technology to use molecular marker assisted breeding technology for disease resistance breeding; (2) At present, they carry multiple disease resistance genes. There are not many sources, and the efficiency of disease-resistant breeding is not high. In addition, the identification of rice blast resistance is greatly affected by the enviro...
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