A Molecular Marker Closely Linked to the Common Bean Anthracnose Resistance Gene Locus and Its Detection Method

An anthracnose and kidney bean technology, which is applied in the field of molecular markers and their detection, can solve the problems of affecting the quality and yield of common kidney beans, lack of molecular markers for disease-resistant breeding, and harming kidney beans, thereby saving planting costs, improving efficiency, and significantly economical effect of benefit

Inactive Publication Date: 2016-04-20
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

As the main miscellaneous grain crop in my country, common bean has a wide range of planting areas, but it is often harmed by bean anthracnose in production. It is especially serious in a cool and humid environment, and the yield is reduced by 30% to 90%. In the popular year, the plants wilt and die, which seriously affects the quality and yield of common beans
So far, 20 anthracnose genes or QTLs have been mapped, but there is a lack of molecular markers that can be effectively used in disease resistance breeding

Method used

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  • A Molecular Marker Closely Linked to the Common Bean Anthracnose Resistance Gene Locus and Its Detection Method
  • A Molecular Marker Closely Linked to the Common Bean Anthracnose Resistance Gene Locus and Its Detection Method
  • A Molecular Marker Closely Linked to the Common Bean Anthracnose Resistance Gene Locus and Its Detection Method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the acquisition of molecular marker SNP+In / Del / Pro7

[0025] 1. Test materials

[0026] Common kidney bean high anthracnose-resistant germplasm red kidney bean, uniform number: F00002322; anthracnose-susceptible germplasm Jingdou, uniform number: F00000777; and its hybrid F00000777 2:3 group. The above materials are all from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, and published in the document "Mingli Chen, Jing Wu, Lanfeng Wang, Xiaoyan Zhang, Matthew W.Blair, JizengJia, Shumin Wang.

[0027] 2. Identification of common bean resistance to anthracnose

[0028] For parents F00002322, F00000777 and 1,092 F 2:3 Family (each F 2:3 After the plant is sown with 30 seeds from the family, when the three-outer compound leaves of the plant are fully unfolded, the high-pressure spray inoculation method is used to inoculate the whole plant for identification at the seedling stage. Cultured in artificial climate room temperature 20...

Embodiment 2

[0040] Embodiment 2, the verification of molecular marker SNP+In / Del / Pro7

[0041] The results of resistance identification showed that: common bean Michelite, uniform number: F00005600, from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, showed high sensitivity to anthracnose race 81. In this study, the high anthracnose resistant variety F00002322 and the high susceptible variety F00005600 were used to construct a hybrid combination. By identifying the hybrid combination F00005600 2 The consistency of the resistance phenotype and genotype of the isolated population was further verified by the marker SNP+In / Del / Pro7. Specific steps are as follows:

[0042] 1. Hybrid the F00002322×F00005600 of common kidney bean 2 A single seed of the substitute seed was sown in a nutrient pot. When the three-leaf compound leaf was fully expanded, a piece of the triple-leaf compound leaf was collected to extract genomic DNA. After the next day, the plant was inoculat...

Embodiment 3

[0047] Example 3. Marker genotype analysis of molecular marker SNP+In / Del / Pro7 on common bean resistant materials

[0048] The 10 disease-resistant resources and 8 disease-susceptible resources used for the detection of markers in this experiment were provided by the Institute of Crop Science, Chinese Academy of Agricultural Sciences. Academy of Sciences, 2008.” published in.

[0049] The calculation method of the disease index and the method of classifying the resistance of common beans to anthracnose according to the disease index refer to the literature "Wang Kun. Molecular markers and localization research on the anthracnose resistance gene of common common bean. Chinese Academy of Agricultural Sciences, 2008." method.

[0050] According to the survey results of a single plant, the disease index is calculated using the formula DI=∑(Ci×Ni) / 9N, where DI is the disease index; Ci is the disease level of a single plant, and the disease classification of a single plant is the s...

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Abstract

The invention discloses a molecular marker closely interlocked with an anti-anthracnose gene locus of common beans and a detection method of the molecular marker. The molecular marker consists of DNA molecules shown by SEQ ID No.1 and SEQ ID No.2. Results show that by adopting the molecular marker to perform PCR amplification on DNA of a genome of a common bean to be detected, whether the common bean to be detected can resist bean anthracnose or not can be effectively detected according to the size of an amplification product. By adopting the method, the process of seed selection of new anti-anthracnose species can be accelerated, the efficiency of breeding for disease resistance can be improved, environmental pollution caused by pesticide control of anthracnose can be reduced, and meanwhile, the planting cost is lowered, so that the method has obvious economic and ecological benefits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker closely linked to an anthracnose resistance gene locus of common bean and a detection method thereof. Background technique [0002] Common bean (Phaseolus vulgaris L.) is one of the important edible beans in the world, which is rich in protein, starch, minerals and vitamins. Common bean, as the main grain crop in my country, has a wide range of planting areas, but it is often harmed by bean anthracnose in production. It is especially serious in the cool and humid environment, the yield is reduced by 30% to 90%, and the plants wilt and die in popular years, which seriously affects the quality and yield of common kidney beans. So far, 20 anthracnose genes or QTLs have been mapped, but there is a lack of molecular markers that can be effectively applied in disease resistance breeding. Therefore, it is very important to discover disease resistance resources, develop d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王述民陈明丽武晶王兰芬朱振东
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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