New wheat stripe rust resisting polygene-polymerized flower-nurture breeding method

Abstract

The invention discloses a new wheat stripe rust resisting polygene-polymerized flower-nurture breeding method. Wheat-breeding parent materials with excellent agronomic characters are selected; 3 majormicro-species of puccinia striiformis, which are recently popular, are used to evaluate stripe rust resistance of the wheat-breeding parent materials at seedling stage and adult stage; 3 wheat-breeding parent materials, which have high-resistance immunity to major popular individual micro-species of various puccinia striiformis, are sieved; the 3 wheat-breeding parent materials are hybridized andduplex hybridized to obtain duplex hybridized polygene-polymerized subsequent generation; the duplex hybridized subsequent generation is then cultured and wheat anther tissue is inoculated to one-step seeding culture medium of wheat anther, thereby obtaining complete pollen plant; chromosome doubling liquid with colchicines and dimethyl sulfoxide is injected to tillering node, thereby carrying out pollen plant chromosome doubling to obtain doubly purified individuals of doubled haploid which can be self-hybridized to form a stable doubled haploid system; and stripe rust resisting individual micro-species validation is carried out on the doubled haploid system, so as to obtain a new stripe rust resisting type or species system which is polymerized with 3 stripe rust resisting genes.

Description

technical field [0001] The invention belongs to wheat biotechnology breeding in food crops, and further relates to a new flower breeding method for anti-stripe rust multi-gene aggregation. Background technique [0002] Wheat is the cereal crop with the largest sowing area, total output and largest trade volume in the world. In my country, wheat is the second largest food crop after rice, and its production status plays a decisive role in my country's national economy. Stripe rust is one of the three major diseases in wheat production. In my country, there have been seven large-scale outbreaks and several medium and small-scale epidemics. Among them, there were four large-scale outbreaks in 1950, 1964, 1990 and 2002. , respectively reduced my country's wheat production by 6 billion kilograms, 3.2 billion kilograms, 2.65 billion kilograms and 1 billion kilograms, causing heavy losses. In the prevention and control of stripe rust, the application of disease-resistant varieties ...

AI Technical Summary

Problems solved by technology

The occurrence and prevalence of wheat stripe rust generally occurs in a certain period of time when multiple races are prevalent at the same time. Although the use of single-gene resistant varieties can obtain good disease resistance results in a certain period, the large-scale planting of single-resistant varieties also affects stripe rust. Physiological races have caused huge selection pressure, accelerated the mutation process of pathogenic races, and there are certain production risks. Historically, the single use of Bima No. 1, Luo Lei varieties, Fan 6, and Mianyang series of stripe rust-resistant varieties has caused After several large and medium-sized stripe rust epidemics in the 1950s, the lessons that caused huge losses to wheat production are profound, and the resistance of slow stripe rust is greatly affected by the environment, and the resistance is unstable.

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