Molecular breeding method for improving cold resistance of rice

A technology of molecular breeding and cold tolerance, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of lack of cold tolerance QTL molecular breeding, QTL identification inaccuracy, etc., to achieve cold tolerance enhancement, identification and Select effects for simplicity, efficiency and accuracy

Inactive Publication Date: 2016-04-13
RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

Although a large number of cold tolerance QTLs have been identified, there is no report on the molecular breeding of cold tolerance QTLs
The diffic

Method used

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  • Molecular breeding method for improving cold resistance of rice
  • Molecular breeding method for improving cold resistance of rice
  • Molecular breeding method for improving cold resistance of rice

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Embodiment 1

[0029] Embodiment 1 A kind of breeding method that improves rice cold tolerance

[0030] This embodiment is a breeding method for improving the seedling cold tolerance of the rice variety "Huajingxian 74", comprising the following specific steps:

[0031] 1. Evaluation of variety cold tolerance

[0032] Through three evaluations on the cold tolerance of 26 rice varieties / lines at the seedling stage, it was found that the indica rice variety "Huajingxian 74" approved by Guangdong Province had poor cold tolerance at the seedling stage, and the average survival rate after cold treatment was 26.63%. The japonica rice variety "Nanyangzhan" has strong cold tolerance at the seedling stage, and the average survival rate after cold treatment is 89.81%. The results are shown in figure 1 shown.

[0033] Among them, the cold tolerance evaluation method at the seedling stage is as follows: select 100 plump seeds for each line, and treat them at a high temperature of 49.0°C for 96 hours t...

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Abstract

The invention discloses a molecular breeding method for improving cold resistance of rice. The method comprises the steps that a single chromosome segment substitution line is established by taking a rice line which is weak in cold resistance as a recurrent parent and taking a rice line which is strong in cold resistance as a cool-resistant gene donor; multi-environment multi-repeat cold resistance detection at an object growth development stage is conducted, and the single chromosome segment substitution line with stable cold-resisting QTL is screened; hybridization is conducted, molecular markers closely linked with the cold-resisting QTL are utilized for assisting in selection, and a polymerization line with different gene locus cold-resisting QTL is obtained; cold resistance evaluation at the object growth development stage is conducted on the polymerization line, and an excellent line of which the cold resistance is significantly improved is obtained through screening. According to the molecular breeding method, identification and selection on the cold resistance are simple, rapid and economical, the excellent line which is excellent in genetic background and significantly enhanced in cold resistance can be rapidly obtained, and the breeding efficiency and accuracy are improved.

Description

technical field [0001] The invention belongs to the field of rice genetic improvement and biotechnology application, and more specifically, the invention relates to a molecular breeding method for improving cold tolerance of rice. Background technique [0002] Rice is an important food crop. More than half of the world's population uses rice as the main food, and it is planted all over the world. Rice is a temperature-loving crop, and its growth and development are very sensitive to temperature. Chilling damage occurs throughout the growth and development of rice from seed germination to maturity. Chilling injury at the germination stage will lead to delayed germination and reduced germination rate, hindering seedling formation; at the seedling stage, low temperature will delay seedling growth or even rotten seedlings and dead seedlings, resulting in poor colony formation and inconsistent maturity; Chilling damage that occurs during this period will cause poor development ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 杨梯丰刘斌张少红赵均良董景芳
Owner RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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