Process for producing physiologically active protein using genetically modified silkworm

a technology of genetically modified silkworm and protein, which is applied in the field of process for producing physiologically active protein using genetically modified silkworm, can solve the problems of difficult to extract and purify the difficulty of purifying the target protein from the body fluid of the silkworm, so as to achieve the effect of reducing the amount of time and labor required for inoculation of recombinant virus, reducing the amount of time and labor required,
US7659112B2Inactive Publication Date: 2010-02-09NAT INST OF AGROBIOLOGICAL SCI
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Patents(United States)
Current Assignee / Owner
NAT INST OF AGROBIOLOGICAL SCI
Publication Date
2010-02-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3′ terminal portion and the DNA sequence of the 5′ terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.
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Description

TECHNICAL FIELD

[0001] The present invention relates to a process for producing a recombinant cytokine using a silkworm incorporating a cytokine gene in its chromosomes. In addition, the present invention relates to a gene recombinant silkworm having the property of producing a recombinant cytokine in a silk gland or cocoon and silk thread, and a vector for inserting an exogenous gene into silkworm chromosomes for producing the recombinant silkworm. In addition, the present invention also relates to a process for producing exogenous protein using insect cells, insect tissue or insects to which a gene has been inserted using the aforementioned vector. Moreover, the present invention relates to silk thread containing an exogenous protein produced by a recombinant silkworm obtained in the present invention.BACKGROUND ART

[0002] The production of exogenous proteins using gene recombination technology is used in various industries. The hosts used for their production consist mainly of E. col...

Claims

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