Diabetes autoantibody immunoblotting reagent kit

A technology of autoantibodies and western blotting, which is applied in the direction of material analysis, measuring devices, instruments, etc. by observing the influence on chemical indicators, and can solve problems such as large differences in test results

Inactive Publication Date: 2007-09-26
SHENZHEN BLOT BIOTECH
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

T 2 DM is caused by the body’s resistance to insulin and the relative reduction of insulin. Therefore, the detection of autoantibodies in diabetes and the accurate classification of diabetes are the main basis for clinical diabetes prevention and treatment. The currently confirmed autoantibodies in diabetes i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] The components of this kit include: 5 plates of blotted membrane strips placed in the reaction tank, 8 strips per plate, 0.5ml of enzyme-linked reagent, 25ml of chromogenic reagent A, 25ml of chromogenic reagent B, 25ml of stop solution, 25ml of concentrated washing solution, standard area bring. Take 2-5ml of blood from the vein, put it in a test tube and centrifuge to separate the serum after coagulation, store the serum specimen at 2-8°C, and store the specimen that cannot be tested within 24 hours at -20°C, if it is severely hemolyzed, has flocs or mildew A serum sample will affect the test.

[0009] Determination steps:

[0010] 1. Preparation of washing application solution: the concentrated washing solution stored at 2-8°C will crystallize, dilute the whole bottle (25ml) of concentrated washing solution to 500ml with distilled water or deionized water, put it into the reagent bottle, and mark on the label The preparation time is specified, stored at 2-8°C, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention discloses one diabetes disease self antigen print agent case, whose islets of Langerhans cell extracts mixture protein composed of Langerhas element, glutamic acid decarboxylase and Langerhas cell; using protein print technique through SDS-polyacrylamide gel electrophoresis ranked by molecule size then into print film; its film bar comprises molecule of each Langerhans self antigen element to be put into reaction tank and serum; if it has antigen, then processing antigen combination and then adding enzyme cross and development agent in the zone; comparing with standard to judge whether serum has the antigen etc.

Description

technical field [0001] The invention relates to a diabetic autoantibody immunoblotting kit. Background technique [0002] Diabetes mellitus is a general term for a group of hyperglycemic diseases, which can be divided into type 1 diabetes mellitus (T 1 DM), type 2 diabetes (T 2 DM) and other types. T 1 DM, also known as immune-mediated diabetes, is caused by autoantibodies destroying islet cells and reducing insulin synthesis and secretion. T 2 DM is caused by the body’s resistance to insulin and the relative reduction of insulin. Therefore, the detection of autoantibodies in diabetes and the accurate classification of diabetes are the main basis for clinical diabetes prevention and treatment. The currently confirmed autoantibodies in diabetes include islet cell antibody (ICA) and insulin antibody (IAA). And glutamic acid decarboxylase antibody (GADA), commonly used enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) assay, due to the limitations of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/559G01N21/78
Inventor 马伟民张永顶
Owner SHENZHEN BLOT BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap