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Diabetes autoantibody immunoblotting reagent kit

A technology of autoantibodies and western blotting, which is applied in the direction of material analysis, measuring devices, instruments, etc. by observing the influence on chemical indicators, and can solve problems such as large differences in test results

Inactive Publication Date: 2007-09-26
SHENZHEN BLOT BIOTECH
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  • Summary
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  • Description
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AI Technical Summary

Problems solved by technology

T 2 DM is caused by the body’s resistance to insulin and the relative reduction of insulin. Therefore, the detection of autoantibodies in diabetes and the accurate classification of diabetes are the main basis for clinical diabetes prevention and treatment. The currently confirmed autoantibodies in diabetes include islet cell antibody (ICA) and insulin antibody (IAA). And glutamic acid decarboxylase antibody (GADA), commonly used enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) assay, due to the limitation of detection methods, the detection results in different laboratories are quite different

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0008] The components of this kit include: 5 plates of blotted membrane strips placed in the reaction tank, 8 strips per plate, 0.5ml of enzyme-linked reagent, 25ml of chromogenic reagent A, 25ml of chromogenic reagent B, 25ml of stop solution, 25ml of concentrated washing solution, standard area bring. Take 2-5ml of blood from the vein, put it in a test tube and centrifuge to separate the serum after coagulation, store the serum specimen at 2-8°C, and store the specimen that cannot be tested within 24 hours at -20°C, if it is severely hemolyzed, has flocs or mildew A serum sample will affect the test.

[0009] Determination steps:

[0010] 1. Preparation of washing application solution: the concentrated washing solution stored at 2-8°C will crystallize, dilute the whole bottle (25ml) of concentrated washing solution to 500ml with distilled water or deionized water, put it into the reagent bottle, and mark on the label The preparation time is specified, stored at 2-8°C, and ...

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PUM

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Abstract

This invention discloses one diabetes disease self antigen print agent case, whose islets of Langerhans cell extracts mixture protein composed of Langerhas element, glutamic acid decarboxylase and Langerhas cell; using protein print technique through SDS-polyacrylamide gel electrophoresis ranked by molecule size then into print film; its film bar comprises molecule of each Langerhans self antigen element to be put into reaction tank and serum; if it has antigen, then processing antigen combination and then adding enzyme cross and development agent in the zone; comparing with standard to judge whether serum has the antigen etc.

Description

technical field [0001] The invention relates to a diabetic autoantibody immunoblotting kit. Background technique [0002] Diabetes mellitus is a general term for a group of hyperglycemic diseases, which can be divided into type 1 diabetes mellitus (T 1 DM), type 2 diabetes (T 2 DM) and other types. T 1 DM, also known as immune-mediated diabetes, is caused by autoantibodies destroying islet cells and reducing insulin synthesis and secretion. T 2 DM is caused by the body’s resistance to insulin and the relative reduction of insulin. Therefore, the detection of autoantibodies in diabetes and the accurate classification of diabetes are the main basis for clinical diabetes prevention and treatment. The currently confirmed autoantibodies in diabetes include islet cell antibody (ICA) and insulin antibody (IAA). And glutamic acid decarboxylase antibody (GADA), commonly used enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) assay, due to the limitations of the...

Claims

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Application Information

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IPC IPC(8): G01N33/559G01N21/78
Inventor 马伟民张永顶
Owner SHENZHEN BLOT BIOTECH
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