Kit for testing neutralizing antibody racing ELISA in human and animal rabies
A detection kit and kit technology, applied in the field of biological vaccine preparation, can solve problems such as long time required, poor sensitivity and specificity, large difference in antibody potency, etc., and achieve accurate quantitative determination, good consistency, and time-consuming short effect
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Embodiment 1
[0037] Preparation method of the kit (1)
[0038] 1. Preparation and Coating of Rabies Virus
[0039] Rabies virus was inoculated into BHK-21 monolayer cells, cultured for 3 to 4 days, frozen and thawed twice, cells were lysed, and culture medium was harvested. When coating, dilute the virus with the coating solution to a total protein concentration of 100 μg / ml, add 100 μl to the enzyme-linked plate, and coat overnight at 4°C; rinse three times with PBS-Tween 20 buffer, and wash the virus three times in each well. Add 100 μl of blocking solution (containing 5% skimmed milk powder and 0.01% thimerosal), place at 37°C for 1 hour, discard the blocking solution, seal it in a vacuum-sealed aluminum foil bag, and store at 4°C.
[0040] 2. Preparation of standard serum
[0041] 2.1 Preparation of human standard serum
[0042]Commercially available human rabies vaccine (Dalian Jingang Andy Biological Products Co., Ltd.) was purchased, and the volunteers were immunized three times ...
Embodiment 2
[0067] The preparation method of the kit (2)
[0068] 1. Preparation and Coating of Rabies Virus
[0069] Rabies virus was inoculated into BHK-21 monolayer cells, cultured for 3 to 4 days, frozen and thawed twice, cells were lysed, and culture medium was harvested. When coating, dilute the virus with the coating solution to a total protein concentration of 100 μg / ml, add 100 μl to the enzyme-linked plate, and coat overnight at 4°C; rinse three times with PBS-Tween 20 buffer, and wash the virus three times in each well. Add 100 μl of blocking solution (containing 5% skimmed milk powder and 0.01% thimerosal), place at 37°C for 1 hour, discard the blocking solution, seal it in a vacuum-sealed aluminum foil bag, and store at 4°C.
[0070] 2. Preparation of standard serum
[0071] 2.1 Preparation of human standard serum
[0072] Commercially available human rabies vaccine (Dalian Jingang Andy Biological Products Co., Ltd.) was purchased, and the volunteers were immunized three t...
Embodiment 3
[0098] The preparation method of the kit (3)
[0099] 1. Preparation and Coating of Rabies Virus
[0100] Rabies virus was inoculated into BHK-21 monolayer cells, cultured for 3 to 4 days, frozen and thawed twice, cells were lysed, and culture medium was harvested. When coating, dilute the virus with the coating solution to a total protein concentration of 100 μg / ml, add 100 μl to the enzyme-linked plate, and coat overnight at 4°C; rinse three times with PBS-Tween 20 buffer, and wash the virus three times in each well. Add 100 μl of blocking solution (containing 5% skimmed milk powder and 0.01% thimerosal), place at 37°C for 1 hour, discard the blocking solution, seal it in a vacuum-sealed aluminum foil bag, and store at 4°C.
[0101] 2. Preparation of standard serum
[0102] 2.1 Preparation of human standard serum
[0103] Commercially available human rabies vaccine (Dalian Jingang Andy Biological Products Co., Ltd.) was purchased, and the volunteers were immunized three t...
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