Separation and determination method for mobility of nicotine optical isomers in cigarettes

A technology for optical isomers and determination methods, which is applied in the field of tobacco analysis, can solve the problems of large influence on the stability of the method, insufficient sensitivity, and long analysis time of two-dimensional gas chromatography, so as to solve the interference of complex matrix and quantitative determination accurate effect

Active Publication Date: 2020-08-07
CHINA TOBACCO YUNNAN IND
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  • Abstract
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Problems solved by technology

In the normal phase liquid chromatography system, the samples, reagents, and moisture in the environment have a great influence on the stability of the method; while the analysis time of two-dimensional gas chromatography is too long, the sensitivity is not enough, and it is difficult to detect samples with a relative content of less than 0.5% in tobacco samples

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  • Separation and determination method for mobility of nicotine optical isomers in cigarettes
  • Separation and determination method for mobility of nicotine optical isomers in cigarettes
  • Separation and determination method for mobility of nicotine optical isomers in cigarettes

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Embodiment Construction

[0033] The content of the present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

[0034] (1) Instruments and reagents

[0035] Agilent1260-Agilent1290 two-dimensional liquid chromatograph (Agilent Company of the United States); Milli-Q-Intergral5 ultrapure water instrument (Millipore Company of the United States); SHZ-88 water bath constant temperature oscillator (Jintan Medical Instrument Factory); BT125D electronic balance ( Sensitivity 0.0001g, German Sartorius Scientific Instrument Co., Ltd.); KQ-700DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); centrifuge tube with stopper, 50mL; disposable sterile syringe; 5mL; organic phase filter membrane , 0.22 μm (Shanghai Chuding Analytical Instrument Co., Ltd.).

[0036] Methanol (chromatographically pure, Merck Company of Germany), acetonitrile (chromatographically pure, Merck Company of Germany), triethyl...

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Abstract

The invention discloses a separation and determination method for the mobility of nicotine optical isomers in cigarettes. The method comprises the following steps of: (1) collecting nicotine optical isomers in cigarettes; 2, collecting nicotine optical isomers in cigarette aerosol; (3) carrying out accurate quantitative determination on S-(-)-nicotine and R-(+)-nicotine; and (4) calculating the mobility of the nicotine optical isomers in the cigarettes. According to the separation and determination method, a Trap column is adopted for the first time to replace a quantitative loop, so that a target object cut by a one-dimensional column can be completely collected, and quantitative determination is more accurate; it is found for the first time that the mobility of R-(+)-nicotine to aerosolin traditional cigarettes is obviously higher than that of R-(+)-nicotine to aerosol in heated cigarettes.

Description

technical field [0001] The invention belongs to the field of tobacco analysis, in particular to a method for separating and measuring the mobility of nicotine optical isomers in cigarettes. Background technique [0002] Nicotine is the most abundant alkaloid in tobacco and smoke aerosol, accounting for 90% to 95% of the total alkaloid content. It can act on the central nervous system of the human body and is the main driving force for smokers to smoke. The nicotine molecule contains a chiral center and has two optically active bodies: S-(-)-nicotine and R-(+)-nicotine, which are completely different in aerosol content, metabolic mechanism and physiological characteristics. Nicotine in tobacco mainly exists in the configuration of L-nicotine (S-(-)-nicotine), and L-nicotine accounts for more than 99% of the total nicotine. [0003] The migration rate of nicotine in cigarettes to aerosol has guiding significance for cigarette formulation. There are not many research reports ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/14G01N30/46
CPCG01N30/06G01N30/14G01N30/463G01N2030/062
Inventor 朱瑞芝司晓喜刘志华王凯蒋薇李振杰张凤梅何沛杨继向能军刘春波唐石云
Owner CHINA TOBACCO YUNNAN IND
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