Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of protein to preparation of medicine for preventing trueperella pyogenes infection

A technology of cryptobacillus pyogenes and protein, applied in application, antibacterial drugs, bacterial peptides, etc., can solve the problem of lack of new protective antigens

Active Publication Date: 2020-01-03
CHONGQING ACAD OF ANIMAL SCI
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The identified virulence factors of C. pyogenes include hemolysin (Pyolysin, PLO), adhesion protein collagen-binding protein (collage-binding protein, CbpA), neuraminidases (Nan) NanH and NanP, etc., and PLO is the main one. protective antigens, no new protective antigens have been discovered so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of protein to preparation of medicine for preventing trueperella pyogenes infection
  • Application of protein to preparation of medicine for preventing trueperella pyogenes infection
  • Application of protein to preparation of medicine for preventing trueperella pyogenes infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. Analysis of immunoreactive membrane proteins

[0033] 1. Antiserum preparation of C. pyogenes

[0034] The strain is the Chongqing isolate of Cryptobacterium pyogenes in the patent application number CN201410072433.8 (biological deposit number: CCTCC NO: M2014037, (GenBank NO: CP012649).

[0035]A single colony of C. pyogenes was picked to inoculate TSB medium, and cultured at 37°C for 48 hours. Add 0.2% formaldehyde solution to the culture, inactivate it at 37°C for 24 hours, and shake it 5 times during the period. Mix and emulsify the inactivated culture with Freund's adjuvant to prepare the inactivated antigen. Leg intramuscular injection, 0.2mL / mouse, immunized once every 2 weeks, immunized 3 times in total. Fifteen days after the third immunization, the blood of the mice was collected by enucleating the eyeballs, and centrifuged to collect the antiserum of C. pyogenes after blood coagulation.

[0036] 2. Membrane protein separation and mass spectrometry anal...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of a veterinary biological product, and discloses an antigen protein of trueperella pyogenes, preparation of the antigen protein, application of the antigen proteinto a vaccine, and an antibody detection method. A gene of a highly conservative trueperella pyogenes Fe ABC (ATP Binding Cassette) transporter SBP (Substrate-Binding Protein) is cloned to a pGEX-4T-1plasmid; a BL21(DE3) recombinant strain is built; IPTG is used for induction expression of the recombinant protein SBP (GST-SBP); the soluble expression of the target protein is realized; and the recombinant protein is subjected to affinity purification by a Gluthathione-Sepharose 4B chromatography method. The recombinant SBP (GST-SBP) protein provided by the invention has high reactionogenicity and immunogenicity, and can react with the blood serum of trueperella pyogenes immunized or infected animals (mice and goats); the animals (mice and goats) can be stimulated to generate high-level specific antibodies; and the immune protective rate of the mice is 90 percent. The novel antigen protein is provided for the preparation of medicine for preventing the trueperella pyogenes infection.

Description

technical field [0001] The invention belongs to veterinary biological products, in particular to an antigen protein for preparing and preventing infection of Cryptobacter pyogenes. Background technique [0002] The goat industry has developed rapidly in the Three Gorges reservoir area in recent years, and has become one of the important industries that help farmers get rid of poverty and become rich and the healthy development of rural areas. However, the impact of epidemic diseases on sheep farming in the region is becoming more and more apparent. Among them, suppurative pneumonia is more harmful. Because of its long latent infection period and no obvious clinical symptoms, suppurative lesions cause irreversible damage to lung tissue, which seriously affects the survival rate of lambs. Trueperella pyogenes is one of the important pathogens of goat suppurative pneumonia (Zhang Suhui et al., 2015; Fu Lizhi et al., 2018; Feng Feng, 2010.). Cryptobacterium pyogenes is a Gram-p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/195C12N15/31C12N15/70G01N33/68A61K39/02A61P31/04
CPCC07K14/195C12N15/70G01N33/68A61K39/0208A61P31/04G01N2333/195A61K2039/552Y02A50/30
Inventor 沈克飞张素辉闫志强徐登峰许国洋付利芝
Owner CHONGQING ACAD OF ANIMAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com