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Duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof

A duck tembusu virus and immunotherapy technology, which is applied in the direction of antiviral agents and antibodies, can solve problems such as the research report on the immunotherapy of duck tembusu virus disease, which is suitable for large-scale production, simple and safe equipment good sex effect

Active Publication Date: 2012-11-28
GUANGZHOU GREAT BIOLOGICAL TECH +1
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Problems solved by technology

[0004] So far, there are no domestic and foreign research reports on the immunotherapy of duck Tembusu virus disease

Method used

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  • Duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof
  • Duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof

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specific Embodiment

[0030] Specific embodiment: the preparation method of duck Tembusu virus disease immunotherapy preparation

[0031] The preparation steps of duck Tembusu virus disease immunotherapy preparation are as follows:

[0032] The seed strain used for the antigen of the present invention is Flaviviridae Flavivirus genus Yatambusu virus Guangdong strain (JM), which was sent to the China Center for Type Culture Collection on October 14, 2011 for preservation, and the preservation number is CCTCCV201131.

[0033] 1. Breeding of poisonous species

[0034] Inoculate 11-day-old shelduck duck embryos with the 7th generation allantoic fluid poison of JM strain (code name JMDE7, ELD50 is 10-6.0 / ml) through the allantois, 0.2ml / embryo, culture at 37°C, irradiate embryos twice a day, discard After 24 hours of dead embryos, the dead duck embryos were placed in a 4°C refrigerator, and the dead embryo allantoic fluid (hereinafter referred to as toxic allantoic fluid) was aseptically harvested with...

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Abstract

The invention relates to a duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof, which belong to the field of bio-pharmaceuticals. The preparation method of the DTMUV disease immunotherapy preparation comprises the following steps of: culturing seed viruses for producing a tembusu virus strain on a large scale, and harvesting a virus liquid; deactivating the collected virus liquid, concentrating, adding the deactivated and concentrated virus liquid into an adjuvant, and emulsifying to obtain an immune antigen; immunizing laying ducks by using the immune antigen, randomly collecting blood and separating serum after last immunization, and measuring the valence of an anti-DTMUV antigen through an Agar Gel Precipiti (AGP) test till the valance of the antigen is qualified; and collecting eggs laid by an immunized qualified duck group, and preparing into a high-immunity egg yolk antibody with the conventional method. The DTMUV disease immunotherapy preparation contains a DTMUV high-immunity egg yolk antibody. The DTMUV disease immunotherapy preparation prepared with the method has the advantages of good treatment effect, safety, reliability and low cost, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to an immunotherapy preparation for duck Tembusu virus disease and a preparation method thereof. Background technique [0002] Duck Tembusu virus disease, also known as "duck yellow virus disease", "duck hemorrhagic oophoritis", "laying duck yellow virus disease" and "egg production drop syndrome caused by a new type of flavivirus BYD", it was reported in 2010 A new duck disease was first discovered in Zhejiang, Fujian, Shandong, Guangdong and other places in China. It mainly occurs in laying ducks, but also in breeding geese and meat ducks. When the disease occurs in laying ducks, the clinical manifestations are unstable limbs, difficulty in walking, decreased feed intake, a sudden decrease in egg production, and a large egg loss rate. The egg loss exceeds 90% within 5 days or no production. Death within a few days, the mortality rate is 5% to 10%. Necropsy lesions were ovarian he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61P31/14
Inventor 魏平华胡奇林程怀灵孙敏华魏苏红董嘉文邱志欣李林林黄志松袁建丰
Owner GUANGZHOU GREAT BIOLOGICAL TECH
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