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Use of immunological stimulant compound(ISCOMs)in fish immunity by oral administration and dipping bath method

An immune stimulation and compound technology, applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems of difficult preparation of vaccines

Inactive Publication Date: 2006-12-20
BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artemia-carried vaccines are also difficult to prepare in an industrialized and standardized manner

Method used

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  • Use of immunological stimulant compound(ISCOMs)in fish immunity by oral administration and dipping bath method
  • Use of immunological stimulant compound(ISCOMs)in fish immunity by oral administration and dipping bath method
  • Use of immunological stimulant compound(ISCOMs)in fish immunity by oral administration and dipping bath method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Immune-stimulating complexes (ISCOMs) made of Vibrio vulnificus bacterium protein for oral immunization of eel

[0101] 1. Materials and methods

[0102] 1.1 Materials

[0103] The strain FJ03-X2 used to produce this product was isolated from the blood of European eels suffering from vibriosis in Fujian in mid-June 2003, and was identified as biological type 1 Vibrio vulnificus by strict biochemical identification and molecular biology methods. LD of 20-30g / tail European eel 50 About 7.1×10 3 CFU / tail. The strain was isolated, identified and preserved by the Institute of Biotechnology, Fujian Academy of Agricultural Sciences. The main reagent QuilA was purchased from Accurate Chemical & Scientific Corporation; Mega-10 was purchased from Huamei Bioengineering Company; the tested eels were from Changle Hailin Eel Farm in Fujian Province, and the specification was 50g per fish.

[0104] TSB Medium Formula

[0105] Soytone 3g, Tryptone 17g, NaCl 15g, K 2 HPO 4 2.5g...

Embodiment 2

[0122] Example 2 The application of immunostimulatory complexes (ISCOMs) made of seawater pathogenic bacteria bacterial protein in oral immunization of seawater fish

[0123] 1. Materials and methods

[0124] 1.1 Materials

[0125] The bacteria used in the manufacture of this product are Vibrio parahaemolyticus (wVp), Vibrio alginolyticus (Val33) and Vibrio harveyi (Vha), all of which are kept by the Institute of Biotechnology, Fujian Academy of Agricultural Sciences , identification and supply. The main reagent QuilA was purchased from Accurate Chemical & Scientific Corporation; Mega-10 was purchased from Huamei Bioengineering Company; the large yellow croaker and red sea bream used in the test were from Xiapu County, Fujian Province.

[0126] 1% NaCl Nutrient Broth Formula

[0127] Peptone 10g, beef extract powder 3g, NaCl 10g, add double distilled water to 1000ml, pH 7.3±0.2, autoclave at 121°C for 20min.

[0128] 1% NaCl Nutrient Agar Medium Formula

[0129] Peptone 1...

Embodiment 3

[0144] Example 3: Application of Immunostimulatory Complexes (ISCOMs) made by Aeromonas hydrophila on eel oral immunization

[0145] 1. Materials and methods

[0146] 1.1 Materials

[0147] The strain TPS30 used to produce this product was provided by Qian Dong, Zhejiang Freshwater Research Institute. The ZN1 strain was isolated from a European eel suffering from Aeromonas hydrophila sepsis in Zhouning County, Fujian Province in 2004 by the Institute of Biotechnology, Fujian Academy of Agricultural Sciences. The above strains are kept, identified and supplied by the Institute of Biotechnology, Fujian Academy of Agricultural Sciences. The main reagent QuilA was purchased from Accurate Chemical & Scientific Corporation; Mega-10 was purchased from Huamei Bioengineering Company; the tested eel was from Changle City, Fujian Province.

[0148] TSB Medium Formula

[0149] Soytone 3g, Tryptone 17g, NaCl 15g, K 2 HPO 4 2.5g, glucose 0.5g, add double distilled water to 1000ml, aut...

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PUM

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Abstract

The invention relates to a bath or oral application of immune activate compound in the fish immunity, wherein the invention has the advantages that: said compound ISCOMs is used to bath immunity to improve the antibody level and improve the increment transformation ability of T-lymphocyte, to induce the immune protection; when in the same dosage, the antibody level is higher than non-adjuvant group and the ISM1312 group; and the ISCOMs used in oral can improve the antibody level and improve the increment transformation ability of T-lymphocyte, to induce the immune protection.

Description

technical field [0001] The invention relates to the application of an immunostimulating compound in fish immunity, especially the application in fish immunity by oral administration and bathing. Background technique [0002] The immunization methods of aquatic animal vaccines mainly include injection, bathing and oral administration. Although injection immunization can obtain a good immune effect, it is not suitable for large-scale farming because it needs to be carried out on an individual basis. application. [0003] Aquatic animals, especially fish, have a well-developed mucosal system consisting of body surface, gastrointestinal tract, and gills. In addition to participating in non-specific immunity, the mucosal system can also mediate specific immune protection. , Traditional vaccines are affected by various enzymes in the intestines, pepsin and body surface mucosa, and the effective antigens are seriously lost during oral or bath immunization, and the penetration is n...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P37/04
Inventor 龚晖林天龙许斌福
Owner BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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