ELISA kit of IBDV antibodies, test method and effective antibody titer determination method

Abstract

The invention discloses an ELISA kit of IBDV antibodies, a test method and an effective antibody titer determination method. The ELISA kit comprises (1) an enzyme-labeled board coated with IBDV antigens and (2) goat anti-chicken Ig Y diluted according to the ratio of 1:3,000 and marked with HRP, wherein the enzyme-labeled board coated with the IBDV antigens is made by diluting the concentration ofthe IBDV antigens to 10ng / pore and then coating corresponding pores in a reaction board with diluent, the IBDV antigens are rVP2 proteins prepared through a baculovirus expression system, and a detection result is judged according to an S / P value obtained through an ELISA detection method. When the detection result is positive according to the S / P value obtained through the ELISA detection method, it is indicated that effective antibodies are in a high level and can neutralize IBDVs, and therefore it is needless to perform vaccine immunization again; and when the detection result is negativeaccording to the S / P value, it is indicated that the antibodies are in a low level without protective force and are prone to being infected by viruses, and therefore it is needed to perform vaccine immunization again. As a result, the established ELISA kit has a high antibody positivity detection rate and can guide vaccine immunization on production to reduce economic losses.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to an ELISA test box and a test method for IBDV antibodies, and a method for determining effective antibody titers. Background technique [0002] IBDV causes severe economic losses to poultry farming. VP2 protein is the main antigen protein of IBDV, the main target of IBDV molecular mechanism research and veterinary drug research, and also one of the important indicators of IBDV virus and antibody detection. Immunological diagnostic techniques are widely used in IBDV research, investigation and vaccine immune effect evaluation. At present, domestic IBDV detection-related products include IBDV colloidal gold antigen detection test strips, IBDV antibody ELISA detection kits, etc. Colloidal gold test strips can only be used for virus detection, not for immune evaluation. IDEXX antibody detection kits rely on imports, the price is too expensive, and the product cannot be disassembled for m...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide an ELISA kit, a test method and a method for determining the effective antibody titer of IBDV antibodies, to solve the complicated and expensive technical problems of the prior art test methods; and to solve the problem that the prior art cannot be used for immune evaluation question

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