Competitive ELISA (Enzyme-Linked Immuno Sorbent Assay) kit for detecting antibody of African swine fever virus and application thereof

An African swine fever virus and kit technology, which is applied to an ELISA kit for detecting antibodies in animal quarantine. For the detection of African swine fever virus antibody detection kits, it can solve problems such as loss of activated caspase and achieve safety. Good, reliable effect, stable production effect
CN101833005AInactive Publication Date: 2010-09-15ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2010-09-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a competitive ELISA (Enzyme-Linked Immuno Sorbent Assay)kit for detecting an antibody of an African swine fever virus and application thereof, belonging to the technical field of organisms. The kit is used for detecting an antibody of the African swine fever virus in pig serum by adopting prokaryotically expressed recombinant P54 protein as an envelope antigen according toa competitive ELISA principle. The envelope antigen in a 96 pore plate in the kit is prokaryotically expressed recombinant P54 protein and has favorable antigenicity. The enzyme-linked immuno kit provided by the invention comprises the P54 protein enveloped 96 pore plate, positive control, negative control, a horseradish peroxidase marked monoclonal antibody, a concentrated cleaning solution, serum diluent, a TMB substrate and a stopping solution. The kit can be used for screening samples in bulk, main reagents in the kit are provided in a working solution way, and the use is convenient.
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Description

technical field

[0001] The invention is a detection kit for detecting African swine fever virus (ASFV) antibody, which belongs to the field of biotechnology. Specifically, the present invention relates to an ELISA kit for detecting antibodies in animal quarantine and use thereof. Background technique

[0002] African swine fever (ASF) is an acute, febrile and highly contagious infectious disease of pigs caused by African swine fever virus (ASFV). It is characterized by a short course of disease and a high mortality rate, which can be as high as 100%. The clinical symptoms and pathological changes are similar to acute swine fever. It is easy to be misdiagnosed at the time of diagnosis, and manifests as high fever, skin congestion and cyanosis, miscarriage, edema and organ hemorrhage. (William, Hess Adv. African swine fever: a reassessment [J]. Vet Sci Comp Med, 1981, 25: 39-69) The World Organization for Animals (OIE) lists it as a Class A disease, and my country defines it ...

Claims

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