A-type foot-and-mouth disease targeting composite epitope protein mediated by pig chemotactic factors and vaccine

Abstract

The invention provides A-type foot-and-mouth disease targeting composite epitope protein mediated by pig chemotactic factors.The amino acid sequence of the protein is as shown in SEQ ID No.2 in a sequence list.The invention further provides a vaccine with the pig A-type foot-and-mouth disease targeting composite epitope protein.An FMDV specific antibody can be detected one week after an animal is immunized by the vaccine, and the valence of the antibody is continuously improved and reaches the highest four weeks after immunization.After 1000 pigs of the HNXX strain of the A / SEA / 97 series of the pig media infective dose (PID50) adapt to toxin attack, the pigs in the vaccine immunity group can all completely resist strong toxin attack, and no pigs present clinical symptoms within 10 days after toxin attack.It is shown that after an A8 antigen targets an XCR1 receptor through XCL1 and dendritic cells are activated by CpG, the body fluid immunity and cell immunity level of the antigen can be remarkably improved, and thus the vaccine is an effective vaccine with the A-type foot-and-mouth disease targeting composite epitope protein mediated by pig chemotactic factors.

Description

technical field [0001] The invention belongs to the technical field of foot-and-mouth disease immunity, and in particular relates to a type A foot-and-mouth disease targeting complex epitope protein and vaccine mediated by porcine chemokines. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious infectious disease caused by foot-and-mouth disease virus (FMDV). There are more than 70 susceptible species of other domestic and wild artiodactyls. Huge losses have been caused to the development of animal husbandry. FMDV not only has a wide host range, spreads rapidly, and mutates quickly, but also has many serotypes. Foot-and-mouth disease virus is divided into seven serotypes: A, O, C, Asia1, SAT1, SAT2, and SAT3. There is no cross-protection between the serotypes, and the antigens of the subtypes and isolates of different serotypes vary greatly. At present, the prevention and control of foot-and-mouth disease is mainly based on im...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of existing epitope protein vaccines, and provide a type A foot-and-mouth disease targeting complex epitope protein and vaccine mediated by porcine chemokines

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