A Schistosoma japonicum vaccine expression vector secreted and expressed by attenuated Salmonella and its application
A Salmonella, secretion and expression technology, applied in the field of Schistosoma japonicum vaccine expression vector, can solve the problems of limiting the effective presentation of antigens, and achieve the effects of high-efficiency secretion, high-efficiency expression and low cost
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[0031] Taking the Schistosoma japonicum bivalent antigen sj23LHDGST (the large hydrophilic region of the sj23 molecule and the fusion protein of GST) as the delivery antigen as an example:
[0032] 1. Construction of secretory expressed vaccine vectors:
[0033]Antigen molecule sj23LHD-GST is secreted by Salmonella type Ⅲ secretion system and hemolysin secretion system respectively.
[0034] a) Construction of type III secretion expression vector: through the N-terminal 1-104 amino acids of type III secretion effector molecule sopE (sopE 1-104 ) is coupled with the antigen molecule sj23LHD to realize its secretion; the Escherichia coli nitrate reductase (nirB) promoter is used to express the type III secreted antigen. The nirB promoter was obtained by PCR method: the primer sequences were: P1: 5'-cccctcgagggttaccggcccgatcg-3'; P2: 5'-cccggatccaccgcctaccttaacgattc-3'; 50 ng of Escherichia coli genomic DNA was used as a template. The obtained fragments had XhoI and BamHI restr...
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