49results about How to "Reduce recycling steps" patented technology

The invention belongs to the technical field of pigment extraction, and particularly relates to a method for extracting haematochrome from mulberry red. The method comprises the following steps of (1) preprocessing; (2) preparing a separating medium through a freezing wall-breaking method; (3) extracting an extracting solution through distilled water; (4) degumming through an enzymic method; and (5) recycling haematochrome through distilling. The method has the beneficial effects that natural biological activity pigment component in the mulberry pulp is furthest reserved due to the freezing wall-breaking method, and the natural edible haematochrome prepared by the method has strong water solubility, is amaranth, reserves maximum nutritional ingredients of the mulberry, and can be used in food, beverage, cosmetics and the like.

The invention relates to a modified cloning vector and an application thereof. The modified cloning vector is an annular cloning vector, and is obtained by replacing multiple cloning sites of a pUC57 vector by employing three flush end cloning sites of EcoRV, SmaI and StuI and removing an NdeI enzyme digestion site at the LacZ downstream in the pUC57 vector, and the modified cloning vector only comprises three cloning sites of EcoRV, SmaI and StuI. The vector can completely replace any one cloning vector and is convenient to use and is wide in application.

The invention discloses a method for improving the dispersibility of insoluble sulfur. The method comprises the following steps: selecting an organic matter having the melt point or the softening point being 40-100 DEG C as a dispersible material; heating to enable the dispersible material to be kept a molten state before filling oil to the dispersible material; then, uniformly spraying the molten dispersible material onto the surface of the insoluble sulfur in a mist form; carrying out filled sealing on micropores on the surface of the insoluble sulfur; carrying out oil filling after the dispersible material and the insoluble sulfur are sufficiently mixed. According to the method disclosed by the invention, the use of an organic solvent can be avoided by defining and improving the melt point or the softening point of the dispersible material and adding the dispersible material at a molten state in a spraying manner before oil filling rather than an organic solvent dissolving manner, and recovery steps of the organic solvent are also reduced, so that the consumption of resources and energy sources is reduced; meanwhile, the method is simple and feasible, and is beneficial for improving the production efficiency.

The invention discloses a method for electrochemically synthesizing 3-alkylselenyl-4-aminocoumarin compounds. The method is to use the solution containing 4-anilinocoumarin compound, dialkyl diselenide and bromine salt as the electrolyte, place a nickel anode and a graphite rod cathode in the electrolyte, feed in a direct current, and carry out electrolysis. 3-Alkylselenyl-4-anilinocoumarin compound can be obtained through chemical reaction; the method has the advantages of mild reaction conditions, simple operation, environmental protection, easy availability of raw materials, excellent compatibility of substrate functional groups, and high reaction yield.

The invention discloses a method for preparing immobilized cells for producing mannose and a method for producing mannose. Wherein, when the immobilized Bacillus subtilis of the present invention continuously catalyzes the reaction, the initial product yield can reach up to 65%, and after 25 batches of continuous catalysis, the product yield can still maintain 45%. When the immobilized Escherichia coli of the present invention continuously catalyzes the reaction, the initial product yield can reach up to 65%, and after 25 batches of continuous catalysis, the product yield can still maintain 44%. The invention simplifies the separation and purification steps of the enzyme, improves the recycling rate of the enzyme, and realizes the recycling of the enzyme. The method of the invention has the advantages of easy product separation, simple production process, low cost, etc., and provides reference for industrial application.

The invention relates to a process for synthesizing p-tert-butylbenzoic acid. The process comprises the following steps of: adding p-tert-butyltoluene and cobalt acetate into a reaction kettle, introducing oxygen-containing gas, triggering at the temperature of between 150 and 155 DEG C, performing oxidation reaction for 0.5 to 1 hour, and continuously reacting at the temperature of between 135 and 145 DEG C for more than 5 hours to obtain a p-tert-butylbenzoic acid crude product; cooling the p-tert-butylbenzoic acid crude product to crystallize, performing centrifuge dripping, and striking and washing by using p-tert-butyltoluene to obtain a centrifugation crude product; and adding the centrifugation crude product into a dissolution kettle, adding methylbenzene, raising temperature and dissolving, filtering when a mixture is hot to remove impurities, adding water to wash, transferring materials to a recrystallization kettle, cooling to crystallize, centrifuging, striking and washing by using methylbenzene, and drying to obtain a p-tert-butylbenzoic acid finished product. The prepared p-tert-butylbenzoic acid is white crystals and does not contain mechanical impurities; and the melting point is 164.0 to 167.0 DEG C, the acid value is 312 to 316mgKOH / g, high performance liquid chromatography (HPLC) purity is more than or equal to 99.2 percent, moisture is less than or equal to 0.08 percent, and methylbenzene is less than or equal to 0.05 percent.