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Preparation method and application of immobilized cells for tagatose production

A technology for immobilizing cells and tagatose, applied in the production and preparation of tagatose, and in the field of bioengineering, can solve the problems of uneven particle size of immobilized enzymes, loss of immobilization efficiency of enzymes, cumbersome production steps, etc., and achieve simple immobilization Chemical process, which is beneficial to separation and purification, and simplifies the effect of production and preparation process

Active Publication Date: 2021-08-13
天津怡和生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems existing in the existing methods for preparing tagatose from immobilized cells, such as cumbersome production steps of enzymes, loss of enzymes caused by permeabilization treatment and reduction of immobilization efficiency, uneven particle size of immobilized enzymes prepared, etc. , the purpose of the present invention is to provide a method for producing tagatose by immobilized cells, simplify the production steps of enzymes in the production process of tagatose, simplify the separation and purification of products and enzymes in the production process of tagatose, and realize the circulation of multiple enzymes use, reduce the production cost of tagatose, and realize the industrialized production of tagatose

Method used

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  • Preparation method and application of immobilized cells for tagatose production
  • Preparation method and application of immobilized cells for tagatose production
  • Preparation method and application of immobilized cells for tagatose production

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1: Preparation of Bacillus Bacillus Bacillus

[0036] Picking with heat-resistant α-glucan phosphorylase gene, expressing heat-resistant glucose phosphate amzyase gene, expressing heterosphosphoric acid resistance of heat-resistant glucose phosphate, expression of heat-resistant 6-phosphate straw glycetin Gene, expressed by Bacillus recombinant, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Selected SCK6, see CN112342179B), and inoculated in LB medium, 37 ° C oscillated overnight culture. The culture was transferred to the lb medium at 1% inoculation, and the bunch of Bacillus fermentation liquid expressed by heat-resistant alpha-glucan phosphatase was obtained at 37 ° C to expressed heat-resistant glucose phosphate. Bacillus fermentation broth, Bacillus fermentation broth, expression of hemorrhosphate, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Expression, Bacillus, Expressin...

Embodiment 2

[0037] Example 2: Fixed brown sporosine production Taggosa

[0038] The Bacillus fermentation of Bacillus acid-resistant alpha-glucan phosphatase prepared according to OD600 ratios 1: 1: 1: 1: 1, Bacillus Bacillus expressing heat-resistant glucose phosphatase Bacillus fermentation of Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus fermentation, expressing heat-resistant 6-phosphate tartlastinase Bacillus fermentation broth was mixed such that OD 600 = 100, 1% w / v montmorillonite was added to the bacterial suspension, and stir evenly. Subsequently, a 0.5% W / V molecular weight of 10,000 polyethyleneimine solution was flocculated under room temperature conditions. Then, 2% V / V glutaraldehyde aqueous solution was added to each other under room temperature. After the vacuum was filtered, the filter cake was obtained, and the filter cake was crimped into a long strip shape of 1.0 mm particle diameter, and then ...

Embodiment 3

[0040] Example 3: Comparative Tagguria

[0041] The Bacillus fermentation of Bacillus acid-resistant alpha-glucan phosphatase prepared according to OD600 ratios 1: 1: 1: 1: 1, Bacillus Bacillus expressing heat-resistant glucose phosphatase Bacillus fermentation of Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus, Bacillus fermentation, expressing heat-resistant 6-phosphate tartlastinase Bacillus fermentation broth was mixed, allowing OD 600 = 100, and 5% w / v celite was added to the bacterial suspension, and stir evenly. Subsequently, a 0.1% W / V molecular weight of 70000 was added to flocculate under room temperature conditions. Then, a 1% V / V glutaraldehyde solution was added to crosslink 2 h under room temperature conditions. After the vacuum was filtered, the filter cake was obtained, and the filter cake was extracted with a rotary granulator, and then the length of the particle diameter was concentrated, and then...

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Abstract

The invention discloses a preparation method of immobilized cells for tagatose production and a method for producing tagatose by using the immobilized cells. When immobilized bacillus subtilis is subjected to continuous catalytic reaction, the yield of an initial product can reach 75% at most, and after continuous catalysis is carried out for 65 batches, the yield of the product can still be maintained to be 61%. When immobilized Escherichia coli is subjected to continuous catalytic reaction, the yield of an initial product can be up to 74% at most, and after continuous catalysis is carried out for 65 batches, the yield of the product can still be maintained to be 60%. The separation and purification steps of the enzyme required in tagatose production are simplified, the recycling rate of the enzyme is improved, and the recycling of the enzyme is realized. The method has the advantages of easiness in product separation, simple production process, low cost and the like.

Description

Technical field [0001] The present invention relates to the field of bioengineering, in particular to the production of production and preparation of Taggosa. Background technique [0002] Taggosa is a naturally occurring monosaccharide, and it is a hemonctose form, a differentially isomer of fructose. The sweetness of Taggosa is similar to sucrose, providing very fresh and pure sweetness, but only one-third of the caloric sucrose is called low-calorie sweeteners. Studies have shown that Taggos have low calories, low-sugar index, anti-caries, anti-oxidation, probiotics, improve intestinal functions, immune regulation, drug precursors, etc., can be widely used in food, beverages, medicine, Health and other areas, huge economic value (Oh DK: tagatose: Properties, Applications, And Biotechnology Processes. App. Microbiol. Biotechnol. 2007, 76: 1-8). [0003] Tianjin Industrial Biotechnology Research, Suitable for cheap corn starch, maltodextrin, sucrose, etc., successfully establish...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/089C12N11/087C12N11/082C12P19/02C12R1/19C12R1/125
CPCC12N11/14C12N11/10C12N11/089C12N11/087C12N11/082C12P19/02C12N11/08C12Y204/01001C12Y504/02002C12Y503/01009C12N11/06C12N11/098C12N1/20
Inventor 马延和石婷韩平平
Owner 天津怡和生物科技有限责任公司
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