A preparation method and application of immobilized cells for mannose production

A technology for immobilizing cells and mannose is applied in the production and preparation of mannose and in the fields of bioengineering, and can solve the problems of high production cost of mannose, low enzyme recovery rate, cumbersome production steps, etc. Conducive to the effect of separation and purification, simplifying the production and preparation process
CN113249372BActive Publication Date: 2021-10-08天津怡和生物科技有限责任公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
天津怡和生物科技有限责任公司
Publication Date
2021-10-08

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Abstract

The invention discloses a method for preparing immobilized cells for producing mannose and a method for producing mannose. Wherein, when the immobilized Bacillus subtilis of the present invention continuously catalyzes the reaction, the initial product yield can reach up to 65%, and after 25 batches of continuous catalysis, the product yield can still maintain 45%. When the immobilized Escherichia coli of the present invention continuously catalyzes the reaction, the initial product yield can reach up to 65%, and after 25 batches of continuous catalysis, the product yield can still maintain 44%. The invention simplifies the separation and purification steps of the enzyme, improves the recycling rate of the enzyme, and realizes the recycling of the enzyme. The method of the invention has the advantages of easy product separation, simple production process, low cost, etc., and provides reference for industrial application.
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Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to the field of production and preparation of mannose. Background technique

[0002] Mannose is a six-carbon sugar that is an isomer of glucose. Mannose has the functions of regulating immunity, resisting bacterial and viral infection, promoting glycoprotein synthesis in the body, and anticancer. Therefore, mannose is widely used in the fields of biology and medicine. Mannose can not only be used as a raw material for the synthesis of some pharmaceutical chemical raw materials and important sugar drug precursors, but also can be used as a sweetener for food and beverages. In recent years, the demand for mannose in the fields of functional food, feed additive and medicine has increased significantly, and it has huge economic development value.

[0003] The main methods of industrial production of mannose are extraction and isomerization. The extraction method mainly uses c...

Claims

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