74 results about "Aminocoumarins" patented technology

The invention provides a fluorescent detection method for quantitatively detecting ferric ions on the basis of a coumarin derivative (molecular formula: C14H5NO3), and the coumarin derivative 7-lignocaine coumarin-4-formaldehyde is synthesized according to documents. The method comprises the steps: coordinating Fe<3+> and two carbonyls in the 7-lignocaine coumarin-4-formaldehyde structure in a HEPES buffer solution in a pH value of 7.0, and detecting the ferric ions through the fluorescence quenching. The detection method is high in sensitivity and selectivity for the display of the ferric ions, the detection reagent is cheap, the detection process is simple, sensitive and rapid, and the detection result is accurate. In addition, a confocal fluorescence image further proves that a probe can be used for recognizing the Fe<3+> in a living cell.

This invention features new compositions and methods that are useful in treating a host with a Gram-negative bacterial infection. Combination therapies comprising an aminocoumarin compound and a polymyxin compound are disclosed, including certain combinations that exhibit synergistic effects. Furthermore, aminocoumarin compounds are described having altered inhibition of DNA gyrase in Gram-negative bacteria and / or the ability to target the transport proteins responsible for assembling lipopolysaccharide in the outer membrane of Gram-negative bacteria.

The invention provides a fluorescent probe based on a chalcone structure, a preparation method and its application in detecting hydrazine. The probe specifically involved is 3-(3'-(2'-chloro-7'-(diethylamino)quinolyl)allyl)-7-diethylaminocoumarin (QCT-Cl). The probe QCT‑Cl is prepared from 2‑chloro‑7‑diethylaminoquinoline formaldehyde and 7‑diethylaminocoumarin‑3‑ethanone through condensation reaction, with mild synthesis conditions and simple purification . In the present invention, QCT-Cl is a fluorescence-enhanced fluorescent probe. After hydrazine interacts with it, its solution can be seen by the naked eye under an ultraviolet lamp from weak red to bright blue. It has high selectivity, strong anti-interference, and sensitivity. High stability, the lowest detection limit of hydrazine is 9.46×10 ‑8 mol / L, it can be used as a fluorescent probe for the qualitative and quantitative detection of hydrazine, and is widely used in the detection of hydrazine in the environmental and biological fields.

Selective enrichment media and methods for selectively growing and detecting Salmonella spp. and / or Shiga toxin-producing E. coli. The media may comprise a carbon and nitrogen source, an inorganic salt, a fermentable sugar, one or more selective agents, and an efflux pump inhibitor. Various selective agents include sulfa drugs, surfactants, aminocoumarins, cycloheximide, supravital stains, ascorbic acid, bromobenzoic acid, myricetin, nitrofurantoin, rifamycins, polyketides, and oxazolidinones. Various efflux pump inhibitors include arylpiperazines, such as 1-(1-naphthylmethyl)piperazine, and quinoline derivatives, such as 4-chloroquinoline. Methods of selectively growing and detecting Salmonella and / or Shiga toxin-producing E. coli are provided.

The invention belongs to the technical field of fluorescence detection, and discloses a bifunctional fluorescent probe for detecting hypochlorite ions and / or singlet oxygen in the mitochondria of cells and its preparation method and application. By preparing 7-(diethylamino) After a series of steps such as coumarin and 7-(diethylamino)coumarin aldehyde, a bifunctional fluorescent probe for detecting hypochlorite ion and / or singlet oxygen in the mitochondria of cells is obtained. After the improvement of the synthesis method of the present invention, the occurrence of side reactions can be effectively prevented, and impurities that are difficult to separate during the reaction process can be greatly reduced, thereby obtaining a high-purity target product. The bifunctional fluorescent probe prepared by the present invention can not only detect chlorate ion or singlet oxygen alone, but also distinguish the levels of hypochlorite ion and singlet oxygen at the same time, and can also be used for endogenous hypochlorite ion and singlet oxygen in cells. and / or fluorescence imaging of singlet oxygen.