A classic trace
iodine detection method is an
arsenic-
cerium catalytic spectrophotometric method, such as a sanitary
industry standard method WS / T 107-2016 for
urine iodine detection, and is widely applied to
iodine deficiency and iodine
nutrition monitoring in China. However, a highly toxic
arsenic trioxide (
arsenic trioxide)
reagent needs to be used in the method, and the application of the method is increasingly limited due to
environmental protection. A non-toxic trace iodine detection
reagent and a non-toxic trace iodine detection method are urgently needed in the field of health care. The invention discloses a
reagent and a method for detecting
iodide ions by adopting an iodine catalyzed
antimony-
cerium redox reaction principle.
Iodine ions catalyze Sb < 3 + > to reduce yellow Ce < 4+ > into colorless Ce < 3 + >, and the
reaction speed and the iodine content are in a
positive correlation quantitative relation. And after a certain reaction time, the
absorbance of the residual Ce< 4 + > in the solution is measured by using a spectrophotometric method, and the iodine content of the measured sample is calculated according to the quantitative relationship between the iodine content and the
absorbance. According to the method, toxic-free
antimony is adopted to replace highly-toxic arsenic in a classic method, the detection effect consistent with that of an arsenic-
cerium catalytic
spectrophotometry is achieved, the highly-toxic
arsenic trioxide reagent is prevented from being used, and the method is applied to detection of
urine iodine, blood iodine, water iodine, salt iodine, food iodine, soil iodine and the like and has important
public health significance.