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Reagent and method for detecting iodine through antimony-cerium reaction iodine catalysis method

A technology for detecting reagents and detection methods, which is applied in the field of reagents for detecting iodine ions, and can solve problems such as interference, poor stability, and low sensitivity

Pending Publication Date: 2020-08-14
刘列钧
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  • Application Information

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Problems solved by technology

However, the method is cumbersome and requires high technical requirements for the laboratory environment and personnel, and requires a dedicated laboratory and well-trained laboratory personnel.
The highly toxic chemical reagent diarsenic trioxide is used in the arsenic-cerium catalytic spectrophotometry. Its purchase, transportation, storage, use and waste liquid treatment should be in accordance with the "Management Measures for the Purchase and Road Transport License of Highly Toxic Chemicals" and "Safety Management of Hazardous Chemicals". Regulations and other regulations have brought many restrictions and inconveniences
[0005] Invention patent urine iodine rapid quantitative detection kit (patent number CN01133682.X) and reagents for quantitative detection of urinary iodine by biochemical analyzer and urine iodine detection method (patent number CN201110354278) are authorized patents of Liu Liejun, one of the inventors, These two methods have simplified and innovated the determination method, but they still use the principle of redox reaction of arsenic and cerium, and also need to use the highly toxic chemical reagent arsenic trioxide
[0008] Many other non-arsenic-cerium reaction determination systems of iodine have the problems of poor stability, low sensitivity, or interference by complex components of the sample, especially the obvious interference of the components of the solution to be tested after the pretreatment of urine, so that the scope of application is limited. Limitation, can not be used in the determination of urinary iodine

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  • Reagent and method for detecting iodine through antimony-cerium reaction iodine catalysis method
  • Reagent and method for detecting iodine through antimony-cerium reaction iodine catalysis method
  • Reagent and method for detecting iodine through antimony-cerium reaction iodine catalysis method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0174] Embodiment 1: Biochemical analyzer analysis method detects iodine content in urine.

[0175] Urine iodine determination is carried out by using the iodine ion detection method and reagent of the present invention.

[0176] (1) Reagent

[0177] (1.1) Digestion solution: ammonium persulfate solution {c[(NH 4 ) 2 S 2 o 8 ]=1.0mol / L};

[0178] (1.2) Reagent R1: antimony potassium tartrate-tartaric acid mixed solution, [c(C 8 h 4 o 12 Sb 2 ·3H 2 O)=0.005mol / L], [c(C 4 h 6 o 6 )=0.03mol / L];

[0179] (1.3) Reagent R2: ammonium cerium sulfate solution [c(Ce 4+ )=0.02mol / L];

[0180] (1.4) Iodine series standard solution: Potassium iodate solution with iodine content of 0, 50, 100, 200, 300, 400 μg / L respectively.

[0181] (2) Sample pretreatment: Take 0.25 mL each of the iodine series standard solution and urine sample and place them in glass test tubes, add 1.0 mL of digestion solution to each, mix well and place in a constant temperature heating device with a ...

Embodiment 2

[0202] Embodiment 2: Biochemical analyzer analysis method detects iodine content in serum.

[0203] Serum iodine determination is carried out by using the iodide ion detection method and reagent of the present invention.

[0204] (1) Reagent

[0205] (1.1) Digestion solution: perchloric acid (HC1O 4 , 70%~72%), sodium chlorate solution [c (NaC1O 3 ) =2.0mol / L];

[0206] (1.2) Reagent R1: antimony potassium tartrate-tartaric acid mixed solution, [c(C 8 h 4 o 12 Sb 2 ·3H 2 O)=0.005mol / L], [c(C 4 h 6 o 6 )=0.03mol / L];

[0207] (1.3) Reagent R2: ammonium cerium sulfate solution [c(Ce 4+ )=0.02mol / L];

[0208] (1.4) Iodine series standard solution: iodic acid solution, the iodine content is 0, 50, 100, 150, 200, 300, μg / L respectively.

[0209] (2) Sample pretreatment: Take 0.10 mL of iodine series standard solution and serum sample and place them in glass test tubes respectively, add 0.5 mL of perchloric acid and 0.6 mL of sodium chlorate solution, mix well and place...

Embodiment 3

[0227] Embodiment 3: Biochemical analyzer analysis method detects iodine content in drinking water.

[0228] The iodide ion detection method and reagent of the present invention are used for determination.

[0229] (1) Reagent

[0230] (1.1) Reagent R1: mixed solution of antimony potassium tartrate-tartaric acid, [c(C 8 h 4 o 12 Sb 2 ·3H 2 O)=0.005mol / L], [c(C 4 h 6 o 6 )=0.03mol / L];

[0231] (1.2) Reagent R2: ammonium cerium sulfate solution [c(Ce 4+ )=0.01mol / L];

[0232] (1.3) Iodine series standard solution: Potassium iodate solution with iodine content of 0, 20, 40, 60, 80, 100 μg / L respectively.

[0233] (2) The parameters of the biochemical analyzer (OLYMPUS-AU400) are set as follows:

[0234] (2.1) The measurement wavelength is 410nm;

[0235] (2.2) Draw the liquid to be tested and the amount of reagents: 120 μL for reagent R1, 40 μL for reagent R2, 30 μL for each iodine series standard solution and water sample;

[0236] (2.3) Reaction temperature: tempe...

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Abstract

A classic trace iodine detection method is an arsenic-cerium catalytic spectrophotometric method, such as a sanitary industry standard method WS / T 107-2016 for urine iodine detection, and is widely applied to iodine deficiency and iodine nutrition monitoring in China. However, a highly toxic arsenic trioxide (arsenic trioxide) reagent needs to be used in the method, and the application of the method is increasingly limited due to environmental protection. A non-toxic trace iodine detection reagent and a non-toxic trace iodine detection method are urgently needed in the field of health care. The invention discloses a reagent and a method for detecting iodide ions by adopting an iodine catalyzed antimony-cerium redox reaction principle. Iodine ions catalyze Sb < 3 + > to reduce yellow Ce < 4+ > into colorless Ce < 3 + >, and the reaction speed and the iodine content are in a positive correlation quantitative relation. And after a certain reaction time, the absorbance of the residual Ce< 4 + > in the solution is measured by using a spectrophotometric method, and the iodine content of the measured sample is calculated according to the quantitative relationship between the iodine content and the absorbance. According to the method, toxic-free antimony is adopted to replace highly-toxic arsenic in a classic method, the detection effect consistent with that of an arsenic-cerium catalytic spectrophotometry is achieved, the highly-toxic arsenic trioxide reagent is prevented from being used, and the method is applied to detection of urine iodine, blood iodine, water iodine, salt iodine, food iodine, soil iodine and the like and has important public health significance.

Description

technical field [0001] The invention belongs to the technical field of iodine detection in environment and biological samples, and relates to reagents and detection methods for detecting iodine content in urine iodine, blood iodine, water iodine, salt iodine and food in sanitation and medical examination, and specifically relates to a method using iodine catalysis Principle of antimony-cerium redox reaction, reagent and method for detecting iodide ion. Background technique [0002] The detection of iodine has important scientific significance in the fields of environment, sanitation and medicine. At present, the detection of iodine in urine, blood iodine, water iodine, salt iodine and food has become a routine detection item. About 90% of the iodine ingested by the human body is excreted from the urine after utilization. The urinary iodine level is an important indicator for evaluating the iodine nutritional status of the population, and urine iodine detection is an importa...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28
CPCG01N21/31G01N1/28
Inventor 刘列钧张聪何琴
Owner 刘列钧
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