The present invention discloses a urine iodine determination kit. According to the kit, based on the adsorbent, the dilution liquid, the coloring agent and the standard substance of the existing kit, a hydrogen peroxide solution, peroxyacetic acid solution, sodium hypochlorite solution or potassium permanganate solution with a mass percentage concentration of 5-12% is selected as an oxidizing agent and a special terminating liquid is arranged, and the terminating liquid comprises a sulfuric acid solution, hydrochloric acid solution, citric acid solution, sodium fluoride solution, sodium azide solution or potassium iodate solution with a mass percentage concentration of 1-20% or a combination comprising any solutions selected from the sulfuric acid solution, the hydrochloric acid solution, the citric acid solution, the sodium fluoride solution, the sodium azide solution and the potassium iodate solution according to any mass percentage ratio. According to the present invention, the single adsorbent and the single oxidizing agent are used so as to significantly reduce the cost, and the terminating liquid is used to rapidly terminate the reaction, such that it is ensured that the color is not significantly changed within a certain time, and the stable detection result coloring and the accurate detection result interpretation are achieved.