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Urine iodine test reagent kit

A kit and urine technology, applied in the field of in vitro diagnostic kits, can solve the problems of damage to human health, poor safety, inconvenience in colorimetric interpretation results with naked eyes, etc., and achieve the effects of improving accuracy and easy operation.

Active Publication Date: 2006-08-02
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is less secure
The reagent contains arsenic trioxide, which is likely to cause environmental pollution and harm to the human body; the toxic vapor produced during the digestion process is also likely to cause damage to human health
In addition, the analysis time of this method is long, and it takes more than 2 hours for digestion (ashing) and color development; the color development is light yellow, and special instruments are required, which is inconvenient for naked eye colorimetric interpretation of the results; special digestion and semi-automatic biochemical analyzers are required. Set up a special laboratory and fixed special personnel to operate, which is inconvenient to promote and apply at the grassroots level with serious iodine deficiency disorders

Method used

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  • Urine iodine test reagent kit

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Experimental program
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Effect test

Embodiment 1

[0037] 1. Choose figure 1 Shown urine purification device 10, this urine purification device is a kind of titration container, and selective specific adsorbent is housed in its titration container 3; The selective specific adsorbent of the present embodiment is the purified active carbon of 0.5g and 170ug of MgCl 2 The adsorbent composed; wherein the purified activated carbon is soaked in 10mmol / L citric acid buffer solution with pH=4.5; the selective specific adsorbent is activated by baking at 150°C for 36 hours; the activated selective specific adsorbent can completely adsorb Remove interfering substances in urine such as bilirubin, vitamin C, creatinine, etc. without adsorbing iodine;

[0038] 2. Prepare diluent: 10mmol / L citric acid buffer solution with pH=4.5 and MgCl with a mass percentage concentration of 1.85% 2 to mix;

[0039] 3. Chromogenic solution: a 9% by mass concentration of tetramethylbenzidine (TMB) aqueous solution is mixed with 9% by mass concentration ...

Embodiment 2

[0050] choose figure 1 Shown urine purification device 10, this urine purification device is a kind of titration container, and selective specific adsorbent is housed in its titration container 3; The selective specific adsorbent of the present embodiment is the purified activated carbon of 1g and 500ug of MgCl 2 The adsorbent composed; wherein the purified activated carbon is soaked in 10mmol / L citric acid buffer solution with pH=3.8; the selective specific adsorbent is activated by baking at 70°C for 72 hours; the activated selective specific adsorbent can completely adsorb Remove interfering substances in urine such as bilirubin, vitamin C, creatinine, etc. without adsorbing iodine;

[0051] Prepare diluent: 10mmol / L citric acid buffer solution with pH=3.8 and 5% MgCl in mass percent concentration 2 to mix;

[0052] Chromogenic solution: a 2% by mass concentration of tetramethylbenzidine (TMB) aqueous solution is mixed with 5% by mass concentration of polyethylene glycol...

Embodiment 3

[0057] choose figure 1 Shown urine purification device 10, this urine purification device is a kind of titration container, and selective specific adsorbent is housed in its titration container 3; The selective specific adsorbent of the present embodiment is the purified active carbon of 0.2g and 100ug of MgCl 2 The adsorbent composed; wherein the purified activated carbon is soaked in 10mmol / L citric acid buffer solution with pH=6.5; the selective specific adsorbent is activated by baking at 250°C for 4 hours; the activated selective specific adsorbent can completely adsorb Remove interfering substances in urine such as bilirubin, vitamin C, creatinine, etc. without adsorbing iodine;

[0058] Prepare diluent: 10mmol / L citric acid buffer solution with pH=6.5 and 0.1% MgCl in mass percent concentration 2 to mix;

[0059] Chromogenic solution: a 20% by mass concentration of tetramethylbenzidine (TMB) aqueous solution is mixed with 20% by mass concentration of polyethylene gly...

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Abstract

The present invention relates to a urine iodine determination kit. It includes urine purification device, diluent liquor, color-developing liquor, composite oxidant and standard colorimetric plate. Said kit can quickly remove interference substance from the urine, and can be used for accurately determining iodine concentration in the urine. The above-mentioned urine purification device is a titration type container in which a selective specific adsorbent for specifically adsorbing interference substance in the urine is held. Said selective specific adsorbent is made up by using purification active carbon and iodine adsorption agonist NaCl, MgCl2 or NaBr through baking activation process, the above-mentioned color-developing liquor is a mixed reagent formed from tetramethylbenzidine aqueous solution and polyethylene glycol. Besides, said invention also provides the composition of the composite oxidant.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic kits, in particular to a urine iodine determination kit for evaluating the iodine nutritional status of a human body by measuring the iodine concentration in urine. Background technique [0002] Iodine deficiency disorder is the most widely distributed in the world and the most harmful micronutrient deficiency disease to human mental health. The classic urine iodine determination method is the Sandell-Kolthoff reaction, which is recommended by WHO and IDDICC. The basic principle is that iodine catalyzes the reduction of tetravalent cerium to trivalent cerium, which reduces the concentration of cerium-arsenic complex, resulting in a decrease in the absorbance at 405nm. In order to remove interfering substances in the urine, the urine must be digested with perchloric acid at 110°C, or the specimens should be ashed in a high-temperature furnace before determination. Because the digestive agent pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/25G01N1/34G01N1/38
Inventor 王加义
Owner 北京中生金域诊断技术股份有限公司
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