Detection method of iodine ions in urine based on alcohol solvent-induced silver nanocluster fluorescence enhancement

A technology of silver nanoclusters and fluorescence enhancement, which is applied in the field of biomedical analysis and detection, can solve the problems of time-consuming sample pretreatment process, high environmental requirements, complex operation, etc., and achieve the effect of visual quick measurement, high sensitivity and enhanced fluorescence intensity

Active Publication Date: 2017-08-22
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the traditional detection methods mainly include: atomic absorption spectrometry, ion selective electrode method, inductively coupled plasma-mass spectrometry, gas chromatography, ion chromatography and capillary electrophoresis, etc., but most of these methods are more complicated to operate

Method used

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  • Detection method of iodine ions in urine based on alcohol solvent-induced silver nanocluster fluorescence enhancement
  • Detection method of iodine ions in urine based on alcohol solvent-induced silver nanocluster fluorescence enhancement
  • Detection method of iodine ions in urine based on alcohol solvent-induced silver nanocluster fluorescence enhancement

Examples

Experimental program
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Effect test

Embodiment 1

[0037] To prepare alcohol-induced fluorescence-enhanced silver nanoclusters (AgNCs) probes, the steps are as follows:

[0038] 1) Preparation of silver nanoclusters (AgNCs) materials:

[0039] a) At room temperature, add 125 μL of 20 mM silver nitrate (AgNO 3 ) Mix the aqueous solution with 150 μL of 50 mM glutathione (GSH), then add 5.0 mL of ultrapure water, stir and mix evenly, adjust the pH to 9-10 with 1.0 M NaOH aqueous solution to obtain solution A;

[0040] b) Combine 4.2 mg α-lipoic acid (LA) with 1.9 mg sodium borohydride (NaBH 4 ) Mix with 1 ml of ultrapure water, stir until LA is completely dissolved to obtain solution B, add 700 μl of solution B dropwise to solution A, stir for 20 min, let stand at room temperature for 1.5 h, then centrifuge After separation and purification, the silver nanocluster (AgNCs) material was prepared, and stored at 4°C and protected from light for later use;

[0041] 2) Preparation of silver nanoclusters (AgNCs) probes with alcohol-induced fluo...

Embodiment 2

[0044] Based on the alcohol solvent-induced silver nanocluster fluorescence enhancement method for detecting iodide ions in urine, the alcohol-induced fluorescence enhancement silver nanocluster probe prepared in Example 1 is used to determine iodide ions in urine (children’s morning urine), and the steps include:

[0045] The alcohol-induced fluorescence-enhanced silver nanocluster probes were sequentially added to an equal volume (100 μl) with a concentration gradient (0.050, 0.10, 0.25, 0.50, 0.75, 1.0, 2.5, 5.0, 7.5, and 10 μM). I - In the urine, the probe was added at a concentration of 1.05 mM, using a 1.0 M HCl or NaOH aqueous solution, adjusting the pH to 6-8, reacting for 5 min, and observing the color of the solution under a UV projector with a wavelength of 365 nm Use a fluorescence spectrometer to measure the fluorescence spectrum and intensity at the maximum excitation wavelength of 425nm, and draw a calibration curve; according to the above method, measure the fluore...

Embodiment 3

[0047] Urine iodine ion detection method based on alcohol solvent-induced fluorescence enhancement of silver nanoclusters, the alcohol-induced fluorescence-enhanced silver nanocluster probe prepared in Example 1 is used to determine iodine ions in urine to be tested (morning urine of pregnant women), and the steps are the same Example 2, the results are shown in Table 1.

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Abstract

The invention relates to a detection method of iodine ions in urine based on alcohol solvent-induced silver nanocluster fluorescence enhancement. The detection method comprises the following steps: adopting a biomineralization way to prepare a material with silver nanoclusters firstly, changing selective recognition and response performance of the silver nanoclusters to anions by utilizing alcohol solvent induction to form an alcohol-induced fluorescence enhanced silver nanocluster probe, and establishing a simple, convenient, rapid, specific, sensitive and visual iodine ion fluorescence detection method to realize specific fast detection of the iodine ions in a complex human excrement sample such as the urine. A detection result is comparable to that of a typical instrument detection method, the urine iodine detection process does not involve complex instrument operation, and the detection method has the advantages of simpleness, convenience, rapidness, specificity, high sensitivity, visualization and the like and is expected to be widely applied to the biological and medical monitoring and detection field.

Description

Technical field [0001] The invention belongs to the technical field of biomedical analysis and detection, and relates to a method for detecting iodine ions in urine based on alcohol solvent-induced fluorescence enhancement of silver nano clusters. Background technique [0002] Solvent effects can be divided into general solvent effects and special solvent effects. Generally, the solvent effect is caused by the physical properties of the solvent. It is the result of all solvents acting together on the fluorescent substance. It is related to physical quantities such as the solvent’s refractive index and dielectric constant. With the change of the above properties, the relaxation effect will cause fluorescence emission. The peaks appear red-shift or blue-shift; the special solvent effect is produced by the chemical interaction between the substance and the solvent molecule, and generally depends on the structural formula of the solvent and the fluorescent substance. For example, Hu...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 王桦冯路平刘敏刘欢张生许吉宏陈丽君段志强
Owner QUFU NORMAL UNIV
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