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A method for the detection of iodide ions in urine based on the fluorescence enhancement of silver nanoclusters induced by alcohol solvent

A silver nanocluster, fluorescence enhancement technology, applied in the field of biomedical analysis and detection, can solve the problems of time-consuming sample pretreatment process, high environmental requirements, time-consuming measurement, etc., and achieve the effects of visual rapid measurement, high sensitivity, and easy operation.

Active Publication Date: 2021-03-23
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the traditional detection methods mainly include: atomic absorption spectrometry, ion selective electrode method, inductively coupled plasma-mass spectrometry, gas chromatography, ion chromatography and capillary electrophoresis, etc., but most of these methods are more complicated to operate, High cost, time-consuming or pretreatment process that requires complex samples, etc.
For example, although gas chromatography is easy to operate and is suitable for the determination of a large number of samples, the instrument is large, expensive, time-consuming and requires high environmental requirements.

Method used

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  • A method for the detection of iodide ions in urine based on the fluorescence enhancement of silver nanoclusters induced by alcohol solvent
  • A method for the detection of iodide ions in urine based on the fluorescence enhancement of silver nanoclusters induced by alcohol solvent
  • A method for the detection of iodide ions in urine based on the fluorescence enhancement of silver nanoclusters induced by alcohol solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of silver nanoclusters (AgNCs) probes with alcohol-induced fluorescence enhancement, the steps are as follows:

[0038] 1) Preparation of silver nanoclusters (AgNCs) materials:

[0039] a) At room temperature, 125 μL of 20 mM silver nitrate (AgNO 3 ) aqueous solution and 150 μL of 50 mM glutathione (GSH) were mixed, then 5.0 mL of ultrapure water was added, stirred and mixed evenly, and the pH was adjusted to 9-10 with 1.0 M NaOH aqueous solution to obtain solution A;

[0040] b) Mix 4.2 mg α-lipoic acid (LA) with 1.9 mg sodium borohydride (NaBH 4 ) mixed with 1 ml of ultrapure water, and stirred until LA was completely dissolved to obtain solution B. Take 700 μl of solution B and add it to solution A drop by drop. After stirring for 20 min, let it stand at room temperature for 1.5 h, and then centrifuge Separation and purification to obtain silver nanoclusters (AgNCs) materials, stored at 4°C in the dark, for later use;

[0041] 2) Preparation of silver ...

Embodiment 2

[0044] The method for detecting iodide ions in urine based on alcohol solvent-induced fluorescence enhancement of silver nanoclusters, using the silver nanocluster probe with alcohol-induced fluorescence enhancement prepared in Example 1 to measure iodide ions in urine (morning urine of children), the steps include:

[0045] The alcohol-induced fluorescence-enhanced silver nanocluster probes were sequentially added to equal volumes (100 μl) of the solution containing concentration gradients (0.050, 0.10, 0.25, 0.50, 0.75, 1.0, 2.5, 5.0, 7.5 and 10 μM). I - In the urine, the concentration of the probe was 1.05 mM, and the concentration of 1.0 M HCl aqueous solution or NaOH aqueous solution was used to adjust the pH to 6-8, react for 5 minutes, and observe the color change of the solution under an ultraviolet projector with a wavelength of 365 nm. , and using a fluorescence spectrometer, measure its fluorescence spectrum and intensity at the maximum excitation wavelength of 425 ...

Embodiment 3

[0047] The method for detecting iodide ions in urine based on alcohol solvent-induced fluorescence enhancement of silver nanoclusters uses the alcohol-induced fluorescence-enhanced silver nanocluster probe prepared in Example 1 to measure iodide ions in the urine to be tested (morning urine of pregnant women), and the steps are the same as the implementation Example 2, the results are shown in Table 1.

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Abstract

The invention relates to a detection method of iodine ions in urine based on alcohol solvent-induced silver nanocluster fluorescence enhancement. The detection method comprises the following steps: adopting a biomineralization way to prepare a material with silver nanoclusters firstly, changing selective recognition and response performance of the silver nanoclusters to anions by utilizing alcohol solvent induction to form an alcohol-induced fluorescence enhanced silver nanocluster probe, and establishing a simple, convenient, rapid, specific, sensitive and visual iodine ion fluorescence detection method to realize specific fast detection of the iodine ions in a complex human excrement sample such as the urine. A detection result is comparable to that of a typical instrument detection method, the urine iodine detection process does not involve complex instrument operation, and the detection method has the advantages of simpleness, convenience, rapidness, specificity, high sensitivity, visualization and the like and is expected to be widely applied to the biological and medical monitoring and detection field.

Description

technical field [0001] The invention belongs to the technical field of biomedical analysis and detection, and relates to a method for detecting iodide ions in urine based on the fluorescence enhancement of silver nanocluster induced by alcohol solvent. Background technique [0002] Solvent effects can be divided into general solvent effects and special solvent effects. Generally, the solvent effect is caused by the physical properties of the solvent. It is the result of the joint action of all solvents on fluorescent substances. It is related to physical quantities such as solvent refractive index and dielectric constant. With the change of the above properties, the influence of relaxation effect, the fluorescence emission The peak appears to be red-shifted or blue-shifted; and the special solvent effect is produced by the chemical interaction between the substance and the solvent molecule, and generally depends on the structural formula of the solvent and the fluorescent su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 王桦冯路平刘敏刘欢张生许吉宏陈丽君段志强
Owner QUFU NORMAL UNIV
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