47results about How to "Strong response signal" patented technology

The invention provides construction of a surface plasma resonance sensor based on the amplification effect of a magnetic molecularly imprinted polymer and the application of the surface plasma resonance sensor in pesticide detection, and belongs to the technical field of surface plasma resonance sensing. Chlorpyrifos is regarded as a template molecule firstly; the magnetic molecularly imprinted polymer which has recognition action on the chlorpyrifos is compounded by adopting the self-polymerization performance of dopamine; after elution is performed on the chlorpyrifos, the magnetic molecularly imprinted polymer containing imprinted cavities has good superparamagnetism and high selectivity; the specific interaction between acetylcholinesterase fixed on the surface of a gold sheet and the chlorpyrifos is adopted to construct a pesticide residue recognition, detection and sensing interface on the basis of the amplification effect of the magnetic molecularly imprinted polymer. Compared with the conventional pesticide residue detection technology, the surface plasma resonance sensor on the basis of the amplification effect of the magnetic molecularly imprinted polymer has high detection sensitivity and good selectivity to chlorpyrifos detection, and has an excellent application prospect.

The invention discloses a preparation method of a Cu2Se-Pd hybrid material and a prepared material. A copper source is tetrachloro cupric (II) acid tetraethylammonium, and a selenium source is a mixed liquid formed by dissolving SeO2 into terpilenol. The preparation method comprises the following steps: performing reaction in DMPU (dimethyl propyl urea) to obtain hexagonal phase CuSe or cubic phase Cu2-XSe by virtue of adopting a solid-liquid phase synthetic method; further extracting a product by virtue of TOP to obtain mono-clinic phase Cu2Se; compounding the mono-clinic phase Cu2Se with noble metal Pd to prepare the Cu2Se-Pd hybrid material. The method disclosed by the invention can be used for synthesizing the nano-level Cu2Se-Pd hybrid material under normal pressure and relatively low temperature, and is simple in process and short in reaction time; the prepared hybrid material can be used as an SERS (surface enhanced raman spectroscopy) substrate so as to be applied to SERS detection 4-Mpy, and is strong in response signal and high in selectivity; the limit of detection can reach 10<-9>molL<-1>. Compared with independent semiconductor Cu2Se and metal Pd, the Cu2Se-Pd hybrid material shows more excellent SERS performances.

The invention discloses an instrument-free enzyme-linked immunosorbent assay method. According to the method, an antigen-antibody specific immune reaction is carried out in a 96-pore plate so as to capture an analyte antigen or antibody in a sample; nanoparticles are introduced to catalyze a reaction for generating nanogold between chloroauric acid and a reducing agent; a watch or a mobile phone is used for recording the time required for mixed solution to become red, so as to complete instrument-free enzyme-linked immunosorbent assay, wherein the time is negatively correlated to the concentration of a target antigen or antibody in the sample. According to the method, widespread watches or mobile phones are used for carrying out signal reading, so that the cost of quantitative analysis isreduced. Through collaborative use of lipidosome for wrapping plenty of nanoparticles and efficient catalytic activity of the nanoparticles for the reaction of reducing the chloroauric acid by the reducing agent to generate the nanogold, the response signals for the specific binding reaction of single antigen-antibody are amplified, so that the detection sensitivity is improved.

The invention discloses application of a self-assembled film in a surface auxiliary laser adsorption or ionization flying time mass spectrum. The application is characterized in that the invention relates to the application of the self-assembled film made of an aromatic thiol compound in the surface auxiliary laser adsorption or ionization flying time mass spectrum. The application comprises the following steps of: firstly, preparing the aromatic thiol compound self-assembled film for surface auxiliary laser desorption or ionization of small molecules, namely preprocessing a silicon slice, sequentially sputtering Ti and Au on the preprocessed silicon slice to obtain a golden substrate, and putting the golden substrate into an ethanol solution with the aromatic thiol compound for generating self-assembling reaction to obtain the aromatic thiol compound self-assembled film; and then drying small-molecular sample points on the surface of the obtained self-assembled film, and placing the self-assembled film into an MALDI-TOF-MS mass spectrum for analysis. The application has the advantages that the substrate is stable; an Au layer is difficult to fall off; the small peptide and the traditional Chinese medicine active components can be conveniently and quickly analyzed; an analysis effect is good; and a response signal is high.

The invention relates to a transient electromagnetic detection method and system, belongs to the technical field of geophysical exploration, and specifically relates to a side-excited loop source ground well transient electromagnetic detection method and system. By exciting an anomalous body from the side through an ungrounded loop, a good coupling effect can be produced on a steep or upright low-resistance slab body. By using a receiving device to receive secondary field signals in a roadway or borehole, strong response signals can be received. By using a proposed data processing method to invert the received signals, an accurate interpretation result can be obtained.

The invention discloses a metal pipeline detecting system based on a time-domain electromagnetic method. The metal pipeline detecting system has a proximal connecting point, a distal connecting point,a transmitter, a transmitting connecting line, a measuring machine and a receiving device. The system has a rectangular detecting area of which the short side is perpendicular to the approximate direction of a target pipeline, and of which the long side is parallel to the approximate direction of the target pipeline. The proximal connecting point is located at an exposed point of the target pipeline and corresponds to a vertex of the rectangular detecting area. The distal connecting point is located at a vertex of the rectangular detecting area adjacent to the proximal connecting point. The transmitting connecting line is disposed on the side of the detecting area. The receiving device is connected to the measuring machine through a mounting frame. The measuring machine and the transmitter are timed and set parameters before detection. The system has the advantages that the to-be-detected section of the target pipeline is directly put in the transmission loop in order to guarantee that an excitation field source is applied to the target pipeline to the utmost extent, a response signal is stronger and the side electromagnetic interference can be reduced, and the to-be-detected pipeline can be precisely positioned, orientated and continuously tracked.

The invention belongs to the fields of novel nano materials, immuno-assay, and biosensor, provides a preparation method and applications of a current mode immuno-sensor for detecting breast cancer, and specifically relates to a construction method of an electrochemical immuno-sensor, which is made of a graphene nano composite material based on palladium loaded nano rod copper oxide functionalization, and an application of using the electrochemical immuno-sensor to detect a specific antigen of breast cancer.

The invention relates to the field of SPR sensor chips, and particularly relates to a ractopamine molecularly imprinted SPR sensor chip as well as a preparation method thereof. A ractopamine molecularly imprinted polymer, graphene nanogold, and 1-octyl-3- methylimidazole hexafluorophosphate are taken as chip substrate modifiers for the ractopamine molecularly imprinted SPR sensor chip. The ractopamine molecularly imprinted SPR sensor chip provided by the invention has high-specificity adsorption capability to ractopamine, and has obvious response signal variation to low-concentration ractopamine in samples. The ractopamine molecularly imprinted SPR chip prepared by the invention is simple in steps, is short in analysis time, and has relatively high accuracy and flexibility when being used for analyzing the low-concentration ractopamine residue.

The invention relates to a sensor chip and a manufacturing method thereof, and belongs to the technical field of designing and manufacturing of surface plasma resonance sensor chips. The chip adopts a composite structure formed by a glass substrate, a metallic film, a self-assembly film, and a metallic oxide and polymer composite thin film, in sequential arrangement from bottom to top; and nanometer metallic oxide is successfully fixed to the surface of the sensor chip by a polymer embedding method, so that the detection range of objective gas is effectively enlarged, and the sensitivity of the objective gas is effectively improved. The method disclosed by the invention is simple, the manufactured sensor chip has high sensitivity on sulphur dioxide, detection requirements under room temperature condition are met, and the sensor chip has favorable response signals within 5 ppm-40 ppm.

The invention discloses a preparation method of an electrochemical sensor for detecting molecular imprinting of organophosphorus pesticide residue. The method comprises the following steps of: firstly preparing a solution from the raw molecules of organophosphorus pesticide serving as imprinting molecules and a functional monomer and an electrolyte; depositing the molecular imprinting polymer on the surface of a working electrode by an electrochemical polymerization method so as to finish the preparation of a molecular imprinting sensitive film; and removing the imprinting molecules of the organophosphorus pesticide from the molecular imprinting sensitive film by a proper elution method and a proper eluent so as to finish the preparation of the electrochemical sensor. The method disclosed by the invention has the advantages of fast response, high sensitivity and good selectivity; and meanwhile, the detection method is simple and quick, has strong adaptability after being popularized and particularly strong universality in pesticide detection, and has a wide market prospect.

The invention discloses a mercury ion electric sensing analysis and detection method based on excision enzyme III assisted target circulation and subject and object recognition. The mercury ion electric sensing analysis and detection method comprises the following steps that a glassy carbon electrode is polished, ground and cleaned; nanogold is subjected to electrolytic deposition on the surface of the glassy carbon electrode, and thus a nanogold modified electrode is prepared; aminated beta-cyclodextrin is modified on nanogold particles to prepare a beta-cyclodextrin modified electrode, Hg<2+> is added into a mixed solution of DNA A1 and DNA A2 to form a double-chain solution; ferrocene marked DNA A3 is added into the double-chain solution to be incubated, and thus a T-shaped DNA structure is formed; exonuclease III is added into the T-shaped DNA structure to form a mixed solution; the mixed solution is dropwise added to the surface of the beta-cyclodextrin modified electrode; the modified electrode is immersed into a testing base solution to detect the electrochemical properties of the modified electrode, and quantitative measurement of Hg<2+> is achieved according an electrochemical signal; and the linearity and the selectivity of an electrochemical biological sensor are tested.

The invention relates to a sensor chip capable of detecting sulfur dioxide under normal temperature conditions and a preparation method thereof, belonging to the technical field of surface plasmon resonance sensor chip design and preparation. The structure of the sensor chip includes: a glass substrate, a metal film, a self-assembled film, and a guanidine-containing polymer film; the invention synthesizes a new compound monomer by using a guanidine compound and an acrylic compound. After self-assembly modification on the surface of the gold-plated glass substrate, a guanidinium-containing polymer film was prepared on the surface plasmon resonance sensor substrate by thermal polymerization. The sensor can detect sulfur dioxide gas under normal temperature conditions, and has fast detection response speed and high sensitivity; it can be applied to SPR detection, electrochemical detection and QCM detection.

The invention discloses a terahertz radio frequency signal detector based on a metasurface optical antenna and a preparation method. The detector comprises a substrate, a doping layer, a silicon dioxide layer, a metasurface optical antenna layer, an ohmic electrode, a Schottky electrode and a common electrode. The width of the metasurface optical antenna layer is 2-100 mm, and the metasurface optical antenna layer comprises a first metal layer used for detecting radio frequency S wave band signals or C wave band signals or X wave band signals and a second metal layer used for detecting terahertz signals. Since the first metal layer and the second metal layer respectively have extremely strong local surface plasmon induction capability on radio frequency and terahertz signals, once local surface plasmon oscillation is generated with a corresponding signal, the response speed belongs to ultra-high-speed response, and an extremely strong response signal can be generated in an extremely short time, so that the detector can better distinguish an electromagnetic signal of a terahertz radio frequency band. In addition, due to the fact that the super-surface optical antenna is manufacturedthrough the nanometer technology, the terahertz radio frequency signal detector is very small in size and very light in weight.

The invention belongs to the technical fields of novel nanomaterials, immunoassay and biosensing detection and provides a preparation method and application of an mHPBs-Hemin/rGO marked electrochemical immunosensor. According to the preparation method, a mesoporous hollow Prussian-blue nanocube spaced hemin functional graphene porous composite is used as a current signal amplifying mechanism and introduced into construction of the electrochemical immunosensor for the first time, a current response signal is amplified efficiently, and quantitative and sensitive detection of aflatoxin is realized. The electrochemical immunosensor is easy and convenient to operate, low in cost, high in sensitivity and wide in detection range and has important scientific significance and application value forquantitative and sensitive detection of aflatoxin.

The invention discloses a visual optic sensing method for detecting a target-mediating agglutination reaction of nano-gold probes on the basis of Tyndall effect. In the method, by means of an analyticsubstance in a sample solution, a reaction solution, which contains a target capable of mediating the agglutination reaction of naked nano-gold probes or biofunctionalized composite nano-gold probes,can be obtained; then the reaction solution is mixed and reacted with a nano-gold probe solution in order to observe an optical signal of the Tyndall effect of the mixed solution under laser beam formed by a laser pen or a laser device, wherein the signal intensity is in positive correlation with the concentration of the analytic substance. Through a cheap portable device for reading the signal (i.e. the optical signal of the Tyndall effect), low-cost portable quantitative detection of the analytic substance is achieved. The method has simple operations and low cost, is free of special analytic instruments and equipment and is suitable for on-site analysis and real-time detection, and can be directly promoted in the fields such as medical diagnosis, environment monitoring, food safety andthe like.

The invention discloses a colibacillus O157:H7DNA label-free detection device and method based on LAPS and ZnO nanorod array. The device comprises a Faraday shield box, a loading table, a semiconductor laser, an LAPS sensor, a manostat, a lock-in amplifier, an acquisition card, and a computer. The method comprises of performing bacterial culture, extracting colibacillus O157:H7DNA, manufacturing aLAPS sensor and modifying a ZnO nanorod; modifying probe chain DNA on a surface of the LAPS sensor, and scanning an I-V curve; fitting an optimal calibration curve detecting concentration of O157:H7DNA through traversing and a least square method; and analyzing an I-V curve of a to-be-tested sample solution and voltage offset V and substituting into the calibration curve, and calculating concentration of the to-be-tested sample. The method realizes label-free detection of O157:H7 DNA, and has advantages of short time, low cost, convenience in operation, 3D site for O157:H7 DNA hybrid, and high sensitivity.