Construction and application of SPR sensor based on amplification effect of magnetic molecularly imprinted polymer

A magnetic molecular imprinting and polymer technology, applied in the measurement of scattering characteristics, etc., can solve the problems of reducing the utilization rate of imprinting sites, large particle diffusion resistance, difficult elution of template imprinted molecules, etc., and achieve fast binding/dissociation rate, The effect of improving binding ability and good application prospect

Inactive Publication Date: 2013-12-04
NANCHANG UNIV
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  • Abstract
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Problems solved by technology

At present, the simple and easy bulk polymerization method is the most commonly used method to prepare MIPs. However, the prepared highly cross-linked polymer network, the internal template imprinted molecules are not easy to elute, the internal diffusion resistance of the particle is large, and the imprinted particles embedded in the particle The site cannot function, reducing the utilization of the imprinted site

Method used

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  • Construction and application of SPR sensor based on amplification effect of magnetic molecularly imprinted polymer
  • Construction and application of SPR sensor based on amplification effect of magnetic molecularly imprinted polymer
  • Construction and application of SPR sensor based on amplification effect of magnetic molecularly imprinted polymer

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Experimental program
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Effect test

Embodiment 1

[0026] (1) Fe 3 o 4 Preparation of NPs: 2.35g FeSO 4 ·7H 2 O and 4.1 g FeCl 3 ·6H 2 O was dissolved in 90mL of water, and 25mL of ammonia water with a concentration of 25% by mass was quickly added at room temperature, the solution turned from orange to black, and then 1mL of oleic acid was added dropwise, and then Under the reaction for 1h, Fe with an average particle size of 6-7nm was obtained 3 o 4 NPs. The above experimental process needs to be carried out under the protection of nitrogen. Finally, the Fe in the solution is 3 o 4 NPs were isolated and stored at 4°C for future use;

[0027] (2) Preparation of magnetic molecularly imprinted polymer: 50mg Fe 3 o 4 Dissolve NPs in 20mL, 10mM, pH8.0 Tris buffer solution, add 5mL acetonitrile solution containing 1mg / mL CPF, mechanically stir for 2h at room temperature, then add 25mg DA, continue stirring for 4h at room temperature; the reaction product is separated by a magnet , the imprinted CPF molecules and unpo...

Embodiment 2

[0032] Construction of SPR sensing interface

[0033] (1) The construction process of the SPR sensing interface is as follows: figure 1 shown. First place the gold flakes in H with a volume ratio of 7:3 2 SO 4 :H 2 o 2 Soak in the mixed solution for 2 minutes, rinse with secondary water and blow dry with nitrogen, and install it into the SPR detection cell. Inject 50 μL of 1 mM 11-mercaptoundecanoic acid solution into the detection cell to react for 2 hours, rinse with secondary water, inject 50 μL of 0.2M 1-ethyl-3-(3-dimethylaminocarbodiimide) hydrochloride and 0.05M N-hydroxysuccinimide mixed solution was reacted for 10 minutes, and then injected with 50 μL 2U / mL AChE solution for 30 minutes; finally, 50 μL of 1.0M ethanolamine solution was injected for 10 minutes, and after washing with 10 mM Tris buffer solution of pH 8.5, the AChE functional Optimized SPR sensing interface;

[0034] (2) Combining CPF with MIP-Fe 3 o 4 PDA NPs were mixed at room temperature and i...

Embodiment 3

[0037] (1) Influence of polymerization time, pH of polymerization solution, and pH of recombination medium solution on sensor performance

[0038] Image 6 A is the effect of DA aggregation time on sensor performance. It can be seen from the figure that as the polymerization time prolongs, the SPR angle change (ΔAngle) gradually increases. When the polymerization time is 4h, ΔAngle reaches the maximum, and after more than 4h, ΔAngle decreases. If the polymerization time is too short, the number of imprinted sites is small, resulting in weak adsorption capacity for CPF; if the polymerization time is too long, the thickness of the PDA film will be large, and the elution efficiency of CPF will be low, which will lead to a decrease in the adsorption capacity of molecular imprinting. Therefore, the best polymerization time for choosing DA is 4h. Image 6 B is the effect of the pH of the polymerization solution on the performance of the sensor. Lower pH will inhibit the self-poly...

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Abstract

The invention provides construction of a surface plasma resonance sensor based on the amplification effect of a magnetic molecularly imprinted polymer and the application of the surface plasma resonance sensor in pesticide detection, and belongs to the technical field of surface plasma resonance sensing. Chlorpyrifos is regarded as a template molecule firstly; the magnetic molecularly imprinted polymer which has recognition action on the chlorpyrifos is compounded by adopting the self-polymerization performance of dopamine; after elution is performed on the chlorpyrifos, the magnetic molecularly imprinted polymer containing imprinted cavities has good superparamagnetism and high selectivity; the specific interaction between acetylcholinesterase fixed on the surface of a gold sheet and the chlorpyrifos is adopted to construct a pesticide residue recognition, detection and sensing interface on the basis of the amplification effect of the magnetic molecularly imprinted polymer. Compared with the conventional pesticide residue detection technology, the surface plasma resonance sensor on the basis of the amplification effect of the magnetic molecularly imprinted polymer has high detection sensitivity and good selectivity to chlorpyrifos detection, and has an excellent application prospect.

Description

technical field [0001] The invention relates to the construction of an SPR sensor based on the amplification effect of magnetic molecularly imprinted polymers and the application of the SPR sensor in pesticide detection, belonging to the technical field of surface plasmon resonance sensing. Background technique [0002] Molecular imprinting technology refers to the preparation technology of preparing polymers that completely match the template molecules in space and binding sites. Molecularly imprinted polymers (MIPs) have been widely used in various scientific fields due to their mechanical / chemical stability, low cost, and ease of preparation. At present, the simple and easy bulk polymerization method is the most commonly used method to prepare MIPs. However, the prepared highly cross-linked polymer network, the internal template imprinted molecules are not easy to elute, the internal diffusion resistance of the particle is large, and the imprinted particles embedded in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/55
Inventor 梁汝萍姚桂红邱建丁
Owner NANCHANG UNIV
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