103results about How to "Enhanced response signal" patented technology

A CARS system and method for probing a Raman signature of a sample are described. A short femtosecond pulse from one or more lasers is split into two pulses, a stimulus pulse and a probe pulse, whereby the probe pulse is time-delayed with respect to the stimulus pulse. Both pulses can be phase- and polarization-modulated. The stimulus pulse excites a vibronic level in the sample and the probe pulse probes molecular Raman transitions in the sample. A difference in the signals with and without excitation can be used to determine on which molecule a bond is most likely located. This will allow an accurate and sensitive determination of the presence of specific molecules in the sample. The system and method can be used to analyze biological samples and to discriminate between molecules having overlapping Raman signatures.

An object locating system employing tags and a handheld locator. A unique low power tag circuit enables highly compact form factor and extremely long operating life even from the smallest available batteries. The tag employs an efficient passive RF energy detector circuit rather than an active receiver, as well as a novel low-power temperature-compensated biasing circuit to provide uniform sensitivity over a broad temperature range. The locator device transmits a tag activation signal, receives tag RF responses, and reports presence and optionally proximity changes to the user. An alternate tag design directly conveys proximity changes. The locator may also incorporate tag functionality to aid in finding a misplaced locator.

A CARS system and method for probing a Raman signature of a sample are described. A short femtosecond pulse from one or more lasers is split into two pulses, a stimulus pulse and a probe pulse, whereby the probe pulse is time-delayed with respect to the stimulus pulse. Both pulses can be phase- and polarization-modulated. The stimulus pulse excites a vibronic level in the sample and the probe pulse probes molecular Raman transitions in the sample. A difference in the signals with and without excitation can be used to determine on which molecule a bond is most likely located. This will allow an accurate and sensitive determination of the presence of specific molecules in the sample. The system and method can be used to analyze biological samples and to discriminate between molecules having overlapping Raman signatures.

The invention relates to a method and a device for detecting a polymer film ion selective electrode. The method comprises the following steps of: putting a nucleic acid aptamer and polycation into a detection pond, and inserting a polycation selective electrode into the detection pond to generate a comparison signal; adding the nucleic acid aptamer into the detection tank, reacting with standard solution of a substance to be detected, adding the polycation, and inserting the polycation selective electrode into the detection pond to generate standard signals of the standard solution of the substance to be detected at different concentrations; obtaining a standard curve according to the standard signals and the comparison signal; adding liquid to be detected into the detection pond, adding the nucleic acid aptamer, reacting with the liquid to be detected, adding the polycation in the ratio to conduct the signals, and inserting the polycation selective electrode into the detection pond to generate a sample signal; and acquiring a signal to be detected according to the sample signal and the comparison signal, and acquiring the concentration of the liquid to be detected by the reference of the standard curve. The method is wide in application range, high in generality, large in electrode response signals and obvious in potential change, so the method is a marking-free and immobilization-free method.

The invention discloses a chip of a signal enhancer used for a RF identifying system; the chip of the signal enhancer includes a receiving circuit, a transmitting circuit and a power module; the receiving circuit includes a filter, a demodulation amplifying module and a digital quantification module. The transmitting circuit includes a logic control module and a demodulation module. The signal transmitted to the signal enhancer by a double interface SIM card is received by the input end of the filter; the output end of the filter is connected with the input end of the demodulation amplifying module and then is output to the input end of the digital quantification module and then is output to the input end of the logic control module. The logic control module outputs the signal to the input end of the demodulation module. The demodulation module generates a subcarrier which reflects and demodulates the signal and returns the signals to a reader. The signal enhancer effectively solves the problem of signal attenuation by utilizing each module inside the chip of the signal enhancer and realizes the non-contact close-distance RF identification on a mobile phone under the situations of not changing the movable communication terminals like the existing RFID reader and the mobile phones.

The invention discloses a preparation method for a nano-material used for detection of glucose. The method comprises the following steps: first, a metal salt water solution with a concentration of 100-200mM is prepared; second, glucose oxidase and peroxidase are mixed and a buffer solution is prepared; third, the solution obtained in the first step and the solution obtained in the second step are mixed at a volume rate of 1:150; fourth, the mixed solution obtained in the third step is mixed uniformly, and the sediment obtained after standing is the nano-material. Through the method, hybrid nanoflowers with high catalytic activity can be synthesized simply and efficiently, experiment operation steps are simplified, and the response signal of a glucose colorimetric detection system to glucose is raised greatly.

The invention discloses a method for manufacturing a nano platinum and multiwalled carbon nanotube modified glassy carbon electrode. The method comprises the following steps: step one, performing ultrasonic treatment on multiwalled carbon nanotubes in an acid solution; step two, dispersing the multi-walled carbon nanotubes processed in the step one in a glycol aqueous solution, and dispersing uniformly through ultrasonic waves to obtain a multi-walled carbon nanotube solution; step three, polishing a bare glassy carbon electrode on alumina powder, then carrying out ultrasonic treatment in absolute ethyl alcohol and water in sequence; step four, dispensing the multi-walled carbon nanotube solution obtained in the step two on the surface of the bare glassy carbon electrode processed in the step three and drying to obtain a multiwalled carbon nanotube modified glassy carbon electrode ; step five, putting the multiwalled carbon nanotube modified glassy carbon electrode processed in the step four in a chloroplatinic acid aqueous solution and carrying out constant potential deposition to obtain the nano platinum and multiwalled carbon nanotube modified glassy carbon electrode. According to the method, the response signal of estradiol can be improved, and the detection reliability for estradiol detection is improved.

The invention relates to a multifunctional flexible sensing material and a preparation method and application thereof. The multifunctional flexible sensing material comprises a magneto-rheological layer and an electric conduction layer. The magneto-rheological layer has a magneto-rheological effect and can be subjected to stress strain under the effect of a magnetic field; the electric conductionlayer is formed on the magneto-rheological layer, has a piezoresistive effect and can generate resistance change under the stress strain, and a temperature sensing layer is further formed on the electric conduction layer. According to the multifunctional flexible sensing material, the corresponding signal type of the flexible sensing material is extended, so that the flexible sensing material hasgood sensing feedback capacity for magnetism, force and temperature signals, the preparation technology is simple, the circulation stability is good, and the material has high application potential inthe aspect of multi-signal sensing and large-scale production in the field of flexible sensing.

The invention relates to a detection method in the technical field of electrochemistry, specifically relates to an sandwiched electrochemical adapter sensor for quick detecting thrombin by utilizing reduced graphene modified by nanogold-formylimidazole and cobalt-palladium nanoparticles, and belongs to the technical fields of a novel functional material and biosensing analysis. The electrochemical adapter sensor for detection of thrombin has a linear range of 0.010-2.00 ng*ml<-1> and a detection limit of 0.0032 ng*ml<-1>.

It is a process of geophysical prospecting of a porous geological formation (1) containing at least one electrolytic fluid, comprising the following steps: stimulation of a region of interest (90) of the formation where the electrolytic fluid is found with an excitation signal corresponding to an energy of a first type, in such a way that the excitation signal is converted in the region of interest into a response signal corresponding to an energy of a second type, detection of a response signal, application of an electric polarisation to the region of interest (90) so as to strengthen the response signal relative to what it would be in the absence of polarisation.

The invention provides a temperature-controllable electrochemical DNA biosensor and a preparation method thereof. The preparation method comprises fixing a sulfhydrylated single-chain DNA complementary with a target DNA sequence segment to the surface of a gold wire thermode so that a capture probe is obtained, carrying out complementation with the target DNA to obtain a double-chain structure with a flush end 3', and inducing an excision enzyme III to digest the capture probe from the flush end 3' to 5' of the double-chain structure so that the target DNA is released, carries out novel hybridization digestion cycling with other capture probes so that finally, the amount of the capture probes on the surface of the electrode is significantly reduced. Before and after digestion cycling, a reduction degree of electrode surface capture probe electrochemical adsorption signals to hexaamimineruthenium trichloride in the detection liquid is in a linear relationship with the logarithm of a DNA concentration so that the detection of the target DNA can be achieved. The detection method provided by the invention has the characteristics of short detection time, high sensitivity, low detection line and good selectivity to the detection of the target DNA.

The invention relates to a sensor, in particular to a subcutaneous tissue interventional type needle glucose sensor and its preparation method. The sensor comprises a base, the bottom of the base is provided with a working electrode, a counter electrode and a reference electrode, and the working electrode, the counter electrode and the reference electrode form a split needle three-electrode system. The subcutaneous tissue interventional type needle glucose sensor and its preparation method have the advantages that the structure is compact, operating performance is improved and the service life is prolonged.

The invention belongs to the technical field of electrochemical detection, and aims to provide an electrical signal acquisition and transmission device for a flat plate type working electrode. The electrical signal acquisition and transmission device is simple in structure, easy to operate and high in sensitivity, improves the detection effect, and comprises a base, an interface layer, a transmission layer and a top layer; a data transmission interface slot is formed in the left side of the interface layer; three electrode joint positioning holes are formed in the right side of the interface layer; the transmission layer comprises a printed circuit board; three electrode joint welding holes are respectively formed in the printed circuit board; a data transmission interface element is fixed on the printed circuit board; the top ends of three electrode joints are respectively welded at three welding holes; the lower ends of the three electrode joints respectively penetrate three electrode joint positioning holes in the interface layer; the top ends of the three electrode joints are respectively connected with the data transmission interface element through signal transmission lines; the top layer, the transmission layer, the interface layer and the base are sequentially mounted together from top to bottom; the upper end and the lower end of a spring are respectively mounted in an upper spring fixing slot and a lower spring fixing slot.

The invention provides a detection method for metal ions by an amino acid regulated quantum dot fluorescent array sensor. The detection method comprises the following steps: modifying An Mn-doped ZnS quantum dot by using a stabilizer; combining with different amino acids; and adding a to-be-detected metal ion solution for fluorescent detection, wherein the amino acids are glutamine (Gln) and arginine (Arg). The method provided by the invention skillfully combines QDs with the amino acids, can detect various metal ions under the circumference of using two different ligands and is low in detection limit and can differentiate 9 metal ions by 100% under the circumference of 500nM. If the types of the quantum dots are increased (for example 10 types) and the types of the amino acids can be also increased (for example 18 types), the quantity (10*18=180) of the increased sensing units can be easily obtained, so that high-sensitivity detection and recognition on more types of metal ions can be achieved. The invention claims a preparation method of the amino acid regulated quantum dot fluorescent array sensor and use thereof in various metal ion detection.

A fluorescence-based method for highly sensitive and selective detection of analyte molecules is proposed. The method employs the energy transfer between two or more fluorescent chromophores in a carefully selected polymer matrix. In one preferred embodiment, signal amplification has been achieved in the fluorescent sensing of dimethyl methylphosphonate (DMMP) using two dyes, 3-aminofluoranthene (AM) and Nile Red (NR), in a hydrogen bond acidic polymer matrix. The selected polymer matrix quenches the fluorescence of both dyes and shifts dye emission and absorption spectra relative to more inert matrices. Upon DMMP sorption, the AM fluorescence shifts to the red at the same time the NR absorption shifts to the blue, resulting in better band overlap and increased energy transfer between chromophores. In another preferred embodiment, the sensitive material is incorporated into an optical fiber system enabling efficient excitation of the dye and collecting the fluorescent signal form the sensitive material on the remote end of the system. The proposed method can be applied to multichromophore luminescence sensor systems incorporating N-chromophores leading to N-fold signal amplification and improved selectivity. The method can be used in all applications where highly sensitive detection of basic gases, such as dimethyl methylphosphonate (DMMP), Sarin, Soman and other chemical warfare agents having basic properties, is required, including environmental monitoring, chemical industry and medicine.

The invention discloses a CIGS ultra-high and ultra-fast wide-waveband light position sensitive detector, and belongs to the technical field of position detectors. The CIGS ultra-high and ultra-fast wide-waveband light position sensitive detector comprises a photoresponse film layer, a first electrode and a second electrode, wherein the photoresponse film layer grows on a glass substrate; the first electrode and the second electrode are arranged on the photoresponse film layer and connected with a voltmeter or an oscilloscope in series by virtue of a wire; the photoresponse film layer comprises a metal back electrode Mo layer, a CIGS light absorption layer, a CdS transition layer, a ZnO window layer and an ITO transparent conductive layer which are sequentially arranged on the glass substrate; and the first electrode and the second electrode are arranged on the ITO transparent conductive layer. According to the position detector, the effects of a wide-waveband fast response and an ultrahigh signal can be achieved and the sensitivity is high.

The present invention discloses a temperature-controllable electrochemical Hg<2+> sensor based on exonuclease (Exo III) target circulating signal amplification, and a preparation method thereof. The sensor comprises a gold plate thermal electrode, a signal probe P1, an auxiliary probe P2 and exonuclease III, the T-T mismatching structure capable of identifying Hg<2+> can be produced through the complementation of P2 and P1, the T-T mismatching structure and Hg<2+> for a double-stranded structure having a T-Hg<2+>-T structure and having a 3' blunt end, the exonuclease III is induced to digest the P1, the amount of the P1 on the surface of the electrode is decreased so as to decrease the Fc chemical signal, and the decreasing degree of the Fc chemical signal on the electrode surface and the Hg<2+> concentration form linear relationship, such that the high sensitivity detection on the Hg<2+> can be achieved.

The invention relates to a deliquescent-polyelectrolyte-based full-solid-state ethanol gas sensor enzyme electrode and a manufacturing method thereof. The manufacturing method comprises the following steps: on a matrix made of an insulating material, preparing a printed electrode composed of a working electrode, a reference electrode and a counter electrode by screen printing technology; coating a polycarbonate insulator on the surfaces in the middle; coating a reaction layer composed of graphene, deliquescent polyelectrolyte and alcohol oxidase on the working electrode; and coating a deliquescent solid polymer film among the working electrode, counter electrode and reference electrode to obtain the biological enzyme electrode. The invention provides an enzyme electrode for gas-state ethanol detection; and the enzyme electrode can implement high-selectivity detection of ethanol gas without using any liquid electrolyte in the use process, thereby integrally enhancing the properties and practicality of the ethanol gas biosensor.

The invention relates to a three-dimensional array induction calibration device for radial component calibration. The three-dimensional array induction calibration device for the radial component calibration is characterized by comprising two coaxial calibration loops with a certain distance therebetween, a rotating device and a moving device, wherein the whole three-dimensional array induction calibration device moves in the axial direction of a three-dimensional array induction logging instrument through the moving device; the rotating device is used for regulating the azimuth angle of the three-dimensional array induction calibration device; and the three-dimensional array induction calibration device further ensures that the azimuth angle of an axial calibration device is not changed in the calibrating process.

The invention discloses a transition metal carbonitride@cobalt-aluminum hydrotalcite nanosheet / carboxymethyl-beta-cyclodextrin composite membrane modified electrode, and a preparation method thereof and application of the modified electrode in identification and detection of a methionine enantiomer. The preparation method comprises the steps: firstly, preparing MXene by utilizing a fluorate etching method, and growing hydrotalcite-like nanosheets on the surface of the MXene in situ; and modifying carboxymethyl-beta-cyclodextrin to the surface of MXene@LDHNS by utilizing an electrostatic adsorption effect to prepare an MXene@LDHNS / CMCD nano composite material, and preparing a corresponding composite membrane modified electrode by adopting a dispensing method. The modified electrode plays a synergistic role of MXene, hydrotalcite-like nanosheets and carboxymethyl-beta-cyclodextrin, identification and high-sensitivity detection of the methionine enantiomer are realized, the linear range of L-methionine detection is 2*10<-8>-2.5*10<-5> mol / L, and the detection limit is 9.6 nmol / L; and the linear range of D-methionine detection is 1*10<-7>-2.5*10<-5> mol / L, and the detection limit is 39 nmol / L. The preparation method of the modified electrode is simple, and high-sensitivity identification and detection of the methionine chiral enantiomer can be realized.

A fluorescence-based method for highly sensitive and selective detection of analyte molecules is proposed. The method employs the energy transfer between two or more fluorescent chromophores in a carefully selected polymer matrix. In one preferred embodiment, signal amplification has been achieved in the fluorescent sensing of dimethyl methylphosphonate (DMMP) using two dyes, 3-aminofluoranten (AM) and Nile Red (NR), in a hydrogen bond acidic polymer matrix. The selected polymer matrix quenches the fluorescence of both dyes and shifts dye emission and absorption spectra relative to more inert matrices. Upon DMMP sorption, the AM fluorescence shifts to the red at the same time the NR absorption shifts to the blue, resulting in better band overlap and increased energy transfer between chromophores. In another preferred embodiment, the sensitive material is incorporated into an optical fiber system enabling efficient excitation of the dye and collecting the fluorescent signal form the sensitive material on the remote end of the system. The proposed method can be applied to multichromophore luminescence sensor systems incorporating N-chromophores leading to N-fold signal amplification and improved selectivity. The method can be used in all applications where highly sensitive detection of basic gases, such as dimethyl methylphosphonate (DMMP), Sarin, Soman and other chemical warfare agents having basic properties, is required, including environmental monitoring, chemical industry and medicine.

The invention relates to a memory and a preparation method thereof. The memory comprises a semi-conductor substrate, two electrodes arranged on the semi-conductor substrate at an interval and a film layer for enabling the two electrodes to be connected, wherein the film layer is composed of precious metal nano particles clad with conducting polymers. The preparation method of the memory comprises the following steps of (a) coating a layer of photoresist on the semi-conductor substrate in a spinning mode, and photoetching a cavity; (b) depositing metal in the cavity to form the two electrodes at an interval; (c) preparing precious metal nano particle / conducting polymer composite materials; (d) dropping the composite materials diluted by deionized water between the two electrodes at an interval. By means of the film layer connected between the two electrodes and composed of the precious metal nano particles clad with the conducting polymers, the memory is simple in structure, small in size, low in cost and high in integration degree and stability and has high response signals.

The invention relates to a method for detecting the concentrations of drugs in human plasma. According to the method, a sample is pretreated by using a methanol protein precipitation method and then further diluted; and detection is carried out by using an ultra-high performance liquid chromatography-tandem mass spectrometry. The method provided by the invention is capable of detecting three substances such as sacubitril, desethyl sacubitril and valsartan at the same time, so that less damage is caused to chromatographic columns and instruments.

The invention relates to a high performance liquid chromatography-tandem mass spectrometry detection method for vitamin K1 in serum. The method comprises the following steps: sample pretreatment: adding a precipitant into a serum sample to be detected for precipitation; adding an extracting agent for extraction and centrifugation; drying the obtained supernate, redissolving with a redissolving solution, and centrifuging; taking the supernate as a to-be-detected sample solution; the precipitant being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol, an internal standard substance and ethanol; the extraction agent being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol and normal hexane; the reconstitution fluid being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol and methanol; performing liquid chromatography separation: performing chromatographic separation on the to-be-detected sample solution by using high performance liquid chromatography; and performing mass spectrum detection: carrying out mass spectrum detection on the sample separated by the high performance liquid chromatography. The method provided by the invention has the advantages of simple steps, strong specificity, high sensitivity and good anti-interference capability.

The invention discloses a method for evaluating the migration risk of antibiotic drug resistance genes. The method comprises the steps that a first target strain and a second target strain are provided, the first target strain has double fluorescence signals, and the second target strain has multi-antibiotic sensitivity; enabling the first target strain to be in contact with a second target strain in a sample to be detected under a condition suitable for jointing to obtain a zygote; and carrying out fluorescence detection on the zygotes. According to the method, the risk of antibiotic drug-resistant gene migration can be rapidly evaluated, the defect that time and labor are wasted in an existing migration frequency calculation method is effectively overcome, and meanwhile the risk of drug-resistant gene horizontal migration under different environmental conditions is rapidly evaluated. In addition, the double-fluorescence drug-resistant escherichia coli constructed by the invention is used as a donor bacterium for researching the horizontal conjugational transfer of the antibiotic drug-resistant gene, and the drug-resistant gene entering the intestinal tract of a plant or an aquatic organism can be quickly positioned, so that the drug-resistant gene transfer visualization is realized.

The invention belongs to the technical field of electrochemical sensors, and discloses an electrochemical sensor for detecting dopamine in gastric juice as well as a preparation method and application thereof. The preparation method disclosed by the invention comprises the following steps: (1) preparing Nb2C-MXene; (2) preparing NS-Nb2C-MXene; (3) preparing an NS-Nb2C-MXene / Nafion modified electrode, and preparing the NS-Nb2C-MXene / Nafion modified electrode, (4) constructing the electrochemical dopamine sensor based on the NS-Nb2C-MXene / Nafion. Through a simple heteroatom doping preparation method, the nitrogen and sulfur co-doped Nb2C / Nafion modified electrode disclosed by the invention has good electrochemical activity, stability and reproducibility, and nitrogen and sulfur co-doping synergistically enhances an electrochemical response signal of a sensor, so rapid and sensitive detection of dopamine in gastric juice under an acidic condition is realized.

The invention is a sensor, in particular to a subcutaneous tissue-intervening needle-shaped glucose sensor and a preparation method thereof. It includes a base, and the bottom of the base is provided with a working electrode, a counter electrode and a reference electrode, and the working electrode, the counter electrode and the reference electrode form a split needle-shaped three-electrode system. The subcutaneous tissue-intervening needle-like glucose sensor and the preparation method thereof have a compact structure, improved operation performance, and improved service life.

The invention discloses a method for preparing a high-sensitivity optical ammonia sensitive material. The method includes the following steps that 1, a P-shaped silicon slice is fixed into an electrolytic tank, a solution mixed by hydrofluoric acid, ethyl alcohol and glycerol serves as an electrolyte solution, electrochemistry direct-current etching is carried out, etching is carried out for 200 s to 1,200 s in the current of 20 mA / cm<2> to 100 mA / cm<2>, and then washing and drying are carried out; 2, oxidation is carried out for 1 hour to 5 hours at the temperature of 400 DEG to 550 DEG C; 3, acid-base sensitive dye and the ethyl alcohol are mixed, an acetic-acid water solution is added, a carrier of a nanometer porous material is loaded with an acid-base sensitive dye solution, drying is carried out, and the high-sensitivity optical ammonia sensitive material is obtained. According to the ammonia sensitive material, the porous material with the high specific surface area serves as the carrier, the loading capacity of an indicating agent is large, the sensitivity is high, and ammonia with the lowest value of 0.1 ppm can be detected; the response speed is high, the response time is in 10 s, and the method can be applied to online detection; the preparing technology is simple, and industrialization can be achieved.