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A detection method and device of a polymer membrane ion selective electrode

An ion-selective, polymer membrane technology, used in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of cumbersome operation process, and achieve the effect of good selectivity, wide application range and rich variety

Active Publication Date: 2011-11-30
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies such as the cumbersome operation process of ion-selective electrodes in the prior art, the technical problem to be solved by the present invention is to provide a detection method based on a nucleic acid aptamer-based polymer membrane ion-selective electrode that is easy to operate and responds quickly. method and device

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  • A detection method and device of a polymer membrane ion selective electrode
  • A detection method and device of a polymer membrane ion selective electrode
  • A detection method and device of a polymer membrane ion selective electrode

Examples

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Embodiment 1

[0031] like figure 1 As shown, the polymer membrane ion selective electrode detection device of the present invention includes a detection cell 9 , a polycation selective electrode 4 , an internal reference electrode 1 and an external reference electrode 5 . Among them, the detection pool 9 is a container for containing the measured object; the polycation selective electrode 4 is a working electrode, which contains an internal filling liquid 2 and a polymer sensitive film 3 at the bottom; one end of the internal reference electrode 1 is inserted into the Inside the polycation selective electrode 4, the other end is connected to an external potential measuring device; one end of the external reference electrode 5 is set in the detection cell 9, and the other end is connected to the external potential measuring device.

[0032] like figure 2 As shown, the polycation selective electrode 4 in this embodiment can also be connected to the rotating electrode head of the rotating di...

Embodiment 2

[0049] In this embodiment, the determination of potassium ions by titration is an example, and its determination steps are as follows:

[0050] a. 5.0mL pH 7.4 tris buffer solution containing 0.12M sodium chloride, add 2×10 -6 M potassium aptamer, the base sequence of the aptamer is 5'-GGTTGGTGTGGTTGG-3', insert the polycation selective electrode 4 into the above solution, and add 5 μL of 1 mg / mL protamine in sequence every two minutes , to record the potential real-time response;

[0051] b. Add 2×10 -6 M potassium aptamers were combined with 5×10 -6 M, 10 -5 M, 5×10 -5 M, 10 -4 M, 5×10 -4 M potassium ions, after 20 minutes of interaction between the two, insert the polycation selective electrode 4 into the above solution, and add 5 μL of 1 mg / mL protamine successively at intervals of every two minutes, and record the real-time response of the potential, as shown in FIG. Figure 4 shown;

[0052] c. Using the concentration of protamine corresponding to half of the max...

Embodiment 3

[0055] The difference from Example 1 is that this example takes adenosine triphosphate (ATP) aptamer as the substrate of nuclease, and measures the activity of nuclease as an example. The buffer solution is pH 4.6 acetic acid-sodium acetate buffer containing 0.15M sodium chloride and 0.001M zinc sulfate.

[0056] Its measurement steps are as follows:

[0057] a. 3.0mL pH 4.6 acetic acid-sodium acetate buffer solution containing 0.15M sodium chloride and 0.001M zinc sulfate contains 10 -6 M Adenosine triphosphate (ATP) aptamer, the base sequence is 5'-ACCTGGGGGA GTATT GCGGA GGAAG GT-3', after adding 26.7 μg / mL protamine and interacting with the two for 10 minutes, the polycation selective electrode 4 Insert the above solution, record the real-time potential response of the polycation electrode, and use the electrode potential value at 10 minutes as the control signal;

[0058] b. In 3.0mL of the above buffer solution, 1U / mL, 5U / mL, 10U / mL, 30U / mL, 50U / mL of nuclease were mixe...

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Abstract

The invention relates to a method and a device for detecting a polymer film ion selective electrode. The method comprises the following steps of: putting a nucleic acid aptamer and polycation into a detection pond, and inserting a polycation selective electrode into the detection pond to generate a comparison signal; adding the nucleic acid aptamer into the detection tank, reacting with standard solution of a substance to be detected, adding the polycation, and inserting the polycation selective electrode into the detection pond to generate standard signals of the standard solution of the substance to be detected at different concentrations; obtaining a standard curve according to the standard signals and the comparison signal; adding liquid to be detected into the detection pond, adding the nucleic acid aptamer, reacting with the liquid to be detected, adding the polycation in the ratio to conduct the signals, and inserting the polycation selective electrode into the detection pond to generate a sample signal; and acquiring a signal to be detected according to the sample signal and the comparison signal, and acquiring the concentration of the liquid to be detected by the reference of the standard curve. The method is wide in application range, high in generality, large in electrode response signals and obvious in potential change, so the method is a marking-free and immobilization-free method.

Description

technical field [0001] The invention relates to a potential-type electrochemical detection technology, specifically a detection method and a device thereof based on a nucleic acid aptamer-based polymer membrane ion-selective electrode. Background technique [0002] As a new type of molecular recognition element, aptamer can efficiently and specifically bind various ligands, and has the advantages of easy synthesis, easy storage and good stability; however, aptamer itself does not have the function of displaying signals . At present, nucleic acid aptamer-based detection methods usually need to label fluorescent and nanomaterials to generate detection signals. At the same time, detection often requires the immobilization of aptamers, which cannot be detected in a homogeneous solution. On the one hand, this type of detection method has high detection cost and cumbersome operation; on the other hand, the labeling process also affects the binding ability of the nucleic acid apta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403G01N27/333
Inventor 秦伟丁家旺王学伟
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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