Preparation method and application of electrochemical adapter sensor for quick detecting thrombin

An aptamer sensor and thrombin technology, which is applied in the direction of material electrochemical variables, can solve the problems of less thrombin and accurate detection, and achieve the effects of strong catalytic ability, improved sensitivity, and enhanced response signal

Active Publication Date: 2015-12-16
上海佑科仪器仪表有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of thrombin in the human body is very small, and it is difficult to detect it accurately by general detection methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation method of an electrochemical aptasensor for rapid detection of thrombin

[0026] (1) Select thiol-modified thrombin capture probe Apt1 and biotin-modified thrombin reporter probe Apt2; (2) Synthesize gold nanoparticles-modified formyl imidazole-modified reduced graphene AuGS-FI; weigh the mass ratio as 1:1 formyl imidazole modified reduced graphene GS-FI and chloroauric acid, dispersed in water for 24 hours, centrifuged to remove the supernatant, re-dispersed in water, and adjusted the pH-1 NaBH 4 The solution is completely reduced to chloroauric acid, finally, centrifuged and washed three times with ultrapure water, and dried in a vacuum oven;

[0027] (3) Preparation of cobalt palladium nanoparticles CoPdNPs modified thrombin reporter probe CoPdNPs-Apt2

[0028] Add 3 mg of cobalt palladium nanoparticles CoPdNPs with a particle size of about 7 nm to 1.5 mL with a concentration of 0.05 mol L -1 cetyltrimethylammonium bromide CTMAB solution, ul...

Embodiment 2

[0037] Example 2 Preparation method of an electrochemical aptasensor for rapid detection of thrombin

[0038](1) Select thiol-modified thrombin capture probe Apt1 and biotin-modified thrombin reporter probe Apt2; (2) Synthesize gold nanoparticles-modified formyl imidazole-modified reduced graphene AuGS-FI; weigh the mass ratio as 1:1~1.1 formyl imidazole modified reduced graphene GS-FI and chloroauric acid, dispersed in water for 24 hours, centrifuged to remove the supernatant, re-dispersed in water, and adjusted the pH-1 NaBH 4 The solution is completely reduced to chloroauric acid, finally, centrifuged and washed three times with ultrapure water, and dried in a vacuum oven;

[0039] (3) Preparation of cobalt palladium nanoparticles CoPdNPs modified thrombin reporter probe CoPdNPs-Apt2

[0040] Add 4 mg of cobalt palladium nanoparticles CoPdNPs with a particle size of about 8 nm to 2.0 mL with a concentration of 0.05 mol L -1 cetyltrimethylammonium bromide CTMAB solution,...

Embodiment 3

[0049] Example 3 Preparation method of an electrochemical aptasensor for rapid detection of thrombin

[0050] (1) Select thiol-modified thrombin capture probe Apt1 and biotin-modified thrombin reporter probe Apt2; (2) Synthesize gold nanoparticles-modified formyl imidazole-modified reduced graphene AuGS-FI; weigh the mass ratio as 1:1.2 formyl imidazole modified reduced graphene GS-FI and chloroauric acid, dispersed in water for 24 hours, centrifuged to remove the supernatant, re-dispersed in water, and adjusted the pH-1 NaBH 4 The solution is completely reduced to chloroauric acid, finally, centrifuged and washed three times with ultrapure water, and dried in a vacuum oven;

[0051] (3) Preparation of cobalt palladium nanoparticles CoPdNPs modified thrombin reporter probe CoPdNPs-Apt2

[0052] Add 5 mg of cobalt palladium nanoparticles CoPdNPs with a particle size of about 9 nm to 2.5 mL with a concentration of 0.05 mol L -1 cetyltrimethylammonium bromide CTMAB solution, ...

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Abstract

The invention relates to a detection method in the technical field of electrochemistry, specifically relates to an sandwiched electrochemical adapter sensor for quick detecting thrombin by utilizing reduced graphene modified by nanogold-formylimidazole and cobalt-palladium nanoparticles, and belongs to the technical fields of a novel functional material and biosensing analysis. The electrochemical adapter sensor for detection of thrombin has a linear range of 0.010-2.00 ng*ml<-1> and a detection limit of 0.0032 ng*ml<-1>.

Description

technical field [0001] The invention relates to a detection method in the field of electrochemical technology, specifically, a sandwich-type electrochemical aptamer sensor for rapid detection of thrombin is constructed by using gold nano-formyl imidazole modified reduced graphene and cobalt palladium nanoparticles, which belongs to a new type Functional materials and biosensing analysis technology field. Background technique [0002] Thrombin Tb is a proteolytic enzyme formed from the thrombin precursor, which can promote the conversion of fibrinogen into fibrin. It is a serine protein commonly found in mammals. Its increase will lead to the formation of thrombus, while its decrease Can cause bleeding. Clinically, thrombin is suitable for hemostasis of lower blood vessels, capillaries, and substantive organ bleeding that are difficult to stop bleeding. The amount of thrombin in the organism will lead to the occurrence of some diseases. The content of thrombin in the hum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 马洪敏魏琴庞雪辉曹伟罗川南胡丽华杜斌范大伟吴丹张勇
Owner 上海佑科仪器仪表有限公司
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