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Method for manufacturing nano platinum and multiwalled carbon nanotube modified glassy carbon electrode and method for detecting estradiol by utilizing glassy carbon electrode

A technology of multi-walled carbon nanotubes and glassy carbon electrodes, which is applied in the field of glassy carbon electrodes and the detection of estradiol, can solve the problems of difficulty in popularizing grassroots units, expensive instruments, complicated pretreatment, etc., and achieve good electrochemical stability , good application potential, improve the effect of electrochemical response signal

Inactive Publication Date: 2014-12-10
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among these methods, some instruments are expensive, complicated to operate, require complex pre-processing or the assistance of some chemometric algorithms, and it is difficult to achieve popularization in grassroots units.

Method used

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  • Method for manufacturing nano platinum and multiwalled carbon nanotube modified glassy carbon electrode and method for detecting estradiol by utilizing glassy carbon electrode
  • Method for manufacturing nano platinum and multiwalled carbon nanotube modified glassy carbon electrode and method for detecting estradiol by utilizing glassy carbon electrode
  • Method for manufacturing nano platinum and multiwalled carbon nanotube modified glassy carbon electrode and method for detecting estradiol by utilizing glassy carbon electrode

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Multi-walled carbon nanotubes (MWCNTs) were sonicated in 1:1 concentrated sulfuric acid and concentrated nitric acid for 4 hours, the black suspension was filtered, washed with water until neutral, and dried in an oven at 60°C. Then 2 mg of acidified multi-walled carbon nanotubes (MWCNTs) were dispersed in 1 mL of ethylene glycol aqueous solution (the ratio of ethylene glycol to water was 1:1), and sonicated until uniformly dispersed.

[0044] The bare glassy carbon electrode was polished on 0.05 μm alumina powder, and then sonicated in absolute ethanol and water for 1 min sequentially. Rinse with water, then dry with nitrogen. 4 μL of multi-walled carbon nanotube solution (2 mg L -1 ) was drip-coated on the surface of the glassy carbon electrode and dried under infrared light. Afterwards, rinse the electrode surface with water to remove loosely adsorbed MWCNTs and dust, and place the electrode in 1 mmol L -1 Chloroplatinic acid (H 2 PtCl 6 ) aqueous solution, cons...

Embodiment 2

[0046] Multi-walled carbon nanotubes (MWCNTs) were sonicated in 1:1 concentrated sulfuric acid and concentrated nitric acid for 4.5 h, and 10 μL of MWCNTs solution (2 mg L -1 ) is drop-coated on the surface of the glassy carbon electrode, and all the other processes are the same as in Example 1.

Embodiment 3

[0048] Multi-walled carbon nanotubes (MWCNTs) were sonicated in 1:1 concentrated sulfuric acid and concentrated nitric acid for 5 h, and 12 μL of MWCNTs solution (2 mg L -1 ) is drop-coated on the surface of the glassy carbon electrode, and all the other processes are the same as in Example 1. In addition to the above embodiments, the following experiments were carried out on various electrodes:

[0049] To gain insight into the role of each layer of modified material in the electrode response, 1 mmol L -1 Potassium ferricyanide (K 3 Fe(CN) 6 ) (including 0.1mol L -1 KCl supporting electrolyte) solution was used as a probe to study its electrochemical response signals on different electrodes. Such as figure 2 As shown, the cyclic voltammogram overlay compares the bare electrode (GCE), multi-walled carbon nanotube-modified electrode (MWCNTs / GCE), nano-platinum and multi-walled carbon nanotube-modified and glassy carbon electrode (Pt / MWCNTs / GCE) Response to potassium ferr...

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Abstract

The invention discloses a method for manufacturing a nano platinum and multiwalled carbon nanotube modified glassy carbon electrode. The method comprises the following steps: step one, performing ultrasonic treatment on multiwalled carbon nanotubes in an acid solution; step two, dispersing the multi-walled carbon nanotubes processed in the step one in a glycol aqueous solution, and dispersing uniformly through ultrasonic waves to obtain a multi-walled carbon nanotube solution; step three, polishing a bare glassy carbon electrode on alumina powder, then carrying out ultrasonic treatment in absolute ethyl alcohol and water in sequence; step four, dispensing the multi-walled carbon nanotube solution obtained in the step two on the surface of the bare glassy carbon electrode processed in the step three and drying to obtain a multiwalled carbon nanotube modified glassy carbon electrode ; step five, putting the multiwalled carbon nanotube modified glassy carbon electrode processed in the step four in a chloroplatinic acid aqueous solution and carrying out constant potential deposition to obtain the nano platinum and multiwalled carbon nanotube modified glassy carbon electrode. According to the method, the response signal of estradiol can be improved, and the detection reliability for estradiol detection is improved.

Description

technical field [0001] The invention relates to the technical field of estradiol detection in organisms, in particular to a method for manufacturing a glassy carbon electrode modified by nano-platinum and multi-wall carbon nanotubes and using the electrode to detect estradiol. Background technique [0002] Estradiol (17β-estradiol) is an important natural steroidal estrogen (structural formula see figure 1 ), participate in many physiological activities, and play an important role in reproductive function, so it is widely used in treatment. In clinical analysis, the content of estradiol in serum and urine is used as an important indicator for evaluating menopause, hyperandrogenism, breast cancer, etc. In recent years, the use of estradiol in therapy has increased year by year, so that it can also be detected in the ecological environment. However, the excessive use of estradiol will cause many side effects, and low concentration (ppt~ppb) of estradiol will also affect the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/36G01N27/30
Inventor 于岚岚毛烨炫屈凌波
Owner ZHENGZHOU UNIV
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