Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor

An array sensor and detection method technology, applied in the field of optical analysis, can solve the problems of expensive instruments, high cost, cumbersome operation, etc., and achieve the effects of low detection limit, good linearity, and high sensitivity detection and identification

Inactive Publication Date: 2017-05-31
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have some disadvantages, such as relatively high requirements for operating skills, expensive instruments, high cost, cumbersome operation

Method used

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  • Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor
  • Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor
  • Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1: Preparation of Mn-doped ZnS quantum dots capped with mercaptopropionic acid

[0037] Preparation of Mn-ZnS quantum dots (MPA-QD) capped with 3-mercaptopropionic acid: ZnSO 4 ·7H 2 O(7.5mL, 0.1M), Mn(CH 3 COO) 2 ·4H 2 O (0.1 mL, 0.1M) and mercaptopropionic acid MPA (0.26 mL) were added to the three-neck flask. The amount of the mixed solution was made up to 50 mL with ultrapure water, and adjusted to pH 10.3-10.5 with 10M NaOH.

[0038] After removing the air by bubbling argon at room temperature for 30 minutes, the Na 2 S·9H 2 O (7.5mL, 0.1M) was quickly injected into the solution. The mixture was stirred vigorously for 20 minutes and then at 50°C for another 2 hours to form MPA-QDs. For purification, the obtained QDs were precipitated with ethanol and separated by centrifugation (8000 rpm, 5 minutes). This ethanol precipitation-centrifugation process was repeated three times. Finally, the prepared MPA-QDs were dried in vacuum.

Example Embodiment

[0039] Example 2

[0040] The operation of coating Mn-doped ZnS quantum dots with thioglycerol is:

[0041] 1.0M ZnSO 4 ·7H 2 O(5.0mL), 0.1M Mn(CH 3 COO) 2 ·4H 2 O (1.5mL) solution was stirred at room temperature to obtain Mn 2+ Doped quantum dots; then mixed with 1.0M thioglycerol (TG, 20 mL) and added to a three-necked flask. Make up the amount of the mixed solution to 50 mL with ultrapure water, adjust to pH 10.3 with 10M NaOH and use N 2 Bubble for 30 minutes.

[0042] Then Na 2 S·9H 2 Aqueous O solution (1.0 M, 4.5 mL) was quickly injected into the reaction flask, and the mixture was refluxed for 20 hours. After cooling to room temperature, it was separated from the aqueous solution by adding ethanol and centrifugation, and the operation was the same as in Example 1. Wash with ethanol and dry in vacuum at room temperature.

Example Embodiment

[0043] Example 3

[0044] The Mn-doped ZnS QDs coated with thioglycerol (TG) prepared in Example 2 were prepared into a 200μg / mL solution, 210μL was taken out with a pipette tip and added to the centrifuge tube, and then the prepared 800μM Arg was added Solution 105μL, then add 63μL of ultrapure water, and finally add 42μL of 10μM metal ion solution. After shaking on a shaker, take out 200μL of the solution with a pipette and add it to the cuvette. Use Edinburgh FLS-920 transient steady state Fluorescence spectrometer performs fluorescence detection, and the transmitted light intensity is recorded as I (see figure 1 Of S5).

[0045] Detect the transmitted light intensity I of the blank solution when no metal ion solution is added 0 . Record the fluorescence spectrum data, and then use the data processing software (originlab) for processing, such as figure 2 , Using QDs and amino acids, six array units are constructed, and a variety of metal ions are added, which can identify meta...

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Abstract

The invention provides a detection method for metal ions by an amino acid regulated quantum dot fluorescent array sensor. The detection method comprises the following steps: modifying An Mn-doped ZnS quantum dot by using a stabilizer; combining with different amino acids; and adding a to-be-detected metal ion solution for fluorescent detection, wherein the amino acids are glutamine (Gln) and arginine (Arg). The method provided by the invention skillfully combines QDs with the amino acids, can detect various metal ions under the circumference of using two different ligands and is low in detection limit and can differentiate 9 metal ions by 100% under the circumference of 500nM. If the types of the quantum dots are increased (for example 10 types) and the types of the amino acids can be also increased (for example 18 types), the quantity (10*18=180) of the increased sensing units can be easily obtained, so that high-sensitivity detection and recognition on more types of metal ions can be achieved. The invention claims a preparation method of the amino acid regulated quantum dot fluorescent array sensor and use thereof in various metal ion detection.

Description

technical field [0001] The invention belongs to the field of optical analysis, and in particular relates to a fluorescence analysis method based on quantum dots. Background technique [0002] At present, the commonly used metal ion detection and analysis methods mainly include: atomic emission method, atomic absorption method, inductively coupled plasma method and so on. These methods have some disadvantages, such as relatively high requirements for operating skills, expensive instruments, high cost, cumbersome operation, inability to distinguish multivalent metal ions, and relatively high professional technical requirements for operators. , is also more time-consuming. [0003] Quantum dots (Quantum dots, QDs) are spherical crystals with a diameter of 1-10 nm composed of II-VI or III-V elements. Compared with traditional organic fluorescent dyes or lanthanide complexes, fluorescent quantum dots It has the following optical properties: (1) The emission wavelength of quantu...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 刘月英景文杰王飞扬贺刘莹杨广才
Owner CAPITAL NORMAL UNIVERSITY
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