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Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor

An array sensor and detection method technology, applied in the field of optical analysis, can solve the problems of expensive instruments, high cost, cumbersome operation, etc., and achieve the effects of low detection limit, good linearity, and high sensitivity detection and identification

Inactive Publication Date: 2017-05-31
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have some disadvantages, such as relatively high requirements for operating skills, expensive instruments, high cost, cumbersome operation, inability to distinguish multivalent metal ions, and relatively high professional technical requirements for operators. , is also more time-consuming

Method used

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  • Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor
  • Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor
  • Detection method for metal ions by amino acid regulated quantum dot fluorescent array sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of Mn-doped ZnS quantum dots capped with mercaptopropionic acid

[0037] Preparation of 3-mercaptopropionic acid-capped Mn-ZnS quantum dots (MPA-QDs): ZnSO 4 ·7H 2 O (7.5mL, 0.1M), Mn(CH 3 COO) 2 4H 2 O (0.1 mL, 0.1 M) and mercaptopropionic acid MPA (0.26 mL) were added to a three-necked flask. The volume of the mixed solution was made up to 50 mL with ultrapure water, and adjusted to pH 10.3-10.5 with 10 M NaOH.

[0038] After removing the air by bubbling argon for 30 min at room temperature, the Na 2 S·9H 2O (7.5 mL, 0.1 M) was quickly injected into the solution. The mixture was stirred vigorously for 20 min and then for another 2 h at 50 °C to form MPA-QDs. For purification, the obtained QDs were precipitated with ethanol and separated by centrifugation (8000 rpm, 5 min). This ethanol precipitation-centrifugation process was repeated three times. Finally, the prepared MPA-QDs were dried in vacuum.

Embodiment 2

[0040] The operation of coating Mn-doped ZnS quantum dots with thioglycerol is:

[0041] 1.0M ZnSO 4 ·7H 2 O (5.0mL), 0.1M Mn(CH 3 COO) 2 4H 2 A solution of O (1.5 mL) was stirred at room temperature to obtain Mn 2+ Doped quantum dots; then mixed with 1.0M thioglycerol (TG, 20mL) and added to a three-necked flask. The volume of the mixed solution was replenished to 50 mL with ultrapure water, adjusted to pH 10.3 with 10 M NaOH and washed with N 2 Bubble for 30 minutes.

[0042] Then Na 2 S·9H 2 O aqueous solution (1.0 M, 4.5 mL) was quickly injected into the reaction flask, and the mixture was refluxed for 20 hours. After cooling to room temperature, it was separated from the aqueous solution by adding ethanol and by centrifugation, and the operation was the same as in Example 1. Wash with ethanol and dry under vacuum at room temperature.

Embodiment 3

[0044] Prepare the Mn-doped ZnS QDs coated with thioglycerol (TG) prepared in Example 2 to make a 200 μg / mL solution, take out 210 μL with a pipette tip and add it to a centrifuge tube, and then add the prepared 800 μM Arg Add 105 μL of the solution, then add 63 μL of ultrapure water, and finally add 42 μL of 10 μM metal ion solution, shake well on the shaker, take out 200 μL of the solution with a pipette tip and add it to a cuvette, using Edinburgh FLS-920 transient steady state The fluorescence spectrometer carries out fluorescence detection, and the transmitted light intensity is recorded as I (see figure 1 S5).

[0045] Detect the transmitted light intensity I of the blank solution when no metal ion solution is added 0 . Record the fluorescence spectrum data, and then use the data processing software (originlab) to process, such as figure 2 , using QDs and amino acids to construct six array units, adding a variety of metal ions, which can efficiently and quickly ident...

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Abstract

The invention provides a detection method for metal ions by an amino acid regulated quantum dot fluorescent array sensor. The detection method comprises the following steps: modifying An Mn-doped ZnS quantum dot by using a stabilizer; combining with different amino acids; and adding a to-be-detected metal ion solution for fluorescent detection, wherein the amino acids are glutamine (Gln) and arginine (Arg). The method provided by the invention skillfully combines QDs with the amino acids, can detect various metal ions under the circumference of using two different ligands and is low in detection limit and can differentiate 9 metal ions by 100% under the circumference of 500nM. If the types of the quantum dots are increased (for example 10 types) and the types of the amino acids can be also increased (for example 18 types), the quantity (10*18=180) of the increased sensing units can be easily obtained, so that high-sensitivity detection and recognition on more types of metal ions can be achieved. The invention claims a preparation method of the amino acid regulated quantum dot fluorescent array sensor and use thereof in various metal ion detection.

Description

technical field [0001] The invention belongs to the field of optical analysis, and in particular relates to a fluorescence analysis method based on quantum dots. Background technique [0002] At present, the commonly used metal ion detection and analysis methods mainly include: atomic emission method, atomic absorption method, inductively coupled plasma method and so on. These methods have some disadvantages, such as relatively high requirements for operating skills, expensive instruments, high cost, cumbersome operation, inability to distinguish multivalent metal ions, and relatively high professional technical requirements for operators. , is also more time-consuming. [0003] Quantum dots (Quantum dots, QDs) are spherical crystals with a diameter of 1-10 nm composed of II-VI or III-V elements. Compared with traditional organic fluorescent dyes or lanthanide complexes, fluorescent quantum dots It has the following optical properties: (1) The emission wavelength of quantu...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 刘月英景文杰王飞扬贺刘莹杨广才
Owner CAPITAL NORMAL UNIVERSITY
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