Preparation method for nano-material used for detection of glucose

A technology of nanomaterials and glucose, which is applied in material analysis by observing the influence of chemical indicators, analysis by making materials undergo chemical reactions, color/spectral characteristic measurement, etc., can solve the problems of easy inactivation, degradation, and easy decomposition , Hydrogen peroxide instability and other problems, to improve the signal strength and detection sensitivity, simplify the experimental operation steps, avoid the effect of diffusion and decomposition

Active Publication Date: 2014-11-19
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some defects in this traditional glucose sensor. First, both glucose oxidase and horseradish peroxidase belong to biological enzymes. They are unstable at room temperature and are easy to inactivate and degrade, so this sensor cannot be stored for a long time; Second, this method essentially uses hydrogen peroxide as an intermediary to produce a color change reaction, but hydrogen peroxide is unstable and easily decomposed in the environment
Therefore, the signal strength (degree of discoloration) and sensitivity of this method are not high

Method used

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  • Preparation method for nano-material used for detection of glucose

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0024] (1) Prepare a 120 mM copper sulfate aqueous solution using an analytical balance and a volumetric flask.

[0025] (2) Weigh 1 mg of glucose oxidase (GOx) derived from Aspergillus niger and 1 mg of peroxidase (HRP) derived from horseradish, and add them to 150 mM phosphate buffer solution (PBS, pH=7). The bottle was adjusted to 10 mL to obtain a PBS solution containing 0.5 mg / mL GOx and 0.1 mg / mL HRP.

[0026] (3) Add 20 μL of 120 mM copper sulfate solution to 3 mL of PBS solution containing 0.5 mg / mL GOx and 0.1 mg / mL HRP, mix the solution well, and place it at room temperature for 3 days. The resulting precipitated substance is GOx&HRP–Cu 3 (PO 4 ) 2 ·3H 2 O Nanoflower.

Embodiment 2

[0028] (1) Use an analytical balance and a volumetric flask to prepare a 100 mM ferric chloride aqueous solution.

[0029] (2) Weigh 5 mg of glucose oxidase (GOx) from Aspergillus niger and 1 mg of soybean peroxidase (HRP), add them to 200 mM phosphate buffer solution (PBS, pH=7), use a volumetric flask Dilute to 10 mL to obtain a PBS solution containing 0.5 mg / mL GOx and 0.1 mg / mL HRP.

[0030] (3) Add 15 μL of 120 mM copper sulfate solution to 3 mL of PBS solution containing 0.5 mg / mL GOx and 0.1 mg / mL HRP, mix the solution well, and place it at room temperature for 3 days. The resulting precipitated substance is the nanoflower.

Embodiment 3

[0032] (1) Prepare a 150 mM calcium chloride aqueous solution using an analytical balance and a volumetric flask.

[0033] (2) Weigh 1mg of glucose oxidase (GOx) from Penicillium and 2mg of peroxidase (HRP) from black tube fungus, add to 250mM phosphate buffer solution (PBS, pH=7), use The volumetric flask was adjusted to 10 mL to obtain a PBS solution containing 0.5 mg / mL GOx and 0.1 mg / mL HRP.

[0034] (3) Add 60 μL of 120 mM copper sulfate solution into 3 mL of PBS solution containing 0.5 mg / mL GOx and 0.1 mg / mL HRP. The solution was mixed well and left at room temperature for 3 days. The resulting precipitated substance is the nanoflower.

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Abstract

The invention discloses a preparation method for a nano-material used for detection of glucose. The method comprises the following steps: first, a metal salt water solution with a concentration of 100-200mM is prepared; second, glucose oxidase and peroxidase are mixed and a buffer solution is prepared; third, the solution obtained in the first step and the solution obtained in the second step are mixed at a volume rate of 1:150; fourth, the mixed solution obtained in the third step is mixed uniformly, and the sediment obtained after standing is the nano-material. Through the method, hybrid nanoflowers with high catalytic activity can be synthesized simply and efficiently, experiment operation steps are simplified, and the response signal of a glucose colorimetric detection system to glucose is raised greatly.

Description

technical field [0001] The invention relates to the field of nanomaterial preparation, in particular to a method for preparing a polyprotein / phosphate nanomaterial for detecting glucose. Background technique [0002] Diabetes is currently one of the most common non-communicable diseases, and it is considered the third most dangerous disease in the world, seriously threatening human health. Generally speaking, the classic colorimetric glucose sensor is based on glucose oxidase (GOx) catalyzing the oxidation of glucose to produce hydrogen peroxide, and the latter oxidizes some chromogenic substrates such as 3,3 under the catalysis of horseradish peroxidase. ',5,5'-Tetramethylbenzidine (TMB) produces a color change for glucose detection. The detection steps are generally divided into two steps: first, glucose reacts with glucose oxidase and oxygen at 37°C, and glucose produces gluconic acid and hydrogen peroxide; Mix a solution of a biochemical enzyme and a chromogenic substr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/78
Inventor 汪鹏飞孙嘉宇葛介超刘卫敏张洪艳
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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