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High performance liquid chromatography-tandem mass spectrometry detection method for vitamin K1 in serum

A technology of high performance liquid chromatography and detection method, which is applied in the field of high performance liquid chromatography tandem mass spectrometry detection of vitamin K1 in serum, which can solve the problems of complex processing process, matrix interference and low sensitivity, and achieve high ionization efficiency and enhanced stability The effect of high sensitivity and sensitivity

Pending Publication Date: 2021-10-26
GUANGZHOU KINGMED DIAGNOSTICS CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still problems such as complicated pretreatment process, serious matrix interference, and low sensitivity.

Method used

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  • High performance liquid chromatography-tandem mass spectrometry detection method for vitamin K1 in serum
  • High performance liquid chromatography-tandem mass spectrometry detection method for vitamin K1 in serum
  • High performance liquid chromatography-tandem mass spectrometry detection method for vitamin K1 in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] (1) Solution preparation:

[0061] Preparation of estriol stock solution: Weigh a certain amount of estriol solid and dissolve it in absolute ethanol to prepare an estriol stock solution with a concentration of 1.0 mg / mL.

[0062] Preparation of internal standard intermediate solution: dilute vitamin K with absolute ethanol 1 Internal standard (D 7 -VK 1 ) stock solution to obtain an internal standard intermediate solution with a concentration of 10.0 μg / mL.

[0063] Preparation of precipitant: pipette 40μL-80μL internal standard intermediate solution and 400μL-800μL estriol stock solution into a 500mL volumetric flask, then add 0.1g-0.5g BHT, and dilute with absolute ethanol to obtain a precipitant.

[0064] Extractant preparation: draw 400μL-800μL estriol stock solution to a 500mL volumetric flask, then add 0.1g-0.5g BHT, and dilute to the volume with n-hexane to obtain the extractant.

[0065] Preparation of complex solution: Add 0.1g-0.5g BHT and 400μL-800μL est...

Embodiment 2

[0103] Example 2 Optimization of sample pretreatment process

[0104] VK 1 It is unstable under light conditions and easily decomposed under ultraviolet light and alkaline conditions. At the same time due to VK 1 It has strong fat solubility and is easily adsorbed on the consumables (centrifuge tubes, sampling bottles, etc.) used in the pretreatment process. Therefore, reducing the VK during preprocessing 1 In the loss, it is very critical to obtain accurate quantitative results and improve sensitivity. In the present invention, by adding a protective agent (antioxidant BHT) to the reagent in the forward treatment process, the degradation caused by light instability is reduced. At the same time to avoid VK 1 Adsorbed by plastic centrifuge tubes or glass bottles, steroid hormones (estriol) are also added to protect the target to be tested through competitive adsorption.

[0105] In the pretreatment process of the same sample, whether the protective agent (BHT and estriol)...

Embodiment 3

[0107] Example 3 Stability test after sample treatment

[0108] Because of VK 1 Instability to light sensitivity, stability during and after processing is of particular importance. Collect patient samples and mix them into mixed serum with different concentrations (concentrations around 6.9ng / mL, 0.8ng / mL), add protective agent in the pretreatment process according to the optimized method, and mix high and low mixed serum samples in parallel Treat 10 parts, mix the treated samples with high concentration and low concentration to be tested respectively, and then divide them into 8 parts with brown glass sampling bottles, store them in the sample tray (15°C), store them at 0, 2, 4, 8, 12, 24, 36, and 48 hours were measured three times each to verify the storage stability of the sample after treatment; the 0-hour test results were used as the reference target value, and the test results at other different storage time points were compared with the 0-hour results. The results in...

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Abstract

The invention relates to a high performance liquid chromatography-tandem mass spectrometry detection method for vitamin K1 in serum. The method comprises the following steps: sample pretreatment: adding a precipitant into a serum sample to be detected for precipitation; adding an extracting agent for extraction and centrifugation; drying the obtained supernate, redissolving with a redissolving solution, and centrifuging; taking the supernate as a to-be-detected sample solution; the precipitant being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol, an internal standard substance and ethanol; the extraction agent being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol and normal hexane; the reconstitution fluid being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol and methanol; performing liquid chromatography separation: performing chromatographic separation on the to-be-detected sample solution by using high performance liquid chromatography; and performing mass spectrum detection: carrying out mass spectrum detection on the sample separated by the high performance liquid chromatography. The method provided by the invention has the advantages of simple steps, strong specificity, high sensitivity and good anti-interference capability.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to a vitamin K in serum 1 High performance liquid chromatography tandem mass spectrometry detection method. Background technique [0002] Vitamin K 1 (VK 1 ), also known as menadione, is a fat-soluble vitamin with relatively stable chemical properties, acid and heat resistance, but sensitive to light, and easily decomposed by alkali and ultraviolet rays. VK 1 It is an essential substance in the process of synthesis of coagulation factors in the liver. When it is deficient, it will affect the synthesis of coagulation factors II, VII, IX, and X, resulting in reduced formation of prothrombin complex and spontaneous bleeding, which has a potentially fatal risk. VK 1 Status can be used to assess malabsorption diseases such as hemorrhagic disease of the newborn, celiac disease, Crohn's disease, ulcerative colitis and chronic pancreatitis (cystic fibrosis), and it has als...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N2030/062Y02A50/30
Inventor 佘旭辉蔡国杰蔡娇赵蓓蓓余木俊董衡程雅婷
Owner GUANGZHOU KINGMED DIAGNOSTICS CENT
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