Visual optic sensing method for detecting target-mediating agglutination reaction of nano-gold probes on the basis of Tyndall effect

A nano-gold probe, agglutination reaction technology, applied in the field of nano-sensing, can solve the problems of high analysis cost, limited detection lower limit, limited red-blue color change sensitivity, etc., to achieve high analytical sensitivity, improved sensitivity, low-cost portable The effect of quantitative detection

Inactive Publication Date: 2018-06-29
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, due to the limited sensitivity of visually observing the red-blue color change of the gold nanoprobe solution during the agglutination reaction, the detection limit of the target object based on the optical qualitative analysis or semi-quantitative analysis of this type of probe is often limited to micro molar level
In addition, most of the existing nano-gold liquid phase optical sensing methods need to use expensive and lack of portability UV-Vis spectrophotometer for quantitative detection, the analysis cost is high, and it is not suitable for on-site analysis and instant inspection applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Visual detection of cysteamine based on Tyndall effect and agglutination reaction of bare gold nanoprobes.

[0022] The specific implementation process is as follows:

[0023] (1) In a 1.5 mL test tube, mix 900 μL of cysteamine sample solution to be tested with 100 μL of colorless gold nano-gold solution (particle size about 15 nm, The stock solution is obtained by reducing chloroauric acid with sodium citrate, and the surface of the prepared gold nanoparticles is negatively charged due to the adsorption of citrate anions), and the cysteamine molecule is positively charged after protonation, and the surface is connected to the surface through electrostatic adsorption. The negatively charged gold nanoprobes bind and induce the agglutination reaction of the latter.

[0024] (2) Use the red laser beam generated by the laser pointer to irradiate the mixture solution obtained in step (1) to generate a Tyndall effect optical signal, and use a mobile phone to record the gray ...

Embodiment 2

[0027] Visual detection of human IgG protein based on Tyndall effect and agglutination reaction of monoclonal antibody-modified gold nanoprobes.

[0028] The specific implementation process is as follows:

[0029] (1) In a 1.5 mL test tube, mix 900 μL of human IgG protein sample solution to be tested with 100 μL of buffer solution (prepared with sodium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with a concentration of 10 mmol / L, pH 7) and dilute 1000 times The IgG protein monoclonal antibody modified functionalized nano-gold probe (particle size is about 15 nm, the stock solution is prepared by reducing chloroauric acid with sodium citrate, and the surface of the prepared gold nano-particles is negative due to the adsorption of citrate anions. Charged) colorless solution.

[0030] (2) Use the red laser beam generated by the laser pointer to irradiate the solution obtained in step (1) to generate a Tyndall effect optical signal, and use a mobile phone ...

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Abstract

The invention discloses a visual optic sensing method for detecting a target-mediating agglutination reaction of nano-gold probes on the basis of Tyndall effect. In the method, by means of an analyticsubstance in a sample solution, a reaction solution, which contains a target capable of mediating the agglutination reaction of naked nano-gold probes or biofunctionalized composite nano-gold probes,can be obtained; then the reaction solution is mixed and reacted with a nano-gold probe solution in order to observe an optical signal of the Tyndall effect of the mixed solution under laser beam formed by a laser pen or a laser device, wherein the signal intensity is in positive correlation with the concentration of the analytic substance. Through a cheap portable device for reading the signal (i.e. the optical signal of the Tyndall effect), low-cost portable quantitative detection of the analytic substance is achieved. The method has simple operations and low cost, is free of special analytic instruments and equipment and is suitable for on-site analysis and real-time detection, and can be directly promoted in the fields such as medical diagnosis, environment monitoring, food safety andthe like.

Description

technical field [0001] The invention belongs to the technical field of nano-sensing, and in particular relates to a visual optical sensing method based on a Tyndall effect detection target-mediated agglutination reaction of a nano-gold probe. Background technique [0002] Nanogold is one of the most common nanometal colorimetric probes. The liquid-phase optical sensing method based on nano-gold probes is simple to operate and low in analysis cost. In particular, the analyte-induced agglutination reaction of nano-gold probes can change the color of the reaction solution from red to blue, thereby realizing simple and rapid visual qualitative or semi-quantitative analysis of analytes. Due to its many advantages, nano-gold liquid phase optical sensing method has been widely used in medical diagnosis, environmental monitoring and food safety and other fields. However, due to the limited sensitivity of visually observing the red-blue color change of the gold nanoprobe solution d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/33G01N21/78
Inventor 聂瑾芳王兵月张云肖文成
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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