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Preparation method of electrochemical sensor for detecting molecular imprinting of organophosphorus pesticide residue

An organophosphorus pesticide and molecular imprinting technology, applied in the field of pesticide detection, can solve the problems of unstable and easy decomposition of organophosphorus pesticides, acute poisoning, elevated blood pressure, etc.

Inactive Publication Date: 2012-03-28
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organophosphorus pesticides are unstable and easy to decompose in the natural environment. After entering the organism, they are easily decomposed by enzymes. They remain in food for a short time and are not easy to cause chronic poisoning, but they can often cause acute poisoning. In severe cases, they can cause muscle tremors, spasms, Elevated blood pressure, rapid heartbeat, etc., even lead to coma and death. Therefore, the detection and control of organophosphorus pesticide residues in agricultural products is directly related to food safety and is closely related to people's health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Choose a glassy carbon electrode as the electrode, and polish it with 0.05 micron-0.30 micron α-alumina slurry; use redistilled ethanol, acetone, and water to clean the electrode respectively; insert the treated electrode into 0.1mol / L KCl and 5mol / L K 3 [Fe(CN) 6 ] in the mixed solution; the stability of the electrode is detected by electrochemical cyclic voltammetry between 0-0.5V, and the electrode is cleaned with high-purity water;

[0041] (2) select o-aminophenol as functional monomer, dissolve it in HClO 4 In the solution, the concentration of o-aminophenol is 0.006mol / L, and the pH value of the solution is adjusted to 5 with the NaOH solution of 1mol / L;

[0042] (3) Select dichlorvos as the imprinted molecule, dissolve it in the monomer solution, and control its concentration to 0.05mol / L, so that the molar ratio of the functional monomer to the imprinted molecule is 5:1;

[0043] (4) The nitrogen gas flow time is 10 minutes, and the airtight shading is...

Embodiment 2

[0047] (1) Choose a glassy carbon electrode as the electrode, and polish it with 0.05 micron-0.30 micron α-alumina slurry; use redistilled ethanol, acetone, and water to clean the electrode respectively; insert the treated electrode into 0.1mol / L KCl and 5mol / L K 3 [Fe(CN) 6 ] in the mixed solution; the stability of the electrode is detected by electrochemical cyclic voltammetry between 0-0.5V, and the electrode is cleaned with high-purity water;

[0048] (2) select o-aminophenol as functional monomer, dissolve it in HClO 4 In the solution, the concentration of o-aminophenol is 0.005mol / L, and the pH value of the solution is 4 with the NaOH solution of 1mol / L;

[0049] (3) Select dichlorvos as the imprinted molecule, dissolve it in the monomer solution, and control its concentration to 0.02mol / L, so that the molar ratio of the functional monomer to the imprinted molecule is 4:1;

[0050] (4) The nitrogen gas flow time is 10 minutes, and the airtight shading is allowed to ...

Embodiment 3

[0054] (1) Choose a glassy carbon electrode as the electrode, and polish it with 0.05 micron-0.30 micron α-alumina slurry; use redistilled ethanol, acetone, and water to clean the electrode respectively; insert the treated electrode into 0.1mol / L KCl and 5mol / L K 3 [Fe(CN) 6 ] in the mixed solution; the stability of the electrode is detected by electrochemical cyclic voltammetry between 0-0.5V, and the electrode is cleaned with high-purity water;

[0055] (2) select o-aminophenol as functional monomer, dissolve it in HClO 4 In the solution, the concentration of o-aminophenol is 0.01mol / L, and the pH value of the solution is 6 with the NaOH solution of 1mol / L;

[0056] (3) Select dichlorvos as the imprinted molecule, dissolve it in the monomer solution, and control its concentration to be 0.08mol / L, so that the molar ratio of the functional monomer to the imprinted molecule is 6:1;

[0057] (4) The nitrogen gas flow time is 10 minutes, and the airtight shading is allowed t...

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PUM

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Abstract

The invention discloses a preparation method of an electrochemical sensor for detecting molecular imprinting of organophosphorus pesticide residue. The method comprises the following steps of: firstly preparing a solution from the raw molecules of organophosphorus pesticide serving as imprinting molecules and a functional monomer and an electrolyte; depositing the molecular imprinting polymer on the surface of a working electrode by an electrochemical polymerization method so as to finish the preparation of a molecular imprinting sensitive film; and removing the imprinting molecules of the organophosphorus pesticide from the molecular imprinting sensitive film by a proper elution method and a proper eluent so as to finish the preparation of the electrochemical sensor. The method disclosed by the invention has the advantages of fast response, high sensitivity and good selectivity; and meanwhile, the detection method is simple and quick, has strong adaptability after being popularized and particularly strong universality in pesticide detection, and has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection and relates to a preparation method of an electrochemical sensor, in particular to a preparation method of an electrochemical sensor for detecting molecular imprints of organophosphorus pesticide residues. Background technique [0002] Organophosphorus pesticides are high-efficiency broad-spectrum insecticides with neurotoxicity. More than 60 kinds are commonly used, such as dimethoate, trichlorfon, dichlorvos, demeton, parathion, and malathion. Organophosphorus pesticides are unstable and easy to decompose in the natural environment. After entering the organism, they are easily decomposed by enzymes. They remain in food for a short time and are not easy to cause chronic poisoning, but they can often cause acute poisoning. In severe cases, they can cause muscle tremors, spasms, Elevated blood pressure, rapid heartbeat, etc., even lead to coma and death. Therefore, the detection and con...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 杨黎燕牛秀明余丽丽上官小东赵惠茹尤静张彦张雪娇姚琳
Owner XIAN MEDICAL UNIV
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