Mercury ion electric sensing analysis and detection method based on excision enzyme III assisted target circulation and subject and object recognition

Abstract

The invention discloses a mercury ion electric sensing analysis and detection method based on excision enzyme III assisted target circulation and subject and object recognition. The mercury ion electric sensing analysis and detection method comprises the following steps that a glassy carbon electrode is polished, ground and cleaned; nanogold is subjected to electrolytic deposition on the surface of the glassy carbon electrode, and thus a nanogold modified electrode is prepared; aminated beta-cyclodextrin is modified on nanogold particles to prepare a beta-cyclodextrin modified electrode, Hg<2+> is added into a mixed solution of DNA A1 and DNA A2 to form a double-chain solution; ferrocene marked DNA A3 is added into the double-chain solution to be incubated, and thus a T-shaped DNA structure is formed; exonuclease III is added into the T-shaped DNA structure to form a mixed solution; the mixed solution is dropwise added to the surface of the beta-cyclodextrin modified electrode; the modified electrode is immersed into a testing base solution to detect the electrochemical properties of the modified electrode, and quantitative measurement of Hg<2+> is achieved according an electrochemical signal; and the linearity and the selectivity of an electrochemical biological sensor are tested.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a mercury ion electrosensing analysis and detection method based on exonuclease III assisting target circulation and host-guest identification. Background technique [0002] The advantages and disadvantages of metals to the human body or the environment not only depend on the type and physical and chemical properties of the metal, but also the concentration of the metal in nature and its chemical form play a decisive role. Even if the content of beneficial metal elements exceeds the standard value, it will cause Body poisoning, and even death. Once the heavy metal ions are absorbed by the body, they will have a huge interaction with the proteins and enzymes in the body. Trace amounts of heavy metal ions will not cause harm to the human body, but if the concentration of heavy metal ions in the body is too high, it will change the three-dimensional structure of the en...

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Problems solved by technology

Although electrochemical anodic stripping voltammetry has the advantages of high sensitivity, no need for high-pressure gas, flame, and atomic lamp, simple experiment, and low cost, the operation of anodic stripping voltammetry requires professional personnel to operate, but mercury is used as an electrode. The environment brings new heavy metal pollution

Spectrophotometry, etc., the detection cost of this method is low, but the sensitivity is not high, can not meet the detection requirements of national standards; and atomic absorption spectrometry, inductively coupled plasma (ICP) method, etc. have low detection limit, high sensitivity and good selectivity , but the price is expensive, professional technicians are required, and the detection process is relatively laborious and time-consuming, etc.

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