Cytochrome C based two-channel ratio type hydrogen peroxide biosensor, and preparation method and application thereof

A biosensor, hydrogen peroxide technology, applied in the direction of material analysis, instruments, and scientific instruments by electromagnetic means, can solve the problems of difficult acquisition, inaccurate results, poor stability, etc., and achieve good electrocatalytic effect and conduction efficiency. Good and stable effect

Inactive Publication Date: 2019-07-30
SHANGHAI INST OF QUALITY INSPECTION & TECHN RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing ratiometric biosensors usually require the preparation of nano-modified electrodes to achieve electron transfer through nano-modified electrodes; moreover, the nano-modified electrodes also need to be synthesized by other mechanisms
Therefore, its preparation method is very complicated and cumbersome, and it is difficult to obtain
In addition, the existing ratiometric biosensors cannot be modified on the electrode surface due to lack of suitable reference substances, resulting in unstable chemical properties, inability to generate response electrical signals in the system, and no response to hydrogen peroxide, etc. The electrocatalytic effect of the sensor is poor, the stability is poor, the result is inaccurate, etc.

Method used

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  • Cytochrome C based two-channel ratio type hydrogen peroxide biosensor, and preparation method and application thereof
  • Cytochrome C based two-channel ratio type hydrogen peroxide biosensor, and preparation method and application thereof
  • Cytochrome C based two-channel ratio type hydrogen peroxide biosensor, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of dual-channel ratiometric hydrogen peroxide biosensor based on cytochrome C

[0037] Before the experiment, the gold electrode was polished on metallographic sandpaper, ultrasonically cleaned with 1:1 ethanol, 1:1 nitric acid and deionized water in sequence, and dried for later use.

[0038] Step 1. Soak the Au electrode (electrode ①) into a 1mmol / L MPA solution, take it out after 10 minutes, and wash it with deionized water to obtain an Au / MPA electrode;

[0039] Step 2. Mix and react EDC (5mmol / L) and NHS (2mmol / L) for 5 hours, adjust the pH to neutral, add Cyt.C solution with a concentration of 5mg / mL, and soak the Au / MPA electrode into the mixture After reacting at room temperature for 1 hour, take it out, wash off the EDC-NHS residue, dry it for later use, and obtain an Au / MPA / Cyt.C electrode (such as figure 2 shown);

[0040] Step 3. Soak the other Au electrode in the FcHT-ethanol solution with a volume ratio of 1:2000, store it away from ...

Embodiment 2

[0041] Modified Characterization, Electrochemical Performance and Stability of Example 2 Au / MPA / Cyt.C Electrode and Au / FcHT Electrode

[0042] (1) Modification and characterization of Au / MPA / Cyt.C electrode and Au / FcHT electrode

[0043] The Au / MPA / Cyt.C electrode and Au / FcHT of Example 1 were used as the working electrode and the reference channel electrode, the Ag / AgCl electrode was used as the reference electrode, and the Pt wire was used as the counter electrode to construct a three-electrode system. The result is as Figure 3A with Figure 3B shown.

[0044] Figure 3A The results showed that only charging current was observed on the Au electrode and Au / MPA electrode, but a pair of clear redox peaks could be observed on the Au / MPA / Cyt.C electrode, indicating that Cyt.C had been successfully modified on the electrode;

[0045] Figure 3B The results showed that only charging current was observed on the Au electrode, while a pair of clear redox peaks could be observed ...

Embodiment 3

[0050] Embodiment 3 electrocatalytic performance

[0051] Such as figure 1 As shown, the prepared Au / MPA / Cyt.C and Au / FcHT electrodes were respectively connected to the CHI 760E electrochemical workstation, and a four-electrode system was formed with the Ag / AgCl electrode and the platinum wire counter electrode, and the differential pulse voltammetry was selected. (DPV) was measured. The initial potential of Au / MPA / Cyt.C is 0.4V, the final potential is -0.1V, the initial potential of Au / FcHT is 0.7V, the final potential is 0.1V, the potential increment is 0.004V, and the test solution is 0.1mol / L PBS solution, pH=7.0. The result is as Image 6 shown.

[0052] Depend on Image 6 It can be seen that with H 2 o 2 With the increase of the concentration, the reduction peak current of Cyt.C increased continuously; while the peak current of the reference peak FcHT remained basically the same. Correlation coefficient R of the ratio of Cyt.C response peak current to FcHT refere...

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Abstract

The invention belongs to the technical field of biosensor detection, and discloses a cytochrome C based two-channel ratio type hydrogen peroxide biosensor, comprising an Au / MPA / Cyt. C electrode and anAu / FcHT reference channel electrode. The invention further discloses a preparation method and an application of the cytochrome C based two-channel ratio type hydrogen peroxide biosensor. By adoptionof the cytochrome C based two-channel ratio type hydrogen peroxide biosensor disclosed by the invention, concentration of hydrogen peroxide can be quantitatively detected.

Description

technical field [0001] The invention belongs to the technical field of biosensor detection, and in particular relates to a dual-channel ratio hydrogen peroxide biosensor based on cytochrome C and its preparation method and application. Background technique [0002] The chemical formula of hydrogen peroxide is H 2 o 2 , pure hydrogen peroxide is a light blue viscous liquid, a strong oxidant, because of its strong oxidizing properties, it is widely used in medical disinfection, industrial production, and daily life. h 2 o 2 It is mainly used to sterilize the surface of objects, and it is also a raw material for chemical production, and it is also an industrial bleaching agent and color developing agent. Not only that, H 2 o 2 As a very important biological reactive oxygen species, it is produced in the process of aerobic synthesis and host defense, and can also be used as a messenger molecule in vertebrates to transmit biological signals (references: Lambert A J, Boysen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 徐红斌叶青
Owner SHANGHAI INST OF QUALITY INSPECTION & TECHN RES
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