Application of spr sensor based on the amplification effect of magnetic molecularly imprinted polymers in the detection of pesticides

A magnetic molecular imprinting and polymer technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of high particle diffusion resistance, difficult elution of template imprinted molecules, and reduce the utilization rate of imprinted sites, so as to improve the binding capacity , good application prospects, fast association/dissociation rate effect

Inactive Publication Date: 2015-11-25
NANCHANG UNIV
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  • Abstract
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Problems solved by technology

At present, the simple and easy bulk polymerization method is the most commonly used method to prepare MIPs. However, the prepared highly cross-linked polymer network, the internal template imprinted molecules are not easy to elute, the internal diffusion resistance of the particle is large, and the imprinted particles embedded in the particle The site cannot function, reducing the utilization of the imprinted site

Method used

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  • Application of spr sensor based on the amplification effect of magnetic molecularly imprinted polymers in the detection of pesticides
  • Application of spr sensor based on the amplification effect of magnetic molecularly imprinted polymers in the detection of pesticides
  • Application of spr sensor based on the amplification effect of magnetic molecularly imprinted polymers in the detection of pesticides

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Experimental program
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Effect test

Embodiment 1

[0026] (1) Fe 3 o 4 Preparation of NPs: 2.35g FeSO 4 ·7H 2 O and 4.1 g FeCl 3 ·6H 2 O was dissolved in 90mL of water, and 25mL of ammonia water with a mass percentage concentration of 25% was quickly added at room temperature. 7nm Fe 3 o 4 NPs. The above experimental process needs to be carried out under the protection of nitrogen. Finally, the Fe in the solution is 3 o 4 NPs were isolated and stored at 4°C for future use;

[0027] (2) Preparation of magnetic molecularly imprinted polymer: 50mg Fe 3 o 4 NPs were dissolved in 20 mL, 10 mM, pH 8.0 Tris buffer solution, 5 mL of acetonitrile solution containing 1 mg / mL CPF was added, mechanically stirred for 2 h at room temperature, then 25 mg DA was added, and stirring was continued for 4 h at room temperature; the reaction product was separated with a magnet, and The mixed solution of 3% acetic acid by volume and 20% acetonitrile by volume eluted the imprinted CPF molecules and unpolymerized DA until the ultraviolet...

Embodiment 2

[0032] Construction of SPR sensing interface

[0033] (1) The construction process of the SPR sensing interface is as follows: figure 1 shown. First place the gold flakes in H with a volume ratio of 7:3 2 SO 4 :H 2 o 2 Soak in the mixed solution for 2 minutes, rinse with secondary water and blow dry with nitrogen, and install it into the SPR detection cell. Inject 50 μL of 1 mM 11-mercaptoundecanoic acid solution into the detection cell to react for 2 hours, rinse with secondary water, inject 50 μL of 0.2M 1-ethyl-3-(3-dimethylaminocarbodiimide) hydrochloride and 0.05MN -Hydroxysuccinimide mixed solution was reacted for 10 min, and then injected with 50 μL 2U / mLAChE solution for 30 min; finally, 50 μL of 1.0M ethanolamine solution was injected for 10 min, and after washing with 10 mM Tris buffer solution of pH 8.5, the AChE functionalized SPR was obtained. sensor interface;

[0034] (2) Combining CPF with MIP-Fe 3 o 4 PDANPs were mixed at room temperature and incubate...

Embodiment 3

[0037] (1) Influence of polymerization time, pH of polymerization solution, and pH of recombination medium solution on sensor performance

[0038] Figure 6 A is the effect of DA aggregation time on sensor performance. It can be seen from the figure that as the polymerization time prolongs, the SPR angle change (ΔAngle) gradually increases. When the polymerization time is 4h, ΔAngle reaches the maximum, and after more than 4h, ΔAngle decreases. If the polymerization time is too short, the number of imprinted sites is small, resulting in weak adsorption capacity for CPF; if the polymerization time is too long, the thickness of the PDA film will be large, and the elution efficiency of CPF will be low, which will lead to a decrease in the adsorption capacity of molecular imprinting. Therefore, the best polymerization time for choosing DA is 4h. Figure 6 B is the effect of the pH of the polymerization solution on the performance of the sensor. Lower pH will inhibit the self-po...

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Abstract

The invention relates to the construction of a surface plasmon resonance sensor based on the amplification effect of magnetic molecularly imprinted polymers and its application in pesticide detection, belonging to the technical field of surface plasmon resonance sensing. It first uses chlorpyrifos as a template molecule, and uses the self-polymerization property of dopamine to synthesize a magnetic molecularly imprinted polymer that can recognize chlorpyrifos. After elution of chlorpyrifos, the magnetic molecularly imprinted polymer containing the imprinted cavity has good superparamagnetism and high selectivity. Using the specific interaction between acetylcholinesterase immobilized on the surface of the gold sheet and chlorpyrifos, a magnetic-based molecularly imprinted polymer was constructed. Molecularly imprinted polymer amplification effect pesticide residue recognition and detection sensing interface; compared with traditional pesticide residue detection technology, the surface plasmon resonance sensor based on the magnetic molecular imprinted polymer amplification effect constructed by the present invention has high detection sensitivity for chlorpyrifos And selectivity is good, has very good application prospect.

Description

technical field [0001] The invention relates to the construction of an SPR sensor based on the amplification effect of magnetic molecularly imprinted polymers and the application of the SPR sensor in pesticide detection, belonging to the technical field of surface plasmon resonance sensing. Background technique [0002] Molecular imprinting technology refers to the preparation technology of preparing polymers that completely match the template molecules in space and binding sites. Molecularly imprinted polymers (MIPs) have been widely used in various scientific fields due to their mechanical / chemical stability, low cost, and ease of preparation. At present, the simple and easy bulk polymerization method is the most commonly used method to prepare MIPs. However, the prepared highly cross-linked polymer network, the internal template imprinted molecules are not easy to elute, the internal diffusion resistance of the particle is large, and the imprinted particles embedded in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/552
Inventor 梁汝萍姚桂红邱建丁
Owner NANCHANG UNIV
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