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Application of self-assembled film in surface auxiliary laser desorption or ionization flying time mass spectrum

A technology of time-of-flight mass spectrometry and self-assembled membranes, which is applied in the direction of material analysis, measuring devices, instruments, etc. through electromagnetic means, can solve the problems of no reports, etc., and achieve the effect of strong response signal and good analysis effect

Inactive Publication Date: 2013-05-22
张琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, in the prior art, neither the use of self-assembled films formed by aromatic thiol compounds on metal surfaces as a film in SALDI-TOF-MS for the analysis of small molecule samples, nor the idea of ​​this technology any inspiration

Method used

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  • Application of self-assembled film in surface auxiliary laser desorption or ionization flying time mass spectrum
  • Application of self-assembled film in surface auxiliary laser desorption or ionization flying time mass spectrum
  • Application of self-assembled film in surface auxiliary laser desorption or ionization flying time mass spectrum

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Embodiment 1

[0035] The present invention provides the application of self-assembled film in surface-assisted laser desorption / ionization time-of-flight mass spectrometry, which is characterized in that the self-assembled film of aromatic thiol compound is used for surface-assisted laser desorption / ionization time-of-flight mass spectrometry (SALDI) for small molecules. -TOF MS).

[0036] Contains the following process:

[0037] (1) Preparation of self-assembled films of aromatic thiol compounds for surface-assisted laser desorption / ionization of small molecules. The preparation includes the following steps:

[0038] (A) First, ultrasonically clean the silicon wafer in a toluene solution to remove surface contamination, then use a diluted hydrofluoric acid solution to corrode the contamination on the silicon surface, and finally rinse the silicon wafer repeatedly with deionized water to obtain a Processed silicon wafers;

[0039] (B) First, use a magnetron radio frequency sputtering device to spu...

Embodiment 2

[0046] The present invention provides the application of self-assembled film in surface-assisted laser desorption / ionization time-of-flight mass spectrometry, which is characterized in that the self-assembled film of aromatic thiol compound is used for surface-assisted laser desorption / ionization time-of-flight mass spectrometry (SALDI) for small molecules. -TOF MS).

[0047] Contains the following process:

[0048] (1) Preparation of self-assembled films of aromatic thiol compounds for surface-assisted laser desorption / ionization of small molecules. The preparation includes the following steps:

[0049] (A) First, ultrasonically clean the silicon wafer in a toluene solution to remove surface contamination, then use a diluted hydrofluoric acid solution to corrode the contamination on the silicon surface, and finally rinse the silicon wafer repeatedly with deionized water to obtain a Processed silicon wafers;

[0050] (B) First, use a magnetron radio frequency sputtering device to spu...

Embodiment 3

[0055] The present invention provides the application of self-assembled film in surface-assisted laser desorption / ionization time-of-flight mass spectrometry, which is characterized in that the self-assembled film of aromatic thiol compound is used for surface-assisted laser desorption / ionization time-of-flight mass spectrometry (SALDI) for small molecules. -TOF MS).

[0056] Contains the following process:

[0057] (1) Preparation of self-assembled films of aromatic thiol compounds for surface-assisted laser desorption / ionization of small molecules. The preparation includes the following steps:

[0058] (A) Pretreatment of silicon wafers;

[0059] (B) Sputtering Ti and Au sequentially on the pre-treated silicon wafer to obtain a gold substrate; and

[0060] (C) Put the gold substrate in the alcohol solution of the aromatic thiol compound for self-assembly reaction, take it out after sufficient reaction time, rinse with alcohol and dry, to obtain the self-assembled film of the aromatic...

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Abstract

The invention discloses application of a self-assembled film in a surface auxiliary laser adsorption or ionization flying time mass spectrum. The application is characterized in that the invention relates to the application of the self-assembled film made of an aromatic thiol compound in the surface auxiliary laser adsorption or ionization flying time mass spectrum. The application comprises the following steps of: firstly, preparing the aromatic thiol compound self-assembled film for surface auxiliary laser desorption or ionization of small molecules, namely preprocessing a silicon slice, sequentially sputtering Ti and Au on the preprocessed silicon slice to obtain a golden substrate, and putting the golden substrate into an ethanol solution with the aromatic thiol compound for generating self-assembling reaction to obtain the aromatic thiol compound self-assembled film; and then drying small-molecular sample points on the surface of the obtained self-assembled film, and placing the self-assembled film into an MALDI-TOF-MS mass spectrum for analysis. The application has the advantages that the substrate is stable; an Au layer is difficult to fall off; the small peptide and the traditional Chinese medicine active components can be conveniently and quickly analyzed; an analysis effect is good; and a response signal is high.

Description

Technical field [0001] The invention relates to a surface-assisted laser desorption / ionization time-of-flight mass spectrometer, in particular to the application of a self-assembled film in a surface-assisted laser desorption / ionization time-of-flight mass spectrometer. Background technique [0002] Matrix-assisted laser desorption / ionization-time of flight mass spectrometry (MALDI-TOF-MS) has sensitive, convenient, good soft ionization characteristics, anti-interference ability against impurities, and can directly The feature of analyzing mixtures without pre-separation, the use of MALDI-TOF-MS technology to analyze low molecular weight compounds has attracted more and more attention. At present, most commonly used commercial substrates are organic compounds with small molecules (under 500 Da), such as 2,5-dihydroxybenzoic acid (DHB) or α-cyano-4-hydroxycinnamic acid (CHCA) and its derivatives. However, in the MALDI analysis of small molecule compounds, commonly used small mole...

Claims

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Application Information

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IPC IPC(8): G01N27/64
Inventor 张琦
Owner 张琦
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