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Preparation method and applications of current mode immuno-sensor for detecting breast cancer

An immunosensor, breast cancer technology, used in instruments, measuring devices, scientific instruments, etc., can solve problems such as weak conductivity, and achieve the effects of rapid detection, high conductivity, and improved sensitivity

Inactive Publication Date: 2018-06-29
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Copper oxide to H 2 o 2 The reduction of copper oxide shows good catalytic performance, but because copper oxide is a semiconductor, its conductivity is weak

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 prepares copper oxide functionalized graphene nanocomposite material

[0042] ① Preparation of graphene oxide

[0043] Add 0.4 g graphite powder into 80.0 mL, 98.0 wt% concentrated sulfuric acid and 6.0 mL concentrated phosphoric acid mixed solution, continue to stir for 25.0 min, continue to add 3.0 g potassium permanganate, continue to stir evenly, heat to 50.0 °C, and react After 12.0 h, add 30.0 mL of ultrapure water into ice, continue to add 0.4 mL of 20.0 wt% hydrogen peroxide, continue stirring for 20.0 min, centrifuge, wash with 25.0 mL of ultrapure water, and centrifuge to separate the obtained supernatant Freeze-drying obtains graphene oxide;

[0044] ②Preparation of copper oxide hybrid graphene nanocomposites

[0045] Add 0.1 g of graphene oxide to 100.0 ml of water, continue to add 0.5 g of copper nitrate trihydrate, and then after ultrasonication for 30.0 min, add 10.0 ml of ammonia water, stir for 20.0 min, and transfer the solution to a hig...

Embodiment 2

[0048] Example 2 Preparation of copper oxide functionalized graphene nanocomposites

[0049] ① Preparation of graphene oxide

[0050] Add 0.5 g graphite powder into 70.0 mL, 98.0 wt% concentrated sulfuric acid and 7.0 mL concentrated phosphoric acid mixed solution, continue stirring for 25.0 min, continue to add 4.0 g potassium permanganate, continue stirring evenly, heat to 60.0 °C, and react After 12.0 h, add 35.0 mL of ultrapure water into ice, continue to add 0.5 mL of 25.0 wt% hydrogen peroxide, continue to stir for 20.0 min, centrifuge, wash with 30.0 mL of ultrapure water, and centrifuge to separate the obtained supernatant Freeze-drying obtains graphene oxide;

[0051] ②Preparation of copper oxide hybrid graphene nanocomposites

[0052] Add 0.2 g of graphene oxide into 100.0 ml of water, continue to add 1.0 g of copper nitrate trihydrate, and then after ultrasonication for 30.0 min, add 10.0 ml of ammonia water, stir for 20.0 min, and transfer the solution to a high-...

Embodiment 3

[0055] Example 3 Preparation of copper oxide functionalized graphene nanocomposites

[0056] ① Preparation of graphene oxide

[0057] Add 0.6 g graphite powder into 90.0 mL, 98.0 wt% concentrated sulfuric acid and 8.0 mL concentrated phosphoric acid mixed solution, continue stirring for 25.0 min, continue to add 5.0 g potassium permanganate, continue stirring evenly, heat to 70.0 °C, and react After 12.0 h, add 40.0 mL of ultrapure water into ice, continue to add 0.6 mL of 30.0 wt% hydrogen peroxide, continue to stir for 20.0 min, centrifuge, wash with 35.0 mL of ultrapure water, and centrifuge to separate the obtained supernatant Freeze-drying obtains graphene oxide;

[0058] ②Preparation of copper oxide hybrid graphene nanocomposites

[0059] Add 0.3 g of graphene oxide into 100.0 ml of water, continue to add 1.5 g of copper nitrate trihydrate, and then after ultrasonication for 30.0 min, add 10.0 ml of ammonia water, stir for 20.0 min, and transfer the solution to a high-...

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PUM

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Abstract

The invention belongs to the fields of novel nano materials, immuno-assay, and biosensor, provides a preparation method and applications of a current mode immuno-sensor for detecting breast cancer, and specifically relates to a construction method of an electrochemical immuno-sensor, which is made of a graphene nano composite material based on palladium loaded nano rod copper oxide functionalization, and an application of using the electrochemical immuno-sensor to detect a specific antigen of breast cancer.

Description

technical field [0001] The invention belongs to the technical fields of novel nanomaterials, immune analysis and biosensing, and provides a preparation method and application of an amperometric immunosensor for detecting breast cancer, and specifically relates to a graphene based on palladium-loaded nanorod copper oxide functionalization A method for constructing an electrochemical immunosensor of a nanocomposite material, and an application of the electrochemical immunosensor constructed by the method in detecting breast cancer-specific antigens. Background technique [0002] Breast cancer is the most common malignancy in women and the second leading cause of cancer death. Breast cancer is one of the most serious health problems for women around the world. Therefore, early diagnosis through specific and sensitive biomarkers and analysis tools can reduce the incidence of breast cancer and effectively improve the survival rate. Therefore, it is of great significance to be ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/308G01N27/3278
Inventor 王平马恩慧李明党裴福彬余昊轩董云会李月云
Owner SHANDONG UNIV OF TECH
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