Preparation method and applications of current mode immuno-sensor for detecting breast cancer
An immunosensor, breast cancer technology, used in instruments, measuring devices, scientific instruments, etc., can solve problems such as weak conductivity, and achieve the effects of rapid detection, high conductivity, and improved sensitivity
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Embodiment 1
[0041] Embodiment 1 prepares copper oxide functionalized graphene nanocomposite material
[0042] ① Preparation of graphene oxide
[0043] Add 0.4 g graphite powder into 80.0 mL, 98.0 wt% concentrated sulfuric acid and 6.0 mL concentrated phosphoric acid mixed solution, continue to stir for 25.0 min, continue to add 3.0 g potassium permanganate, continue to stir evenly, heat to 50.0 °C, and react After 12.0 h, add 30.0 mL of ultrapure water into ice, continue to add 0.4 mL of 20.0 wt% hydrogen peroxide, continue stirring for 20.0 min, centrifuge, wash with 25.0 mL of ultrapure water, and centrifuge to separate the obtained supernatant Freeze-drying obtains graphene oxide;
[0044] ②Preparation of copper oxide hybrid graphene nanocomposites
[0045] Add 0.1 g of graphene oxide to 100.0 ml of water, continue to add 0.5 g of copper nitrate trihydrate, and then after ultrasonication for 30.0 min, add 10.0 ml of ammonia water, stir for 20.0 min, and transfer the solution to a hig...
Embodiment 2
[0048] Example 2 Preparation of copper oxide functionalized graphene nanocomposites
[0049] ① Preparation of graphene oxide
[0050] Add 0.5 g graphite powder into 70.0 mL, 98.0 wt% concentrated sulfuric acid and 7.0 mL concentrated phosphoric acid mixed solution, continue stirring for 25.0 min, continue to add 4.0 g potassium permanganate, continue stirring evenly, heat to 60.0 °C, and react After 12.0 h, add 35.0 mL of ultrapure water into ice, continue to add 0.5 mL of 25.0 wt% hydrogen peroxide, continue to stir for 20.0 min, centrifuge, wash with 30.0 mL of ultrapure water, and centrifuge to separate the obtained supernatant Freeze-drying obtains graphene oxide;
[0051] ②Preparation of copper oxide hybrid graphene nanocomposites
[0052] Add 0.2 g of graphene oxide into 100.0 ml of water, continue to add 1.0 g of copper nitrate trihydrate, and then after ultrasonication for 30.0 min, add 10.0 ml of ammonia water, stir for 20.0 min, and transfer the solution to a high-...
Embodiment 3
[0055] Example 3 Preparation of copper oxide functionalized graphene nanocomposites
[0056] ① Preparation of graphene oxide
[0057] Add 0.6 g graphite powder into 90.0 mL, 98.0 wt% concentrated sulfuric acid and 8.0 mL concentrated phosphoric acid mixed solution, continue stirring for 25.0 min, continue to add 5.0 g potassium permanganate, continue stirring evenly, heat to 70.0 °C, and react After 12.0 h, add 40.0 mL of ultrapure water into ice, continue to add 0.6 mL of 30.0 wt% hydrogen peroxide, continue to stir for 20.0 min, centrifuge, wash with 35.0 mL of ultrapure water, and centrifuge to separate the obtained supernatant Freeze-drying obtains graphene oxide;
[0058] ②Preparation of copper oxide hybrid graphene nanocomposites
[0059] Add 0.3 g of graphene oxide into 100.0 ml of water, continue to add 1.5 g of copper nitrate trihydrate, and then after ultrasonication for 30.0 min, add 10.0 ml of ammonia water, stir for 20.0 min, and transfer the solution to a high-...
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