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A urine iodine quantitative detection kit

A technology for quantitative detection and detection of reagents, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc. problems, to achieve the effect of shortening the detection time and improving the detection efficiency

Active Publication Date: 2020-08-25
天津中成佳益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the technologies used at home and abroad for the determination of urinary iodine include arsenic-cerium catalytic spectrophotometry, plasma chromatography mass spectrometry and chemiluminescence. These methods all need to digest the samples in advance, and the digestion time is long and time-consuming.

Method used

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  • A urine iodine quantitative detection kit
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  • A urine iodine quantitative detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The concrete composition of the liquid double reagent of a kind of urine iodine quantitative detection kit is as follows:

[0048] Urine iodine reagent R1

[0049]

[0050] Urine iodine reagent R2

[0051]

[0052] Urine iodine calibrator

[0053] Sodium Azide 0.5%,

[0054] Pure potassium iodate.

[0055] The reagents in this example adopt Roche C311 automatic biochemical analyzer, and the comparison method is the WST107-1999 urine iodine-large arsenic-cerium catalytic spectrophotometric method. At the same time, 20 samples are measured according to their respective parameters, and the measured values ​​are correlated. , see the result figure 1 , what the X-axis represents is the measured value of the reagent of the present invention, and what the Y-axis represents is the measured value of the national standard method. Correlation coefficient: r2=0.9984, the linear equation is y=1.0323x-5.629, the results show that the reagents of this example have a good co...

Embodiment 2

[0057] Urine iodine reagent R1

[0058]

[0059]

[0060] Urine iodine reagent R2

[0061]

[0062] Urine iodine calibrator

[0063] Sodium Azide 0.5%,

[0064] Pure potassium iodate.

[0065] Select a high-value sample with a UI value of about 1000ug / l, dilute it into different concentrations, and use the reagent described in this example to verify the linearity. The linear range is 5-1000ug / l. For the linear range verification results, see figure 2 , the correlation coefficient is 0.9993, and the linear range of the reagent in this embodiment can reach 5-1000ug / l.

Embodiment 3

[0067] Urine iodine reagent R1

[0068]

[0069]

[0070] Urine iodine reagent R2

[0071]

[0072] Urine iodine calibrator

[0073] Sodium Azide 0.5%,

[0074] Pure potassium iodate.

[0075]Carry out precision verification with the reagent of this example, continuously measure 20 times to the same sample, calculate its CV value, according to the result of Table 1, the precision of the reagent of the present invention is controlled within the scope of 5%, has good precision.

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Abstract

The invention provides a kit for quantitatively detecting urine iodine. The kit for quantitatively detecting urine iodine is characterized in that a detecting reagent in the kit is prepared from a urine iodine reagent R1, a urine iodine reagent R2 and a urine iodine calibration material, wherein the urine iodine reagent R1 is prepared from the following components: 0.1 to 1.50 mol / L of concentrated sulfuric acid, 0.5 to 2.0 mol / L of ammonium persulfate, 10 to 20 mmol / L of sodium chloride, 20 to 50 mmol / L of arsenic trioxide, 0.01 to 0.05 mol / L of catalyst I and 0.05 percent of preservative. The kit for quantitatively detecting urine iodine, provided by the invention, is suitable for detection and use of a full-automatic biochemical analyzer, a sample requires not to be subjected to digestive treatment in advance, the full-automatic biochemical analyzer can be directly used for determining, the determination time of the sample just requires 10 minutes, the detection time is greatly shortened, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a urine iodine quantitative detection kit. Background technique [0002] Iodine is an essential trace element for the rapid synthesis of thyroid gland. Insufficient or excessive iodine intake by the body will affect thyroid function, cause thyroid damage, and lead to thyroid diseases. The kidney is the main excretion organ of iodine, and 90% of iodine is excreted through urine and 10% is excreted through feces. Urinary iodine level is an important index to directly evaluate the iodine nutritional status of human body. The quantitative determination of urinary iodine provides an important scientific basis for diagnosing whether people are iodine deficient or iodine intake is excessive. [0003] Iodine deficiency during pregnancy will reduce the thyroid synthesis of pregnant women and fetuses, and the physical and intellectual development of infants and young children will be directl...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 郑丽丽
Owner 天津中成佳益生物科技有限公司
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