Urine iodine detection method

A detection method, urinary iodine technology, applied in the field of iodine ion detection, can solve the problem of low accuracy of detection results

Inactive Publication Date: 2020-12-15
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The main purpose of the present invention is to propose a urine iodine detection method, aiming to solve the problem that the accuracy of the detection result is not high due to the naked eye judgment of the reaction end point in the existing arsenic and cerium catalytic timing measurement method

Method used

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Experimental program
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Effect test

Embodiment 1

[0057] (1) Use a pipette to take 0.2ml of urine sample into a transparent circular test tube with a diameter of 1cm, add 1ml of sodium hypochlorite solution with a chlorine content of 1mol / L to the urine sample, and shake the test tube vigorously after standing for 5 minutes. Minutes to obtain the digested urine sample;

[0058] (2) Add 1 mL of urea solution with a concentration of 0.8 mol / L to the digested urine sample, let it stand for 2 minutes, then shake the test tube vigorously for 5 minutes to obtain the analyzed urine sample;

[0059] (3) Add 1 mL of sulfuric acid solution with a hydrogen ion concentration of 5 moL / L to the analyzed urine sample, shake the test tube vigorously for 10 minutes until no gas is generated, and obtain the pretreated urine sample;

[0060] (4) Add 0.5ml of arsenous acid solution with arsenic content of 0.05moL / L to the pretreated urine sample and shake well, then add 0.5ml of o-phenanthroline solution with arsenic content of 0.04mol / L mL, an...

Embodiment 2

[0066] (1) Use a pipette to take 0.2ml of urine sample into a transparent circular test tube with a diameter of 1cm, add 1ml of sodium hypochlorite solution with a chlorine content of 1.2mol / L to the urine sample, and shake the test tube vigorously after standing for 5 minutes 7 minutes to obtain the urine sample after digestion;

[0067] (2) Add 1.5 mL of urea solution with a concentration of 1 mol / L to the digested urine sample, let it stand for 2 minutes and shake the test tube vigorously for 7 minutes to obtain the analyzed urine sample;

[0068] (3) Add 1 mL of sulfuric acid solution with a hydrogen ion concentration of 6 moL / L to the analyzed urine sample, shake the test tube vigorously for 10 minutes until no gas is generated, and obtain the pretreated urine sample;

[0069] (4) Add 0.6ml of arsenous acid solution with an arsenic content of 0.02moL / L to the pretreated urine sample and shake well, then add 0.5ml of an o-phenanthroline solution with a content of 0.04mol / L...

Embodiment 3

[0075] (1) Use a pipette to take 0.2ml of urine sample into a transparent circular test tube with a diameter of 1cm, add 1ml of sodium hypochlorite solution with a chlorine content of 2mol / L to the urine sample, let it stand for 5 minutes, and shake the test tube vigorously for 10 minutes Minutes to obtain the digested urine sample;

[0076] (2) Add 2 mL of urea solution with a concentration of 1 mol / L to the digested urine sample, let it stand for 2 minutes and shake the test tube vigorously for 7 minutes to obtain the analyzed urine sample;

[0077] (3) Add 2 mL of sulfuric acid solution with a hydrogen ion concentration of 6 moL / L to the analyzed urine sample, shake the test tube vigorously for 10 minutes until no gas is generated, and obtain the pretreated urine sample;

[0078] (4) Add 0.3ml of arsenous acid solution with an arsenic content of 0.08moL / L to the pretreated urine sample and shake well, then add 0.5ml of an o-phenanthroline solution with a content of 0.04mol / L....

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Abstract

The invention discloses a urine iodine detection method which comprises the following steps of: pretreating a urine sample to prepare a pretreated urine sample; adding a reducing agent, a phenanthroline solution and an oxidizing agent into the pretreated urine sample to prepare a mixed solution, wherein the reducing agent is an arsenious acid solution, and the oxidizing agent is an ammonium ceroussulfate solution or a cerous sulfate solution; taking a laser detector to emit laser with a preset wavelength to the mixed solution, collecting transmission light intensity in real time, and startingtiming; when the transmitted light intensity reaches a preset threshold value, stopping timing, and taking a time period from timing starting to timing stopping as reaction time; and calculating theiodine content in the urine sample according to the reaction time and a preset iodine content and time standard relation curve regression equation. The urine iodine detection method solves the problemof low detection result accuracy degree caused by naked eye judgment of a reaction endpoint in an existing arsenic-cerium catalytic timing determination method.

Description

technical field [0001] The invention relates to the technical field of iodine ion detection, in particular to a urine iodine detection method. Background technique [0002] In the field of health and medicine, the detection of iodine ions is mainly the detection of urine iodine and water iodine. Urinary iodine is an important indicator for the monitoring of iodine deficiency disease prevention and control and the evaluation of iodine nutrition level in the population, which has been widely used at home and abroad. The urinary iodine level of the population can reflect whether the iodine intake of the population is iodine deficiency, iodine appropriate or iodine excess. The monitoring of the population's urinary iodine level is an important basis for guiding the population to supplement iodine scientifically, and has important public health significance. For pregnant women, iodine deficiency can lead to decreased synthesis of thyroid hormones in the mother and fetus, affect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/59G01N1/44
CPCG01N21/78G01N21/59G01N1/44
Inventor 赵荣丽王中任刘海生徐可
Owner HUBEI UNIV OF ARTS & SCI
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