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Method for detecting ponceaux 2R in food

A detection method, food technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of easy blockage of capillary, interference of analysis and measurement, large residual current, etc., and achieve simplified sample processing procedures, reduced solvent consumption, and high selectivity Effect

Inactive Publication Date: 2008-05-07
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The generation of polarographic waves is caused by the concentration polarization on the polarized electrode (i.e. the mercury drop electrode). The main disadvantage of this method is that the measurement results are easily interfered by high-concentration cations, and the ions in many foods The concentration is high; the mercury vapor generated by the mercury dropping electrode is poisonous to humans; when the mercury dropping electrode is used as an anode, the potential must not be greater than 0.4V (Vs SCE), otherwise the mercury will be oxidized; the residual current is large and some abnormal currents often appear. Interfering; used capillaries are prone to clogging

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Detection of Ponceau 2R in commercially available chili powder

[0018] 1. Determine the testing standard

[0019] A. Qualitative testing standards:

[0020] Take the Ponceau 2R standard product dissolved in ultrapure water, and then dissolve it in a volumetric flask to prepare a standard stock solution with a concentration of 0.5-2.0 mg / mL. Dilute the Ponceau 2R standard stock solution with ultrapure water to prepare The standard solution with a concentration range of 0.1-15μg / mL, after filtration with a 0.45μm filter membrane, the chromatographic column is XDB-C18 (150mm×4.6mmi.d.5μm); mobile phase A: 0.005mol / L ammonium acetate NH 4 Ac+0.06% triethylamine aqueous solution, adjust the pH to 8 with 0.1mol / L glacial acetic acid, B: chromatographically pure acetonitrile CH 3CN; Elution gradient: t=0 minutes, 10% B; t=2 minutes, 10% B; t=15 minutes, 90% B; t=17 minutes, 10% B; flow rate 1.0mL / min, detection wavelength Under the chromatographic analysis condi...

Embodiment 2

[0029] Example 2: Detection of Ponceau 2R in commercially available hard candies

[0030] 1, determine the standard of detection: its qualitative detection, quantitative detection standard are with embodiment 1

[0031] 2. Sample processing

[0032] Accurately weigh 2g of the sample, put it into a 200mL beaker, dissolve it in hot ultrapure water at 70°C, dilute it with ultrapure water, and put it in a 25mL volumetric flask. Take 6mL of the diluted solution and centrifuge at 6000r / min for 20min, and absorb the supernatant 3mL spare.

[0033] The weak anion solid-phase extraction column is first activated with the chromatographically pure methanol, and then activated with 3mL ultrapure water. Wash the solid-phase extraction column with water and 3mL of methanol solvent, enter the air-dried extraction column for 30 seconds, and finally use 3mL of chromatographically pure methanol containing 2% pure ammonia water for elution, and collect the eluate. The eluting solution was blo...

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Abstract

The invention discloses a detection method of Ponceau 2R in food. The non-water-soluble sample to be tested is extracted with an ethanol-ammonia solution in an ultrasonic generator, and the water-soluble sample is dissolved in hot water to extract the supernatant. Then extract with a weak anion solid-phase extraction column, analyze the extract with high-performance liquid chromatography, and determine whether it contains Ponceau 2R according to the retention time of the chromatographic peak; further use the standard addition recovery method to calculate the actual sample according to the linear equation concentration, and then determine the content of Ponceau 2R according to the detection amount, the detection limit is 0.02μg / ml, the relative standard deviation is 0.33-2.86%, and the recovery rate of standard addition is 90.05-94.91%. The present invention can significantly Reduce the amount of solvent and simplify the sample processing procedures, effectively remove endogenous interfering substances, which are not subject to the subjective influence of the tester, the steps are simple, the sensitivity is high, and the detection is accurate.

Description

1. Technical field [0001] The invention belongs to a detection method of Ponceau. 2. Background technology [0002] Ponceau 2R is an azo dye synthesized from raw materials extracted from coal tar, also known as "acid scarlet", and its molecular formula is C 18 h 14 N 2 Na 2 o 7 S 2 , was once used as food dye, medicine and cosmetic dye, and later found that Ponceau 2R has a toxic effect on the liver of humans and animals, and can cause liver degenerative diseases, liver cirrhosis and other toxic symptoms. It has been completely banned internationally for food dyeing. But Ponceau 2R has good dyeing effect and strong fastness, which makes the food full of freshness and can arouse people's appetite and desire to buy. It has been reported that some food producers illegally added Ponceau 2R to improve food color. At present, there is no national standard recommended detection method for Ponceau 2R in food. The reported detection methods for Ponceau 2R are: (1) Spot method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88
Inventor 赵志磊吴广臣李小亭庞艳苹
Owner HEBEI UNIVERSITY
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