Kit for determining urine iodine and preparation and use method thereof
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A kit and reagent technology, applied in the fields of medicine and biochemistry, can solve the problems of slow testing, the operator's proficiency has a great influence on the results, and the inability to carry out and popularize on a large scale, so as to simplify the experimental steps and shorten the reaction time. Effect
Inactive Publication Date: 2018-02-09
ANHUI IPROCOM BIOTECH CO LTD
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Problems solved by technology
However, this method has many disadvantages: the reaction time is about 90 minutes, it cannot be applied to automatic biochemical analyzers, manual operation is required, the test is slow, and the proficiency of the operator has a great influence on the results, so it cannot be carried out and promoted on a large scale
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Embodiment 1
[0063] A kit for determining urinary iodine in this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:
[0064] Reagent R1:
[0065]
[0066] The solvent is purified water;
[0067] Reagent R2:
[0068] Tris buffer 20mmol / L
[0069] Ammonium cerium sulfate 20g / L
[0070] h 2 SO 4 2.0mol / L
[0071] Its solvent is purified water.
Embodiment 2
[0073] A kit for determining urinary iodine in this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:
[0074] Reagent R1:
[0075]
[0076] The solvent is purified water;
[0077] Reagent R2:
[0078] Tris buffer 180mmol / L
[0079] Ammonium cerium sulfate 40g / L
[0080] h 2 SO 4 3.0mol / L
[0081] Its solvent is purified water.
Embodiment 3
[0083] A kit for determining urinary iodine in this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:
[0084] Reagent R1:
[0085]
[0086] The solvent is purified water;
[0087] Reagent R2:
[0088] Tris buffer 100mmol / L
[0089] Ammonium cerium sulfate 30g / L
[0090] h 2 SO 4 2.5mol / L
[0091] Its solvent is purified water.
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Abstract
The invention discloses a kit for determining urine iodine. The kit comprises double-liquid components reagents R1 and R2 which are independent mutually, wherein the reagent R1 is prepared from 20-180mmol/L of Tris buffer solution, 10-30 g/L of NaCl, 2-30 g/L of Sb2O3, 2.0-3.0 mol/L of H2SO4 and a solvent purified water, and the reagent R2 is prepared from 20-180 mmol/L of Tris buffer solution, 20-40 g/L of ammonium cerous sulfate, 2.0-3.0 mol/L of H2SO4 and a solvent purified water. The invention also discloses a preparation and use method of the kit for determining urine iodine. The kit hasthe advantages that urine does not need digestive treatment, and experimental steps are simplified; only 10 minutes are needed for detection, the reaction time is greatly compressed; the kit is suitable for full-automatic testing.
Description
technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring urinary iodine suitable for automatic biochemical analysis and a preparation method thereof. Background technique [0002] The critical period of human brain development, one is 10-18 weeks of gestational age, which is the period when neuroblasts proliferate, develop, and form brain tissue; the other is 3 months before birth to 2 years after birth, which is the main stage of brain development and maturity During this period, iodine and thyroid hormones play a decisive role in the development and proliferation of brain cells, and it is the most effective period of iodine supplementation in life. For the vast majority of people, iodine deficiency will result in suboptimal intellectual development, and IQ will be on the edge, middle, or middle level of the normal range. [0003] Large-scale surveys around the world have shown that children bor...
Claims
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