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Iodide ion detection kit

A detection kit and iodine ion technology, which is applied in the field of iodine ion detection, can solve the problems of excessive discharge of harmful substances, long analysis time, complex and cumbersome operation, etc., and achieve the effect of preventing steam leakage and ensuring accuracy

Inactive Publication Date: 2019-12-13
张鹏
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  • Application Information

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Problems solved by technology

At present, "ammonium persulfate digestion-arsenic-cerium catalytic spectrophotometry" for urine iodine determination is widely used in countries all over the world. It is very simple and easy to detect large quantities of urine iodine, but it is complicated and cumbersome to detect urine iodine in clinical samples. Large, long analysis time, more harmful substances discharge

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Embodiment

[0034] Example: Detect iodine content in urine

[0035] The iodide ion detection method and reagent of the present invention are used for determination.

[0036] (1) Reagents

[0037] (1.1) Reagent R1: ammonium persulfate solution, the concentration is 1.0mol / L;

[0038] (1.2) Reagent R2: Ferroin solution, wherein the concentration of ferrous ammonium sulfate is 0.025mol / L, and the concentration of o-phenanthroline is 0.020mol / L;

[0039] (1.3) Reagent R3: arsenous acid solution, concentration 0.040mol / L;

[0040] (1.4) Reagent R4: the concentration of ammonium cerium sulfate solution is 0.008 mol / L;

[0041] (1.5) Iodine standard series solutions: Potassium iodate solution, iodine concentrations are: 50, 100, 200, 300, 400, 500 μg / L.

[0042] (2) Solution to be tested: Take 25 μL each of the iodine standard series solution and urine sample and place them in 1 mL conical centrifuge tubes, add 125 μL of reagent R1, vortex and mix, place in a dry thermostat at 100°C and dige...

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Abstract

The invention discloses an iodide ion detection kit based on iodine catalyzed arsenic-cerium reaction under the acidic condition and taking a Ferrion solution as a redox indicator. The kit comprises adigestion solution, a reducing agent, an oxidizing agent and an indicator; an ammonium persulfate or acidic potassium permanganate solution serves as the digestion solution; the arsenious acid solution serves as the reducing agent; a tetravalent cerium solution serves as the oxidizing agent; and a Ferroin reagent solution serves as the indicator. An iodine standard series solution is prepared, and the ammonium persulfate or acidic potassium permanganate solution is used as the digestion solution, so that the defects of dense smoke and explosiveness of chloric acid digestion are overcome. Thedetection kit also has the advantages of being simple, rapid, specified, sensitive and accurate, can be widely applied to the fields in which the content of iodide needs to be detected, and in particular, to urine iodine and water iodine detection in the fields of hygiene inspection and medical inspection.

Description

technical field [0001] The invention belongs to the technical field of iodine ion detection, relates to reagents and detection methods for detecting urine iodine and water iodine in the field of sanitary inspection and medical inspection, and specifically relates to a method based on iodine catalyzing the reaction of arsenic and cerium under acidic conditions and using Ferrion solution for oxidation Iodide Ion Assay Kit for Reducing Indicators. Background technique [0002] In the field of health and medicine, the detection of iodine ions is mainly the detection of urine iodine and water iodine. Urinary iodine is an important indicator for the monitoring of iodine deficiency disease prevention and control and the evaluation of iodine nutrition level in the population, which has been widely used at home and abroad. The urinary iodine level of the population can reflect whether the iodine intake of the population is iodine deficiency, iodine appropriate or iodine excess. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 张鹏
Owner 张鹏
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